| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 710,595 | G→T | P18T (CCT→ACT) | fur ← | DNA‑binding transcriptional dual regulator Fur |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 710,595 | 0 | G | T | 91.7% | 80.5 / NA | 37 | P18T (CCT→ACT) | fur | DNA‑binding transcriptional dual regulator Fur |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (19/14); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATA > NC_000913/710462‑710729 | naCTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCATACGTTTGTATAAATCTTCCGCACTGCCGTGAGGGTTGTCCGGCTCCTAAATAACGTCCCGGTTTCTTAAACGTGTAAGCATTAc > 2:421318/2‑143 (MQ=255)aaCTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTATTCACCCATATCGATCAGACGTTTGTATAAGTCTTCCGCACTGACGTGATGGTTGTGAGGCTCCTGAAGAAATTCCAGGATAGTTAAACGAGTAAGAGTTAc > 2:654929/1‑143 (MQ=255) tGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGTCGTTTGTATAAATCTTCCGCACTGTCGTGATGTTTGTCTGGCTCTTGAAGAACTTACAGAATTTTTAAACGAGTAAGCATTACttt > 1:113688/1‑143 (MQ=255) ggTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCCCCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTc > 1:647313/1‑143 (MQ=255) ggTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCTGCACTGACGTGATGGTTATCAGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACttta > 1:106453/1‑142 (MQ=255) gTTCAGTACGTGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCa < 1:586991/143‑1 (MQ=255) ttcgtttcgctATATATCGTAGTCATACCATTTTCTTCACCCATAACGATCAGACGATTGTATAAATCTGCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAg < 1:469517/136‑1 (MQ=255) cGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGATTGTATAAATCTTCCGCACTGACGTGATGGTTGTACGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCATGCAAGCTTTCTATAtgg > 2:675399/1‑143 (MQ=255) ccAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTa > 1:32625/1‑143 (MQ=255) gtccACTTTCTTCATCTATATGGATCAGTCGTTTCTATAAATCTTCCGTATTGACGTGTTGGTTGTCCGGCGCCAGAAGAACTGCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTg < 1:623371/141‑1 (MQ=255) aaTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGTCGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTAt < 2:141443/143‑1 (MQ=255) aTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGAAAGCGTTACTTTCAGACAAGCATTCTTTAGGGCGGTATTGTTATc > 1:725703/1‑143 (MQ=255) tttCTTCACCCATATCGCTCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCCCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCCGGCCAGCTTTCTTTAGGGCGGTATTGTTATCa < 2:438587/143‑1 (MQ=255) tatCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGATCTTCCAGGATTTTCAAACGAGTAAGCGTTTCTTTCGGGCAAGCTTTAGTTAGGGAGGTATTGTTATCAGTCATGAGGAAt > 1:463418/1‑143 (MQ=255) gACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTga > 1:399235/1‑142 (MQ=255) gACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGt < 1:773075/143‑1 (MQ=255) tCTTCCGCACTGACGGGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAATCGTTAATTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCa < 2:352942/143‑1 (MQ=255) ccGCAGTGACGTGATGTTTGTCCTGCTACTGAATAACTTCCATGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGTGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTc < 2:700913/143‑1 (MQ=255) cACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCAtt < 2:32625/143‑1 (MQ=255) gATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCCGGCCAGCTTTCTTTTGGCCGGTCTTCTTATCAGTCATAAGAAATATAACCAATAACTAAACGGTTCACTTCATTAGAAGAAGt > 1:653954/1‑143 (MQ=255) gATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGt > 1:652348/1‑143 (MQ=255) gATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCAAACGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGt > 1:555207/1‑143 (MQ=255) ggTTGTCCGGCTCCTGTAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACAAAGCGGTTCACTTCATTAGAAGAAGTGAc > 2:509267/1‑143 (MQ=255) gTCCGTCTCTTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGaa < 2:833071/143‑1 (MQ=255) tCCGGCTCCTGAAGAACTTCTAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAt < 1:596442/143‑1 (MQ=255) gCTCCTGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTCCTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGc > 2:156709/1‑143 (MQ=255) tCCTGAAGATTTTTCAGGATTTTTAAACGAGTATGCGTTACTTTCAGGCTAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGctc < 1:430549/143‑1 (MQ=255) tGAAGAACTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCAGTAGAAGAAGTGACAGAATTTGCTCTTg > 2:529717/1‑143 (MQ=255) tgagtaCTTCCAGGATTTTTTAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTga < 2:28760/138‑1 (MQ=255) gaataaCTTCCATGATTTTTAAACGAGTAAGCGTTACTTTCAGGTCAGCTTTCTTTAGGGCTGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTTATTAGAAGAAGTGACAGAATTTGCTCTTga < 2:113688/143‑1 (MQ=255) gaagaaCTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGCATTTGCTCTTga > 1:471632/1‑143 (MQ=255) agaaCTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCAGTAGTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTgaga > 1:743974/1‑143 (MQ=255) gaaCTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGAt > 2:602871/1‑143 (MQ=255) gaaCTTCCAGGATTTTTAAACGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGAt > 1:49119/1‑143 (MQ=255) aGGATTTTTAAGCGAGTATGCTTTACTTTCAGGTCAGTTTTCTTTAGTGCGTTTTTTTTATCAGTCATTCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTa < 1:678809/143‑1 (MQ=255) ttAAGCGAGTAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTGTCATtat > 2:209101/1‑143 (MQ=255) tAAACGAGGAAGGTTTGCTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATtata < 2:472152/143‑1 (MQ=255) | AACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATA > NC_000913/710462‑710729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |