Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,220	C→T	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,220	0	C	T	96.3%	54.6 / NA	28	H366H (CAC→CAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (13/13);  minor base G (1/0);  total (15/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGA  >  NC_000913/1196082‑1196315                                                                                                                                           |                                                                                                cggtatcgTCCCTGTTGCAATCATCGGTGATGAATGCGCCCTGTCTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAACGTAAATCCTGGTTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATgg                                                                                             <  2:18177/143‑1 (MQ=255) cggtagcgCCCCTGGTGCAAACATCGGTGACTATTGCGCCCTGTTTGTAGCCATTCTTGGTACTGTGCAGAAATAGGCAGGGCAGGACAAAGTAAAGCCTGGCGCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATgg                                                                                             <  1:266343/143‑1 (MQ=255)   gtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAATAAATCAAGGCACAATTATTCTATCCGCTGAGATGATGCTGCGCCAGATGGGt                                                                                           >  1:247706/1‑143 (MQ=255)        gCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGAc                                                                                      >  1:775071/1‑143 (MQ=255)           ccTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACTCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGa                                                                                   <  1:745451/143‑1 (MQ=255)               gTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCCCCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTGAATCCTGGCTCTATTATTCCCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCg                                                                               <  2:637375/143‑1 (MQ=255)                   aaaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTg                                                                           <  1:213542/143‑1 (MQ=255)                           gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAGAGTAAATCCTGGCTCTATTATTCTCTACGATGAGATGAAGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgata                                                                   >  1:98943/1‑137 (MQ=38)                                 aagtcgcCCTGTTTGAAGACACTTACGGTACTGCGCCGAAATATGCCGCTCAGGACAATGTAAATCCTTTCTCTTTTATTCTCTCCGCTGAGATGCTTCTGCGCCATATGGGTTTGACTGAAGCGGTTGATCTGATTGTTaaa                                                             <  2:465127/139‑1 (MQ=255)                                 aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                             <  1:727946/143‑1 (MQ=38)                                     cgcCCGGTGTGGAGCCACCCACGGTACTGCGGCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa                                                         <  2:593598/143‑1 (MQ=25)                                      ggccTGTGTGCAGCCAACCACGGTACAGCGCCGAAATATGCCGGTCAGGGCAAAGTAAATCCTGGCTCTATAATTCTTTCCGCGGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                                        <  2:713344/141‑1 (MQ=21)                                      gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAACTGATTGTTAAAGGTAt                                                        >  2:339431/1‑143 (MQ=38)                                        ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTACACCATATGAGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg                                                      >  1:286419/1‑143 (MQ=37)                                         cTGTTTGAAGCCACCCACGGTATTTGCCCTAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTTAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTTATTGTTAAAGGTATGGa                                                     <  2:177607/143‑1 (MQ=25)                                            tttGAAGCCACCCACGTTACTGCGCCGAAATATGCCGGTCAGGATAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGAAGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg                                                  <  1:675436/143‑1 (MQ=25)                                                   ccacccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATATGGGTTGGACTGTATCGGCTGATCTGATTGTTAAAGGTATGAAAGCCGCAATc                                           >  1:113098/1‑143 (MQ=25)                                                        cccGGTACTGCGCCGAAATTTGGCGGTTAGTAGAAAGTTAATTCGGGCTCTATTATTCTCTCCGCTGAGATGATGATGCGCCATATGGGTTGTACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc                                      <  2:539175/141‑1 (MQ=17)                                                        cacGGTACTGCGCCATAATACGCCGGTCAGGACAAAGTAACTCCTGGCTCTATTATTCGCTCCGCTGATATGATGTTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc                                      <  2:403547/143‑1 (MQ=18)                                                                  cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCTAAACGTTTCTAACTTAAGCGGCTGACCTGATTGTTAAAGGAATGGAAGGCGCAATCAATGCCAAGAGCGTa                            >  1:257901/1‑143 (MQ=18)                                                                  cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCCTATGGGTTGGACTGAAGCGGCTGACATGAATGATAAAGATATAGAAGGCGCAATCAATGCCAAGACCGTa                            >  1:379915/1‑143 (MQ=21)                                                                       aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAActta                       >  1:14311/1‑140 (MQ=17)                                                                       aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATCCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGACGCGGATGACCTGATTGTTAAAAGTATGGAAGGCGCAATAAAAGCCTACACCGTCACCTa                       >  2:488971/1‑143 (MQ=18)                                                                            tGCTGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGCTGCTGCGCCATATGGGTTGGCCTGAAGCGGCTGACCTGATCGTTAAAGGTATGGAAGGCGCAAACAATGCCAAGACCGTAACATATGAca                  >  1:531379/1‑142 (MQ=17)                                                                            tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCAGACATAACTGCCTAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt                  >  2:353225/1‑143 (MQ=18)                                                                              ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAAGCGCAATCAATGCCAAGACCGTAACTTATGACTTc                >  1:665817/1‑143 (MQ=17)                                                                                      gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt        >  2:227623/1‑143 (MQ=14)                                                                                            gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGACATATGGGTTGGAGTGAAGCAGATGACATGATTGATAAAGGTATGGAAGGAGAAATAAATGCAAAAACCGTAACTTATGACTTCGAACGTATgatgga  >  1:229466/1‑143 (MQ=14)                                                                                                                                           |                                                                                                CGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGA  >  NC_000913/1196082‑1196315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑