Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927371,5610TG25.9% 315.5 / 15.9 116N136T (AAC→ACC) thiBthiamine‑, thiamine pyrophosphate‑, thiamine monophosphate‑binding ABC transporter periplasmic protein; ThiBPQ thiamine, thiamine pyrophosphate importer
Reads supporting (aligned to +/- strand):  ref base T (14/72);  new base G (30/0);  total (44/72)
Fisher's exact test for biased strand distribution p-value = 2.18e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 144 total aligned reads displayed.

AGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCG  >  CP009273/71462‑71660
                                                                                                   |                                                                                                   
aGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAAACACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGtt                                                                                                    <  2:3018207/101‑1 (MQ=255)
  cAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGtttt                                                                                                  <  2:1462676/101‑1 (MQ=255)
  cAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGtttt                                                                                                  <  1:2019980/101‑1 (MQ=255)
    aCAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTc                                                                                                >  1:2387587/1‑101 (MQ=255)
     cAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGgttttta                                                                                               >  1:2776612/1‑99 (MQ=255)
     cAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCa                                                                                               <  2:1403818/101‑1 (MQ=255)
      aGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGgtttttat                                                                                              >  1:306822/1‑98 (MQ=255)
          ccAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTg                                                                                          <  2:302731/101‑1 (MQ=255)
            aGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGtt                                                                                        <  1:485031/101‑1 (MQ=255)
            aGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTGtt                                                                                        >  1:790885/1‑101 (MQ=255)
            aGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTCTATTTGtt                                                                                        >  2:2527045/1‑101 (MQ=255)
             gCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTc                                                                                       <  1:1093852/101‑1 (MQ=255)
              ccccGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTGtttt                                                                                      >  2:477846/1‑99 (MQ=255)
              ccccGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGCTTTTCAAtttttttt                                                                                      >  2:1608815/1‑99 (MQ=255)
               cccGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGATTTTCAATTTGTTCtt                                                                                     >  1:2713575/1‑101 (MQ=255)
                ccGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTg                                                                                    <  2:1262679/101‑1 (MQ=255)
                   gtgtACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCa                                                                                 <  1:706105/101‑1 (MQ=255)
                    tgtACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCAt                                                                                <  2:1803511/101‑1 (MQ=255)
                    tgtACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTTTTGTTTTCAt                                                                                >  2:196306/1‑101 (MQ=255)
                     gtACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATa                                                                               <  1:2951996/101‑1 (MQ=255)
                     gtACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTTTTTTTGTCATa                                                                               >  1:2757939/1‑101 (MQ=255)
                     gtACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTGTTCTTTTCATa                                                                               >  1:2313365/1‑101 (MQ=255)
                      tACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATaa                                                                              <  2:1371837/101‑1 (MQ=255)
                       aCTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATaaa                                                                             <  2:3085973/101‑1 (MQ=255)
                        cTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAAc                                                                            <  2:1863031/101‑1 (MQ=255)
                        cTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAAc                                                                            <  2:1234530/101‑1 (MQ=255)
                         tGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTGTTTTTTTCATaaaag                                                                           >  2:1178547/1‑99 (MQ=255)
                         tGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTCTTTTTGTTTTTGTCCTaaaag                                                                           >  2:1363302/1‑99 (MQ=255)
                           gTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGaa                                                                         <  1:280302/101‑1 (MQ=255)
                           gTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGaa                                                                         <  1:2916223/101‑1 (MQ=255)
                            tGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTTAGTTTGTTCTTGTCATAAACGAAg                                                                        >  1:2637212/1‑101 (MQ=255)
                            tGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAg                                                                        <  2:1798723/101‑1 (MQ=255)
                             gcgcGGATCCTGATAAATCTCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAgg                                                                       <  2:1496720/101‑1 (MQ=255)
                             gcgcGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAgg                                                                       <  2:2504134/101‑1 (MQ=255)
                             gcgcGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGCTTTTTAGTTTTTTTTTTTCCTAAAAGAAgg                                                                       >  1:986294/1‑101 (MQ=255)
                              cgcgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGc                                                                      <  1:1604505/101‑1 (MQ=255)
                              cgcgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTTTCCTAAACaaaagg                                                                      >  2:294425/1‑98 (MQ=255)
                                cgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTTTTCTTGTCCTAAACGAAGGCGa                                                                    >  1:393633/1‑101 (MQ=255)
                                 ggATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGaa                                                                   >  1:2214423/1‑101 (MQ=255)
                                 ggATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTTTTTGTTTTTTTTATAAAAAAAGGCGaa                                                                   >  2:2914635/1‑101 (MQ=255)
                                  gATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTCAGTTTGTTCTTTTCATAAAAGAAGGGGAAg                                                                  >  1:2381820/1‑101 (MQ=255)
                                    tCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTa                                                                <  1:1589450/101‑1 (MQ=255)
                                     ccTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGCTTTTCAGTTTTTTTTTGTCCTAAACGAAGGCGAAGTAg                                                               >  1:972692/1‑101 (MQ=255)
                                         aTAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCAt                                                           <  2:650290/101‑1 (MQ=255)
                                         aTAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGGAGccct                                                           >  2:2158160/1‑99 (MQ=255)
                                          tAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTGTTCTTGTCATAAACGAAAGCGAAATAACCATa                                                          >  1:1026347/1‑101 (MQ=255)
                                           aaaTCTCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATaa                                                         <  1:1334957/101‑1 (MQ=255)
                                           aaaTCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATaa                                                         <  1:2854673/101‑1 (MQ=255)
                                           aaaTCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTTTTCTTTTTCTAAACCAAGGCGAAGGAGCCATaa                                                         >  1:1422881/1‑101 (MQ=255)
                                            aaTCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAAt                                                        <  1:3203185/101‑1 (MQ=255)
                                             accACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATc                                                       <  2:2148459/99‑1 (MQ=255)
                                                aCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTTATTTTTTTCTTGTCCTAAACGAAGGCGGAGAAGCCATAATCaaa                                                    >  1:1081480/1‑101 (MQ=255)
                                                aCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAATTTTTTTTTGTCATAAACAAAGGGGAAGGAGCCCTAATCaaa                                                    >  2:1428220/1‑101 (MQ=255)
                                                 cccGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGTTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAAc                                                   <  1:3316533/101‑1 (MQ=255)
                                                  ccGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACg                                                  <  1:2154381/101‑1 (MQ=255)
                                                   cGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACgg                                                 <  2:2624853/101‑1 (MQ=255)
                                                   cGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTCAGTTTTTTCTTGTCATAAAAAAAGGCGAAGTAGCCCTAATCAAACgg                                                 >  2:3193078/1‑101 (MQ=255)
                                                    gCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGt                                                >  1:2965387/1‑101 (MQ=255)
                                                     ccAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTa                                               <  1:2563391/101‑1 (MQ=255)
                                                      cAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTAc                                              <  1:1214428/101‑1 (MQ=255)
                                                      cAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGCTTTTTAGTTTTTTTTTGTCTTAAAAGAAAAAGAAGAACCCACAATCAAACAGttc                                              >  2:496603/1‑99 (MQ=255)
                                                       aGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTTTGTTTGTTTTTGTCATAAAAGAAGGCGAAGTAGCCATAATCAAACGGTACg                                             >  1:357642/1‑101 (MQ=255)
                                                        gTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTTAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGa                                            >  1:308372/1‑101 (MQ=255)
                                                        gTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGa                                            <  1:3274295/101‑1 (MQ=255)
                                                        gTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTGTTCTTGTCATAAACGAAGGGGAAGGAGCCATAATCAAACGGGACGa                                            >  1:1369186/1‑101 (MQ=255)
                                                          tttGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTGTTCTTTTTCTAAACAAAGGGGAAGAAGACATAATCAAACGGTAAGaaa                                          >  2:2470468/1‑101 (MQ=255)
                                                          tttGATCGCTCTCAACCAGTTCTTTCAGGCTTTGGGGCGGTTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGaaa                                          <  2:2869117/101‑1 (MQ=255)
                                                           ttGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAg                                         <  2:1189458/101‑1 (MQ=255)
                                                           ttGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTTTTTTTTTCATAAAAGAAGGCGAAGGAGGCATAATCAAAAGGGACGGAAg                                         >  2:2396926/1‑101 (MQ=255)
                                                               tCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTTTTTTTTTTATAAAAAAAAGAGAAGGAGGCATAATCAAAAGGGACGAAAgaggg                                     >  2:54793/1‑97 (MQ=255)
                                                                cGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCa                                    <  1:1701669/101‑1 (MQ=255)
                                                                cGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTTTTTTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCa                                    >  1:1876406/1‑101 (MQ=255)
                                                                   tctcAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTCGTTTTTTTTTTTCATAAAAGAAGGCGAAGGAGCCATAATCAAACGGTACGAAAGAGTCatta                                 >  2:1207503/1‑101 (MQ=255)
                                                                    ctcAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCattat                                <  2:1525517/101‑1 (MQ=255)
                                                                    ctcAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCattat                                <  2:270741/101‑1 (MQ=255)
                                                                        aCCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCa                            <  1:559892/101‑1 (MQ=255)
                                                                        aCCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCa                            <  2:2156897/101‑1 (MQ=255)
                                                                        aCCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTTTCTTAAAAAAAAGGGAAAAAGCCATAATAAAACAGGAAGAAAGAGGCATTATTccc                            >  2:2596781/1‑100 (MQ=255)
                                                                          cAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAgc                          <  1:62228/101‑1 (MQ=255)
                                                                          cAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTTTTTTTTTCATAAAAGAAAGCGAAGTAGCCATAATCAAAAGGGACGAAAGTGTCATTATTTCAgc                          >  2:2429296/1‑101 (MQ=255)
                                                                           aGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAgcc                         <  2:2719493/101‑1 (MQ=255)
                                                                            gTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAgccg                        <  1:707605/101‑1 (MQ=255)
                                                                            gTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAgccg                        <  2:2090018/101‑1 (MQ=255)
                                                                             ttCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAgccgc                       <  1:2710554/101‑1 (MQ=255)
                                                                              tCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTTCAgccgcc                      >  1:801536/1‑101 (MQ=255)
                                                                               cTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGccgccg                     <  1:1897775/101‑1 (MQ=255)
                                                                                 ttCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTTTTTTAAACCAAGGCGAAGGAACCCAAAAAAAACGGAAAGAAAGTAATATTATTTCAGCCGCCcgg                   >  2:49285/1‑101 (MQ=255)
                                                                                  tCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGa                  >  1:1984430/1‑101 (MQ=255)
                                                                                  tCAGGCTTTGTGGCGGGGTTTTTAGTTTTTTTTTTTTATAAAAAAAAGCGAAGAAGCCATAAACAAAAAGGACGAAAAAGTCATTATTTCAGCCGCCgggg                  >  2:1691264/1‑100 (MQ=255)
                                                                                   cAGGCTTTGTGGCGGGGTTTTTTGTTTGTTCTTGTTATAAAAGAAGGGGAAGGAGCCATAAATAAACGGGACGAAAGGGTGTTTATTCCAGCCGCCGGGaa                 >  2:809378/1‑101 (MQ=255)
                                                                                     ggCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACg               <  1:587421/101‑1 (MQ=255)
                                                                                      gCTTTGTGGCGGTTTTTTCAGTTTTTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGt              <  2:590998/101‑1 (MQ=255)
                                                                                       cTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGtt             <  2:3278168/101‑1 (MQ=255)
                                                                                       cTTTGTGGCGGGGTTTTTAGTTTGTTTTTGTTATAAAAAAAAGCGAAGGAGCCAAAATCAAACGGTACGAAAGAGTCATTATTTCCGCCGCCGGGGACggt             >  2:1946374/1‑99 (MQ=255)
                                                                                        tttGTGGCGGGTTTTTCATTTTGTTCTTGTCATAAACGAAGGCAAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTa            <  2:913489/101‑1 (MQ=255)
                                                                                           ttgGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACg         <  2:3257177/100‑1 (MQ=255)
                                                                                           gtgGCGGGTTTTTCAGTTTGTTCTTGGTTTAAACCAAGGCGAAGGCACCCTAATCAAAAAGTACGGAAGCGGCAATATTACAGCCGCCCGGGACGGTAAca         >  2:1937474/1‑100 (MQ=255)
                                                                                            tgGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGGACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACgg        >  2:1857227/1‑101 (MQ=255)
                                                                                             ggCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGc       <  2:790885/101‑1 (MQ=255)
                                                                                                  gTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCg  <  1:772560/101‑1 (MQ=255)
                                                                                                   |                                                                                                   
AGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCG  >  CP009273/71462‑71660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑