Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 87,481 C→A T42K (ACA→AAA)  ilvH → acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091387,4810CA100.0% 56.0 / NA 28T42K (ACA→AAA) ilvHacetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/11);  total (17/11)

AGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAATCATG  >  NC_000913/87344‑87617
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agagaACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCTGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTAt                                                                                                                                     <  1:56613/143‑1 (MQ=255)
       cTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATGATCGCGTaa                                                                                                                              >  2:687359/1‑142 (MQ=255)
       cTGATTATGCGCCGGATATTATCAGACTTACTCGAAAATGAATCAGGCGCGTTATACCGAGGGATTGGCCTTTTTACCCAGCGAGGCTACAACATTGAAAGCCTGACCATTGCGCCAACCGACGAGCCGAAATTAAAGCGTAt                                                                                                                              >  2:223482/1‑143 (MQ=255)
          aTTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCAAACCGACGATCCGAAATTATCGCGTATGAc                                                                                                                           >  1:810108/1‑143 (MQ=255)
                cgcCGGATATTATAAGTCTTACTCGAAAATGAATCAGGCGCGTTATCACGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAACGGCTTACCTTTGGGCCACCCGACGATCCGAAATTATCGCGTATGACCATCCa                                                                                                                     <  1:299906/143‑1 (MQ=255)
                         ttatCATTCTTACTCGATAATTTATCAGGCGCGTTATTCGGTGTGATTGGGCTTTTTTCCCAGCGTGGCTACAACAGTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTggg                                                                                                            <  1:214404/143‑1 (MQ=255)
                         gtatCAGTGTTGCTCAAAAATGAATCCGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTCACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTggg                                                                                                            <  2:819338/142‑1 (MQ=255)
                              aGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTCCAACATTTGAAGCCTGTCCGTTGCGACAACAGAAGTTTCTTAATTAATTCATATGACAAGCCATACGGTGGGCGATg                                                                                                       >  1:553441/1‑143 (MQ=255)
                                  ttACTCGAAAATGAATCAAGCGCGTTATCCCGCGTGATTGGCCTTTTTTACCAGCGTGGCTACAACATTGAAAGCCTGACCATTGCGCCCACCGACGATACGAACTTATCGGAGATGACCAACCAGAACGTGGGCCATGaaaa                                                                                                   >  2:658648/1‑143 (MQ=255)
                                      tCGAATATGTGTCCGGCGGTTGATCCTGCGTGTGTGGCCTTTTTTCCCAGCGTAGCTACAACATTGAAATCCTGTCAGTTGCGCCAACCGATGATCCGAAATTATCGCGTATGACCATCCAGACCGTCGGCGATGAAAAAGTa                                                                                               <  2:145512/143‑1 (MQ=255)
                                        gAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTGCCTTCGTGGCTACAACATTGAAAGCCTGCCCGTTGCGCCAACCGTACATCCGAAATTACCGAGTATGCCCATCCTGACCGTGCGCCATGAACAAGTagc                                                                                             >  2:674303/1‑141 (MQ=255)
                                              gAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCACAGCGTGGCTACAACATTGAAAGCCTGACCGTTACGCCAACCGACGATCCGAAATTATCGCGTATGACCAACCAGCTCGTGGGCGATGACAAAATACTTGAGCa                                                                                       >  2:418696/1‑143 (MQ=255)
                                                  cAGGCGCGTTATCCCGCGTGATTGGCC‑TTTTTCCCAGCGTGGCGACAACATTGAAAGCCTGACCGTTGCGCCAACCTACGCTCCGTATTTATCGCGTTTGACTATCCAGACCGAGGAGGAAGAAAAACTATTTGAGCAgctgg                                                                                  >  1:736144/1‑139 (MQ=255)
                                                                cgcgTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGCCGATCCGTAATTATCGCGTATGACCATCCAGACAATGGACAAAGAAAAAGAAATTGAGCAGATAGAAAAACAATTaca                                                                     >  1:83031/1‑143 (MQ=255)
                                                                           ccTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTCTCACGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATAACAAAAACGGGGCg                                                          >  2:670725/1‑143 (MQ=255)
                                                                             ttttttCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGAAAAAAAAGTACTTGAGGAGATCGAAAAGCAATTACACAAAATGGACGAt                                                        >  1:712401/1‑143 (MQ=255)
                                                                             ttttttACCAGCGTGGCTACAACATGGAAAACCTGACCGTTGCGTCAACCGACGATGCGAAGAGATCGCGGATACCCATCAAGATCGCGGGCGATGAAAAAGTACTTGAAGAGAACGAAAGGCAATTACAAAAACTggaggct                                                        >  2:583062/1‑138 (MQ=255)
                                                                                    ccAGCGTGGCTACAACATTGAAGGCTGGACCGTTGCGCCAACCGACGAGCCGAAAGGATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTATTTGAGCAGATCGATAAGCAATTACACAAACTGGTCGATGTCTTgc                                                 <  2:315122/143‑1 (MQ=255)
                                                                                        tggagcgacaaccGTGAAAGCCTGACCGTTGCGCCACCCGACGATCCGAAATGATCGCGTATGACCATCCAGACCGTGGGCGATCAAAAAGTACTTGAGCAGATCGAAAAGCAATCACACAAACTTGTCGATGTCTTGCGCgt                                             <  2:294695/136‑1 (MQ=255)
                                                                                         gTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGAGGAAAAAGAAATTGAGAAGATAGAAAAGAAATAAAACAAACTAGTAGATGGATTGAGCgtg                                            >  1:33192/1‑143 (MQ=255)
                                                                                                caacaTTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGAATGACCATGCAGCATGAAGACGAAGAAAAAGTACTTGAGCCGAACGAAAAGCAAATAGAAAAACGGGGCAATGACTATCGCGAgagtgagt                                     >  2:482018/1‑143 (MQ=255)
                                                                                                        aaaGCCTGACCGTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGACCGAAAAGCAATTACACAAACTGGTCGAAGTCTTGCGCGTGAGTGAGTTGGGGCAg                             >  2:501292/1‑143 (MQ=255)
                                                                                                           gCCTGACCGTTGCGTCATCCTATGATTCGTAATTATCGCGTATGAATATCCAGTCCGTGTGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTTGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGgc                          <  2:716407/143‑1 (MQ=255)
                                                                                                                   gTTGCGCCAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACAGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTAAACAAAATGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGt                  >  1:795352/1‑143 (MQ=255)
                                                                                                                      gcgcCAACCGACGATCCGAAATTATCGCGTATGATCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGTAGATCGAAAAGCTATTACACAATCTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGa               <  2:672378/143‑1 (MQ=255)
                                                                                                                          cAACCGACGATCCGAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGGGAGTTGGAGCAGGGCGCGCATGTTGAGCgg           >  2:699046/1‑143 (MQ=255)
                                                                                                                                acgaTCCTAAATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAATc     <  2:714978/143‑1 (MQ=255)
                                                                                                                                   aTCCGAAATGACCGCGTATGACCAGCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAATCATg  <  1:578663/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
AGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCGCATGTTGAGCGGGAAATCATG  >  NC_000913/87344‑87617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑