BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,398	T→G	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	97.6%	95.7 / NA	42	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (17/24); total (17/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG  >  NC_000913/3041259‑3041533

tAGCTCTTTTACCATTAGTCATTAATACGTCGTTAAGCAACTCAGGACTCTATTAGGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGc                                                                                                                                      <  1:519696/143‑1 (MQ=255)

   ctctTTGACCATTAGTCATTAATACGCCGTTATGCAACTCAGTACTCTATTATGGCTTTTTCACCTTTTTCTTTCTTTCAGTCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccac                                                                                                                                   <  1:227812/143‑1 (MQ=255)

   cgcgtctACCACTAGACATTAACACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccac                                                                                                                                   <  2:744381/137‑1 (MQ=255)

                  tCATTAATACGCCGTTAAGCACCCCAGGACTCTATTATGGCTTTTACACCCTTTCCTCCCCGTCAGCCTACGGCTTCTGCTCGTTTACAGCTGACGCTGATGACGCGTGATGACTGGGCTCGTGCCACCATTACTGGCGCGGa                                                                                                                    <  2:253262/143‑1 (MQ=255)

                     ttAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTTATGACTTGCAGCGTGCCACCATTCCTGCCGCGAACAg                                                                                                                 >  1:763785/1‑143 (MQ=255)

                     ttAATACGCCGTTAAGCAACTCAGGACTCTAATATGGCTTTTACACCTTTTCCTCACCGTCAACCTACGGATTATACCCGTTTACCGATGACGATGATGACACTTGAGGACTGAGAGCGTGCAACAATTACAGGCAAGGACaa                                                                                                                 >  2:394305/1‑142 (MQ=255)

                            gCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCACGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCAATCCTGGCGCGCACAGCGaaaaa                                                                                                          >  2:406484/1‑143 (MQ=255)

                               gTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCTCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTGCTGGCGCGGACAGCGAAAAatat                                                                                                       <  1:649256/143‑1 (MQ=255)

                               gTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGCCGCTGATGACGCTTGCTGTCTGTGCGCGTTCCACCATAACTGACGCGGACAGCAAAAAatat                                                                                                       >  1:347691/1‑143 (MQ=255)

                                ttAAGCAACTCAGGTCTCTATTACGGCTTTTCCACCTTTTCCTCCCCGTCAGCCTACTGCTTCTGCCCGTTTTCCTCTTTTTCTGTTTATGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAatata                                                                                                      <  1:188597/143‑1 (MQ=255)

                                      aaCTCAGGACTCGATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGTCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTAGCTGACATGTCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAgg                                                                                                <  2:813323/143‑1 (MQ=255)

                                         tCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCACTACTGACGAGAACAGCGAAAAATATATGCAGGGTc                                                                                             >  1:750368/1‑143 (MQ=255)

                                               tcctATTATGGCTTTTTCGCCTTTTCTTCCCCGTCAGCCTACGGCTTCGTCCCGTTTACCGCTGACGCTGATGACGCTTGATTACTGGTCGCGTGCCACCATTACTGGCGCGTACAGTGAAAAATATATGCAGGGTCAGGTGa                                                                                       <  2:192460/141‑1 (MQ=255)

                                                 ctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGCCGCTCGTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGAGAAAAATATATGCAGGGTCAGGTGCca                                                                                     >  1:798606/1‑143 (MQ=255)

                                                  ttttatGGCTTTTGCGCCTTTTCCTCCCCGTCAGCCTTCGGCTTCTGCCCGGTTACCGCTGACGCTGATGACGCTGTATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcag                                                                                    <  2:222107/141‑1 (MQ=255)

                                                  tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCAGTTTACCGCTGACGCTGATGACGCGTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGGCAGCGAAAAAAGTAAGCAGGGTACGGTGAcag                                                                                    >  2:741564/1‑143 (MQ=255)

                                                          cTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTc                                                                            >  1:671397/1‑143 (MQ=255)

                                                           ttttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGCCGCTTGATGACTTGGCGCGTGCCACCATAACTGGCACGAACAGCAAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa                                                                           >  1:95580/1‑143 (MQ=255)

                                                           ctttACACCATCTACTCCCCGTCAGCCGACTGCCTCTGCCTGTTTGCGGCTGACGCAGACGACGCGTGATGACTGGGCGCGAGCCACCATCACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa                                                                           <  1:89083/142‑1 (MQ=255)

                                                                 acCTTCTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTCATGACTGGGCGCGTGCCACCATTACTTGCGCCGACAGCTAAAAATCTATGCAGGGTCAGGTGACAGCAGATGTCAGCCAgc                                                                     <  2:749249/143‑2 (MQ=255)

                                                                  ccTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACTCTGATGACGCTTGATGTCTGTGCGTTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAt                                                                    <  1:10180/143‑1 (MQ=255)

                                                                  ccTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCATGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGAGACAGGTGACAGAAGATGTCAACCAGag                                                                    >  2:192679/1‑142 (MQ=255)

                                                                     tttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGAGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATGTATGCAGGGTCAAGTGACAGCAGAGGGCAGCCAGATggg                                                                 >  2:219150/1‑142 (MQ=255)

                                                                      ttCCTCCCCGGCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGAGTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCa                                                                <  2:150304/143‑1 (MQ=255)

                                                                         ttccCCCTCAGCGTTCGGCTTCTGCCCGTTTGTCGCTGACGCTGATGACGATTGATGACGGGTCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGTTCAGGTGACAGCAGATGTCAGCCAGATGGCagaa                                                             <  2:314870/142‑1 (MQ=255)

                                                                         ctccCCGTCAGCCTACGGCTTCTGCCCGGTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCGTTACTGGCGCGGACGGCGCTAGATATATGCTGGCTTATGTGACAGAAAATGTCAGAAAGATGACagaa                                                             >  1:473760/1‑143 (MQ=255)

                                                                            cccGTCAGGCTAGCGCTTCTGTCCGTTCACCGCGGAGGCGGATGACGGCTCATGACTTGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGAt                                                          <  2:763785/143‑1 (MQ=255)

                                                                            cccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTCCTGGCGCGGACAGCTAAAAATATATACAAGATCAGGTGACAACAGATGTCAGACAGATGGCAGGAGAt                                                          >  1:307112/1‑143 (MQ=255)

                                                                                  aGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCAc                                                    <  2:415253/143‑1 (MQ=255)

                                                                                    ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGTTTGATGACTGTGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCt                                                  <  1:776694/143‑1 (MQ=255)

                                                                                             tgtGCCCGTTTACCGCTTCCTGTGGTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGAAAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgccgc                                         <  2:447904/141‑1 (MQ=255)

                                                                                                gCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCTCGGGCCACCATTACTGGCGCGGACAGCGAACAGTAGAGGTATGGTCAGGTGACCGCACGACACAGCGAGATGCCAGAAGGTGAGCACCGGCTCGCCGACCa                                      >  2:320102/1‑143 (MQ=255)

                                                                                                       tatcgctgacgctgaTGAGGTTTGATGACTGGGCGCGTGTCTCCATTTCTTTCGCGGACATCGAAAAAGAGATGCAGGGGCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGAc                               <  1:465963/140‑1 (MQ=255)

                                                                                                         ccGCTGGCGCTGATGACGCTGTATGACTGGGCCCGTGCCACCATTACTGGCGCGGACAGCGAAAGATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGc                             <  2:367613/143‑1 (MQ=255)

                                                                                                                 gctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGGTCAGCAACTGCTCGCCGCCCATTGCGCCGGCGAAGGTa                     >  2:762160/1‑143 (MQ=255)

                                                                                                                    gatgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGTCTTGTACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaaaa                  <  1:190057/143‑1 (MQ=255)

                                                                                                                      tgaCGCTTGATGACTGGGCGAGTGCCATCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACCGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg                <  2:141759/143‑1 (MQ=255)

                                                                                                                        gcGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtgtg              <  2:302766/142‑1 (MQ=255)

                                                                                                                         cGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTgg             <  2:103961/143‑1 (MQ=255)

                                                                                                                                 gaCTGGGCGCGTGCCACCATTTCTGGCGCGGACAGCGAAAATTATATGCATGTTCAGGTGTAAGCAGATGTCAGACAGATGGCAGAAGATCAGCACCTGCGCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAttt     <  1:45349/143‑1 (MQ=255)

                                                                                                                                 gaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAACATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGCAAAATGTGGAGCAAtgt     >  1:652624/1‑141 (MQ=255)

                                                                                                                                    tGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGAAAAACACCTACACGACGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACg  >  1:539226/1‑143 (MQ=255)

TAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACG  >  NC_000913/3041259‑3041533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`