| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,416,622 | C→G | Q626E (CAG→GAG) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,416,622 | 0 | C | G | 97.1% | 84.4 / NA | 35 | Q626E (CAG→GAG) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (16/18); minor base T (0/1); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCG > NC_000913/2416491‑2416752 | tCGAACCACGCGTTGCTTTTCTTGCCTACTCCTCCTTTACTTCTTTTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCt < 1:363344/143‑1 (MQ=255) gAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGc > 1:703571/1‑143 (MQ=255) tGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGACAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GCAATGGCTg > 1:827960/1‑143 (MQ=255) gCTATGTTCTCCTACTCCACGAGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGa < 2:140433/143‑1 (MQ=255) gCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTAGCGAAGCAACTCATCTGGCGCAGGAAACACGTCCTGACCTGATGATAGACCGTCCGAGGGAGTACGACGCTGCG‑GCAGTGGCCGa > 2:741324/1‑143 (MQ=255) tGCTCTCCTTCTCCTCCGTTATTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGtt < 1:172028/143‑1 (MQ=255) gCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTg > 2:168053/1‑143 (MQ=255) tcCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGCCGGTTTGTTGGACCCCGACGCATCG‑GTAATGGCTGACGTTGCGaa > 1:782734/1‑143 (MQ=255) tcCTACTCCACCGGTACTTCTGGTGCAGGCAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCACGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTCATGGCTGACGTCGCGaa > 2:493409/1‑143 (MQ=255) tACTCCACCTGTAATTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAATCAACTCTTCTGGCTCAGGAAAAACGTCCTGACCTGATCATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATc < 1:239830/143‑1 (MQ=255) ccACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGCAGCAACTCGTCTGGCGCCGGAACACCGTCCTGCCCTCATGATCGACGGTCCGCTGGAGTACGAAAAAAAG‑GAAATAGCAGACGTTACGAAATACaaa > 1:5452/1‑143 (MQ=255) cACCTGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTGCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAg < 2:501081/143‑1 (MQ=255) aCCGGTACTTCTGGTGCTGTTAGCGACGTATATATATTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAgc < 1:150524/143‑1 (MQ=255) tACTTCTGGTGCAGGTTTCTACGTATAATATTTTCTTGAATCAACTCGTCTGGCGCAGGAAAGACGTCCGGACCTGAGGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGa < 1:166705/143‑1 (MQ=255) gTGCAGGTAGCGACGGAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCg < 2:120625/143‑1 (MQ=255) aGGTAGCGACGTAGAAAAAGTTCGCTAAGCAACCCGACTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGCCGTTGCGCAATCCAAAACGCCGAACGCCCCGGtac > 2:494216/1‑141 (MQ=255) gTAGCGTCGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCa < 1:508996/143‑1 (MQ=255) gTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAGAACGTCCTGACCTGATGATCGACGGTCCGATGGAGTAAGACGATGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCa > 1:189201/1‑143 (MQ=255) aCGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAGATCCAAAGCGCCGAACTCTCCGGTTGCAGGTcgc > 1:140079/1‑143 (MQ=255) cGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGACAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTATGGTGGTGAGCACAACCCCTG‑TGTTGCGAAATCCCAAGCCCCGAACTCCCCGCTTGCAGGCcgca > 1:368178/1‑142 (MQ=255) aaaaGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCg > 2:365061/1‑143 (MQ=255) cgAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCACAGCGCCGAACTCTCCGGCTGCAGGTCCTGCTACCGTGTTCGTCt > 2:300313/1‑143 (MQ=255) aaGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCAGCTGGAGTACGACCCAACG‑GTAATGGCTGACGTTGCGAAATCCAAACCGCCGAACTCTCCGGTTGCAACTCGCACTACCGAGTTCatcatc > 2:402523/1‑143 (MQ=255) aGCACCTCGTCTTGCGCAGGAAAATCGTCCTGACCTGATGTTCGATGGTCCGCTGGAGTACTACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTcc < 1:218237/143‑1 (MQ=255) aGCAACTCTTCTGGTGCAGGAAAAACGTCATGACCTGATGATCGACGGTCTGCTGGAGTACGACGCTGCG‑GGAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTcc < 1:537466/143‑1 (MQ=255) aCTCGTCTGGCGCTGTAAAAACGTCCTGACCTGATGATCGACGGTCTGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGa < 1:772543/143‑1 (MQ=255) cTCGTCTGGTGAAGGAAAAACGTCCTGACCTGATGATCGATGTTCCGCTGTAGTACTACGCTGCG‑GTAATTGCTGACCTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAt < 1:441845/143‑1 (MQ=255) tCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGTTGGAGTATGACGTTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATc < 1:748205/143‑1 (MQ=255) cGTCTGGCGCATGTATAATGTCTTGTTCTGATGAGCGACGGTCAGGTGGAGTACGACGCGGCG‑GTAGTGGCTGACGTTGCGAAATCCAAAGCGGCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCt < 1:304861/143‑1 (MQ=255) gccggAAAACCGTCCTGAGCTGATGAGCGGCGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGGACTCTCCGGTTGCAGTTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCg < 2:769604/140‑1 (MQ=255) aaaaaCGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAac < 1:480804/143‑1 (MQ=255) aaaaaCGTCCTGACCTGATGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAac < 2:703571/143‑1 (MQ=255) tttctgccctgaTGACCGACGGTCCGCTGGAGTAGGTCGCGGCG‑GTCATGGCTGACGTTGCGAAATCCAGAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACaccacc < 2:218016/140‑1 (MQ=255) ctgacctgaTGATCGACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAAGCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCTTGTTCATCTTCCCCGATCTGAACACCGGTAACCCCCCCtcc > 2:565495/1‑141 (MQ=255) gACGGTCCGCTGGAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCAGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAAAACCGGTAACACCACCTACAAAGCGGTACAGCg > 1:187146/1‑143 (MQ=255) | TCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCG‑GTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCG > NC_000913/2416491‑2416752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |