BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,528,814	G→A	D464N (GAC→AAC)	ptsI →	PEP‑protein phosphotransferase of PTS system (enzyme I)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,528,814	0	G	A	100.0%	529.6 / NA	128	D464N (GAC→AAC)	ptsI	PEP‑protein phosphotransferase of PTS system (enzyme I)
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (68/60); total (68/60)

Only 100 of 128 total aligned reads displayed.

AAACACCGGCTGCCGCAACAATTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTGACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTTCTCATGCTGAAG  >  CP009273/2528716‑2528910

aaaCACCGGCTGCCGCAACAATTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAAc                                                                                                >  1:2966248/1‑101 (MQ=255)

 aaCACCGGCTGCCGCAACAATTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAAcc                                                                                               <  2:1926683/101‑1 (MQ=255)

     ccGGCTGCCGCAACAATTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTgg                                                                                           >  1:2790755/1‑101 (MQ=255)

      cGGCTGCCGCAACAATTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGt                                                                                          >  2:2508080/1‑101 (MQ=255)

        gCTGCCGCAACAATTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTaa                                                                                        <  2:1558334/101‑1 (MQ=255)

          tGCCGCAACAATTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAatg                                                                                      >  2:2801507/1‑101 (MQ=255)

          tGCCGCAACAATTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAatg                                                                                      >  2:2324870/1‑101 (MQ=255)

           gCCGCAACAATTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAatga                                                                                     >  1:3191284/1‑101 (MQ=255)

           gCCGCAACAATTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAatga                                                                                     >  1:78000/1‑101 (MQ=255)

              gCAACAATTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAatgatat                                                                                  >  2:3168501/1‑101 (MQ=255)

                aacaaTTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAatgatatga                                                                                >  1:856174/1‑101 (MQ=255)

                 acaaTTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAatgatatgat                                                                               <  1:309456/101‑1 (MQ=255)

                 acaaTTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAatgatatgat                                                                               >  2:533085/1‑101 (MQ=255)

                  caaTTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGAtt                                                                              >  1:49158/1‑101 (MQ=255)

                    aTTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTc                                                                            >  2:1313423/1‑101 (MQ=255)

                     ttGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTca                                                                           <  2:2311002/101‑1 (MQ=255)

                     ttGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTca                                                                           <  2:2367241/101‑1 (MQ=255)

                      tGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTcac                                                                          <  1:2051723/101‑1 (MQ=255)

                           gTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCtt                                                                     >  1:48626/1‑101 (MQ=255)

                            tCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCttt                                                                    >  1:214518/1‑101 (MQ=255)

                            tCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCttt                                                                    <  1:1102436/101‑1 (MQ=255)

                             cATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTa                                                                   <  1:1527704/101‑1 (MQ=255)

                              aTTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGGTTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTAc                                                                  <  1:3263721/101‑1 (MQ=255)

                              aTTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTAc                                                                  >  1:1782563/1‑101 (MQ=255)

                               tttAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTAcc                                                                 <  1:2558484/101‑1 (MQ=255)

                                ttAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCa                                                                <  2:2921644/101‑1 (MQ=255)

                                ttAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCa                                                                >  2:45835/1‑101 (MQ=255)

                                 tAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAg                                                               >  1:2803103/1‑101 (MQ=255)

                                 tAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAg                                                               >  2:773066/1‑101 (MQ=255)

                                  aGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGc                                                              >  1:954235/1‑101 (MQ=255)

                                   gCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGcc                                                             <  1:2162340/101‑1 (MQ=255)

                                   gCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGcc                                                             <  2:3239797/101‑1 (MQ=255)

                                     cAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCaa                                                           >  1:1507370/1‑101 (MQ=255)

                                     cAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCaa                                                           >  2:3168625/1‑101 (MQ=255)

                                     cAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCaa                                                           >  2:1536529/1‑101 (MQ=255)

                                      aaaGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAAt                                                          >  1:1902797/1‑101 (MQ=255)

                                        agaagTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGt                                                        <  2:2252502/101‑1 (MQ=255)

                                        agaagTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGt                                                        >  1:1420661/1‑101 (MQ=255)

                                          aagTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACCCTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCa                                                      <  2:339727/101‑1 (MQ=255)

                                           agTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAc                                                     >  1:2481569/1‑101 (MQ=255)

                                             ttGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAccg                                                   <  1:242704/101‑1 (MQ=255)

                                              tGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAccgt                                                  >  2:1587911/1‑101 (MQ=255)

                                                aTTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAccgtcc                                                <  1:2014143/101‑1 (MQ=255)

                                                aTTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAccgtcc                                                <  1:1494862/101‑1 (MQ=255)

                                                aTTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAccgtcc                                                >  2:3102230/1‑101 (MQ=255)

                                                 tttCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAccgtccg                                               <  2:3264285/101‑1 (MQ=255)

                                                 tttCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAccgtccg                                               <  2:3005081/101‑1 (MQ=255)

                                                 tttCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAccgtccg                                               >  2:739333/1‑101 (MQ=255)

                                                 tttCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAccgtccg                                               >  1:756507/1‑101 (MQ=255)

                                                  ttCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAccgtccgt                                              >  1:1151102/1‑101 (MQ=255)

                                                  ttCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAccgtccgt                                              <  2:2625409/101‑1 (MQ=255)

                                                  ttCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCAccgtccgt                                              >  2:1007481/1‑101 (MQ=255)

                                                   tCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTg                                             >  2:691369/1‑101 (MQ=255)

                                                      ttAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTg                                          <  2:534790/101‑1 (MQ=255)

                                                      ttAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTg                                          >  1:2403371/1‑101 (MQ=255)

                                                      ttAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTg                                          >  2:1910742/1‑101 (MQ=255)

                                                       tAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGa                                         <  1:2508080/101‑1 (MQ=255)

                                                         gTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAAc                                       <  2:2774255/101‑1 (MQ=255)

                                                         gTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAAc                                       <  2:1733967/101‑1 (MQ=255)

                                                          tATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACt                                      <  1:1193487/101‑1 (MQ=255)

                                                           aTCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACtt                                     <  2:1256133/101‑1 (MQ=255)

                                                           aTCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACtt                                     <  2:572260/101‑1 (MQ=255)

                                                           aTCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACtt                                     >  1:1626099/1‑101 (MQ=255)

                                                           aTCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACtt                                     >  1:1972236/1‑101 (MQ=255)

                                                           aTCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACtt                                     >  2:2494370/1‑101 (MQ=255)

                                                           aTCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACtt                                     <  1:2781740/101‑1 (MQ=255)

                                                              ggCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGAt                                  <  2:49158/101‑1 (MQ=255)

                                                              ggCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGAt                                  <  1:2630662/101‑1 (MQ=255)

                                                              ggCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGAt                                  >  2:2967162/1‑101 (MQ=255)

                                                               gCACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATc                                 <  1:615567/101‑1 (MQ=255)

                                                                cACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATca                                >  1:1362723/1‑101 (MQ=255)

                                                                cACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATca                                >  1:2931383/1‑101 (MQ=255)

                                                                cACCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATca                                >  2:493811/1‑101 (MQ=255)

                                                                 aCCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATcaa                               <  1:2157013/101‑1 (MQ=255)

                                                                 aCCAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATcaa                               >  1:121287/1‑101 (MQ=255)

                                                                  ccAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATcaag                              >  1:607846/1‑101 (MQ=255)

                                                                  ccAATGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATcaag                              >  1:2038664/1‑101 (MQ=255)

                                                                    aaTGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATcaagca                            >  1:586817/1‑101 (MQ=255)

                                                                      tGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATcaagcaag                          >  1:563278/1‑101 (MQ=255)

                                                                      tGATTTAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATcaagcaag                          <  1:161244/101‑1 (MQ=255)

                                                                       gATTTAACGCAGTACACTCTGGGAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCGAGCAAGt                         >  1:2818897/1‑101 (MQ=255)

                                                                         tttAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTa                       >  2:2766006/1‑101 (MQ=255)

                                                                         tttAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTa                       <  1:2414633/101‑1 (MQ=255)

                                                                           tAACGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTAtt                     <  1:976072/101‑1 (MQ=255)

                                                                            aaCGCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTg                    >  1:177185/1‑101 (MQ=255)

                                                                               gCAGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATg                 >  1:2854434/1‑101 (MQ=255)

                                                                                 aGTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCt               <  1:790332/101‑1 (MQ=255)

                                                                                  gTACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCtt              <  2:2153168/101‑1 (MQ=255)

                                                                                   tACACTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTtc             >  1:2089342/1‑101 (MQ=255)

                                                                                    acacTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTtct            >  2:1350991/1‑101 (MQ=255)

                                                                                    acacTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTtct            >  1:359281/1‑101 (MQ=255)

                                                                                     cacTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTtctc           >  1:2692955/1‑101 (MQ=255)

                                                                                      acTCTGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTTCTCa          <  1:945149/101‑1 (MQ=255)

                                                                                        tctGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTTCTCATg        <  1:2581268/101‑1 (MQ=255)

                                                                                          tGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTTCTCATGCt      >  2:3329043/1‑101 (MQ=255)

                                                                                          tGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTTCTCATGCt      <  2:277212/101‑1 (MQ=255)

                                                                                          tGGCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTTCTCATGCt      >  2:537762/1‑101 (MQ=255)

                                                                                            gCAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTTCTCATGCTGa    >  1:3282587/1‑101 (MQ=255)

                                                                                             cAGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTTCTCATGCTGaa   >  2:2008753/1‑101 (MQ=255)

                                                                                              aGTTAACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTTCTCATGCTGAAg  <  1:559656/101‑1 (MQ=255)

AAACACCGGCTGCCGCAACAATTGCACGTCATTTAGCCAAAGAAGTTGATTTCTTTAGTATCGGCACCAATGATTTAACGCAGTACACTCTGGCAGTTGACCGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTGATCAAGCAAGTTATTGATGCTTCTCATGCTGAAG  >  CP009273/2528716‑2528910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`