Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 132,999 A→G intergenic (‑48/+58) speE ← / ← yacC spermidine synthase (putrescine aminopropyltransferase)/PulS_OutS family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273132,9990AG100.0% 612.5 / NA 152intergenic (‑48/+58)speE/yacCspermidine synthase (putrescine aminopropyltransferase)/PulS_OutS family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (74/78);  total (74/78)

Only 100 of 155 total aligned reads displayed.

ACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGG  >  CP009273/132876‑133121
                                                                                                                            |                                                                                                                          
aCAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACatgat                                                                                                                          <  2:585828/126‑1 (MQ=255)
 cAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACatgata                                                                                                                         <  2:1349565/126‑1 (MQ=255)
 cAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACatgata                                                                                                                         <  1:839175/126‑1 (MQ=255)
      cGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTaa                                                                                                                    <  1:1002385/126‑1 (MQ=255)
         tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACAt                                                                                                                 >  1:1704115/1‑126 (MQ=255)
          aTCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACAtt                                                                                                                <  2:1775666/126‑1 (MQ=255)
            cTACCCCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTg                                                                                                               <  2:1943166/126‑1 (MQ=255)
             tACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGAc                                                                                                             >  1:3236365/1‑126 (MQ=255)
              aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGAcc                                                                                                            <  2:574158/126‑1 (MQ=255)
              aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGAcc                                                                                                            >  2:1149322/1‑126 (MQ=255)
               ccGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACcg                                                                                                           <  2:2377376/126‑1 (MQ=255)
                cGCAAAGTACTGCCCAGACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACcgc                                                                                                          >  2:3191253/1‑126 (MQ=255)
                cGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACcgc                                                                                                          <  2:406492/126‑1 (MQ=255)
                   aaaGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGa                                                                                                       >  2:1705768/1‑126 (MQ=255)
                    aaGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGAt                                                                                                      <  1:2184646/126‑1 (MQ=255)
                       tACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCa                                                                                                   <  2:2124438/126‑1 (MQ=255)
                         cTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACg                                                                                                 <  2:2619224/126‑1 (MQ=255)
                          tGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACgg                                                                                                >  1:603758/1‑126 (MQ=255)
                          tGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACgg                                                                                                <  1:711937/126‑1 (MQ=255)
                            cccAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTc                                                                                              <  2:2497798/126‑1 (MQ=255)
                            cccAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTc                                                                                              <  1:1590658/126‑1 (MQ=255)
                              cAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCaa                                                                                            >  1:1232163/1‑126 (MQ=255)
                               aaaCTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACATCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATCCACGGTCAat                                                                                           >  2:2858293/1‑126 (MQ=255)
                                aaCTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAata                                                                                          <  1:1307986/126‑1 (MQ=255)
                                aaCTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACAAGATAGCTAACATTGACCGCGGATGCACGGTCAata                                                                                          >  2:809486/1‑126 (MQ=255)
                                 aCTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGTGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAatat                                                                                         >  1:1874765/1‑126 (MQ=255)
                                  cTGGTCGTGTAGCGTGTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATAtt                                                                                        <  2:2706716/126‑1 (MQ=255)
                                   tGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATAttt                                                                                       <  1:2304168/126‑1 (MQ=255)
                                     gTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCt                                                                                     <  2:3391163/126‑1 (MQ=255)
                                     gTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCt                                                                                     >  1:918116/1‑126 (MQ=255)
                                        gtgtAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACa                                                                                  >  1:38279/1‑126 (MQ=255)
                                         tgtAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑AAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaaa                                                                                >  1:3392011/1‑126 (MQ=255)
                                            aGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaaaaa                                                                              <  2:2572514/126‑1 (MQ=255)
                                            aGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaaaaa                                                                              >  2:1189480/1‑126 (MQ=255)
                                              cGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAgg                                                                            >  2:146064/1‑126 (MQ=255)
                                               gTTTCATGCCGCTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGt                                                                           >  1:3092823/1‑126 (MQ=255)
                                               gTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGt                                                                           >  1:2998477/1‑126 (MQ=255)
                                                 ttCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTAt                                                                         >  1:147631/1‑126 (MQ=255)
                                                 ttCATGCCACTG‑TTTTTTTTCGGACATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTAt                                                                         >  1:3154964/1‑126 (MQ=255)
                                                  tCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATc                                                                        >  1:1366874/1‑126 (MQ=255)
                                                  tCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATc                                                                        <  1:2095936/126‑1 (MQ=255)
                                                   cATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCa                                                                       >  2:204500/1‑126 (MQ=255)
                                                    aTGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAg                                                                      <  2:2292053/126‑1 (MQ=255)
                                                      gCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACAAGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAggg                                                                    <  1:809486/126‑1 (MQ=255)
                                                        cACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCAGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGat                                                                  <  2:1544012/126‑1 (MQ=255)
                                                          cTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGatta                                                                <  2:3364592/126‑1 (MQ=255)
                                                           tG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattat                                                               >  1:814301/1‑126 (MQ=255)
                                                            g‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt                                                              <  2:2904099/126‑1 (MQ=255)
                                                              ttttttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTAttt                                                             <  1:3284921/126‑1 (MQ=255)
                                                               tttttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTg                                                            >  2:2714912/1‑126 (MQ=255)
                                                               tttttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTg                                                            <  2:384242/126‑1 (MQ=255)
                                                                ttttttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGa                                                           <  1:2062232/126‑1 (MQ=255)
                                                                    ttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTa                                                       <  1:2720774/126‑1 (MQ=255)
                                                                    ttCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTa                                                       <  2:1453877/126‑1 (MQ=255)
                                                                      cGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAgg                                                     <  2:1983147/126‑1 (MQ=255)
                                                                      cGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAgg                                                     >  1:2292726/1‑126 (MQ=255)
                                                                      cGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAgg                                                     >  2:3373912/1‑126 (MQ=255)
                                                                       ggCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGc                                                    <  2:2380480/126‑1 (MQ=255)
                                                                         ccATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCaa                                                  <  1:2663060/126‑1 (MQ=255)
                                                                         ccATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCaa                                                  >  1:635534/1‑126 (MQ=255)
                                                                            tGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCa                                               <  1:1012445/126‑1 (MQ=255)
                                                                            tGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCa                                               <  2:217706/126‑1 (MQ=255)
                                                                            tGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCa                                               <  2:729485/126‑1 (MQ=255)
                                                                            tGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCa                                               >  1:907845/1‑126 (MQ=255)
                                                                              gggtGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAgg                                             <  1:2668799/123‑1 (MQ=255)
                                                                               gTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGc                                            <  1:3263786/126‑1 (MQ=255)
                                                                                ttGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGATGTAGGCAAGCAGGCt                                           <  1:3191253/126‑1 (MQ=255)
                                                                                 tGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCtt                                          >  2:593749/1‑126 (MQ=255)
                                                                                  gATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTa                                         <  1:99633/126‑1 (MQ=255)
                                                                                   aTACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTaa                                        >  2:2217697/1‑126 (MQ=255)
                                                                                    tACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAg                                       >  1:2044830/1‑126 (MQ=255)
                                                                                      cctcctTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGa                                     <  1:1844313/126‑1 (MQ=255)
                                                                                      cctcctTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGa                                     <  2:2627743/126‑1 (MQ=255)
                                                                                      cctcctTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGa                                     <  1:475883/126‑1 (MQ=255)
                                                                                        tcctTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAAt                                   >  1:405145/1‑126 (MQ=255)
                                                                                         cctTTGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATc                                  <  2:1058395/126‑1 (MQ=255)
                                                                                            ttGTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATcgcg                               >  2:1791710/1‑126 (MQ=255)
                                                                                              gTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCggg                             <  1:254030/126‑1 (MQ=255)
                                                                                              gTTAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCggg                             >  1:1120678/1‑126 (MQ=255)
                                                                                               ttAACACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGc                            >  2:1214628/1‑126 (MQ=255)
                                                                                                 aaCACCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCa                          <  1:1189480/126‑1 (MQ=255)
                                                                                                  acacCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAg                         <  2:997618/126‑1 (MQ=255)
                                                                                                   cacCCGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATAAGGGATTATTGGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAgg                        >  1:1074637/1‑126 (MQ=255)
                                                                                                    ccccGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAggg                       <  2:2756344/125‑1 (MQ=255)
                                                                                                       cGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCtt                    <  2:2473714/126‑1 (MQ=255)
                                                                                                       cGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCtt                    >  1:979947/1‑126 (MQ=255)
                                                                                                       cGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCtt                    >  1:766855/1‑126 (MQ=255)
                                                                                                       cGTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCtt                    >  1:2795834/1‑126 (MQ=255)
                                                                                                        gTAAAAAAAGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCttt                   >  2:1370648/1‑126 (MQ=255)
                                                                                                           aaaaaaGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCa                >  1:2387261/1‑126 (MQ=255)
                                                                                                           aaaaaaGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCa                <  1:1262361/126‑1 (MQ=255)
                                                                                                            aaaaaGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAt               >  1:1885836/1‑126 (MQ=255)
                                                                                                            aaaaaGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAt               >  1:2095260/1‑126 (MQ=255)
                                                                                                             aaaaGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAtt              >  1:2583392/1‑126 (MQ=255)
                                                                                                              aaaGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAttt             >  1:657105/1‑126 (MQ=255)
                                                                                                               aaGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAtttt            >  1:375151/1‑126 (MQ=255)
                                                                                                               aaGGGCGCAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCAtttt            <  2:1232163/126‑1 (MQ=255)
                                                                                                                    cgcAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGc       >  1:3079462/1‑126 (MQ=255)
                                                                                                                     gcAACATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCg      <  2:649529/126‑1 (MQ=255)
                                                                                                                         cATGATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACgg  >  1:2388125/1‑126 (MQ=255)
                                                                                                                            |                                                                                                                          
ACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAGGCAAGCAGGCTTAAGGAATCGCGGGCCAGGGCTTTGCATTTTTTAGCGACGG  >  CP009273/132876‑133121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑