Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,537,666 G→T L180M (CTG→ATG)  bioH ← pimeloyl‑ACP methyl ester carboxylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,537,6660GT99.5% 715.5 / NA 192L180M (CTG→ATG) bioHpimeloyl‑ACP methyl ester carboxylesterase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base T (86/104);  minor base C (0/1);  total (86/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

GTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTG  >  CP009273/3537543‑3537788
                                                                                                                           |                                                                                                                          
gTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATcc                                                                                                                          <  1:712889/126‑1 (MQ=255)
 tttATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATccc                                                                                                                         <  2:360618/126‑1 (MQ=255)
 tttATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGAATTCCATccc                                                                                                                         >  2:228882/1‑126 (MQ=255)
       cAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCAtt                                                                                                                   <  1:1015653/126‑1 (MQ=255)
       cAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCAtt                                                                                                                   <  2:3109930/126‑1 (MQ=255)
       cAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCAtt                                                                                                                   <  1:1244226/126‑1 (MQ=255)
        aGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTa                                                                                                                  >  2:1628853/1‑126 (MQ=255)
         gCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTaa                                                                                                                 >  1:812396/1‑126 (MQ=255)
          cATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAg                                                                                                                >  2:568785/1‑126 (MQ=255)
          cATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAg                                                                                                                >  2:400432/1‑126 (MQ=255)
           aTCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGc                                                                                                               >  1:2559155/1‑126 (MQ=255)
            tCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCa                                                                                                              <  1:851399/126‑1 (MQ=255)
             cGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCAc                                                                                                             <  2:3201414/126‑1 (MQ=255)
              ggCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACg                                                                                                            >  1:3187451/1‑126 (MQ=255)
               gCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGt                                                                                                           <  1:2698206/126‑1 (MQ=255)
                caccacTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTc                                                                                                          >  2:1397768/1‑126 (MQ=255)
                 accacTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCa                                                                                                         <  1:2360922/126‑1 (MQ=255)
                  ccacTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCaa                                                                                                        >  1:2066675/1‑126 (MQ=255)
                   cacTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAAc                                                                                                       <  2:3009007/126‑1 (MQ=255)
                    acTTTGCGCGGCGCCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAAcc                                                                                                      <  2:2697946/126‑1 (MQ=255)
                    acTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAAcc                                                                                                      >  2:2045591/1‑126 (MQ=255)
                       ttGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTcc                                                                                                   <  2:3147928/126‑1 (MQ=255)
                        tGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCg                                                                                                  <  2:3023839/126‑1 (MQ=255)
                        tGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCg                                                                                                  >  1:479003/1‑126 (MQ=255)
                          cgcgGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGc                                                                                                <  2:1926069/126‑1 (MQ=255)
                             ggCACCAGACCGTCGAGATAGCCATACAATCGCAAAACCGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATc                                                                                             >  1:3408569/1‑126 (MQ=255)
                             ggCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATc                                                                                             >  2:3292423/1‑126 (MQ=255)
                             ggCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATc                                                                                             <  2:1304535/126‑1 (MQ=255)
                                aCCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGt                                                                                          <  1:1573791/126‑1 (MQ=255)
                                 ccAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTa                                                                                         <  2:222769/126‑1 (MQ=255)
                                 ccAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTa                                                                                         >  1:933729/1‑126 (MQ=255)
                                 ccAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTa                                                                                         >  1:501871/1‑126 (MQ=255)
                                  cAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTaa                                                                                        <  1:1796214/126‑1 (MQ=255)
                                  cAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTaa                                                                                        <  2:1242146/126‑1 (MQ=255)
                                   aGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAAc                                                                                       >  2:2397104/1‑126 (MQ=255)
                                   aGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAAc                                                                                       >  2:1596910/1‑126 (MQ=255)
                                   aGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAAc                                                                                       <  2:727140/126‑1 (MQ=255)
                                     aCCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGc                                                                                     >  1:2347813/1‑126 (MQ=255)
                                       cGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAGGCACGTCAACCTCCGGCATCGGTAACGCCa                                                                                   >  1:775049/1‑126 (MQ=255)
                                       cGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCa                                                                                   >  2:2532175/1‑126 (MQ=255)
                                        gTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAg                                                                                  <  2:2492728/126‑1 (MQ=255)
                                           gagaTAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAAc                                                                               <  2:2911931/126‑1 (MQ=255)
                                            agaTAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACg                                                                              <  1:2185746/126‑1 (MQ=255)
                                            agaTAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACg                                                                              <  1:539056/126‑1 (MQ=255)
                                               tAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGtt                                                                           >  2:1742818/1‑126 (MQ=255)
                                                aGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGttt                                                                          >  1:2093716/1‑126 (MQ=255)
                                                  ccATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTttc                                                                        >  2:1654149/1‑126 (MQ=255)
                                                  ccATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTttc                                                                        <  1:600131/126‑1 (MQ=255)
                                                     tACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTttcttc                                                                     >  2:3248840/1‑126 (MQ=255)
                                                      aCAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCa                                                                    >  1:2016800/1‑126 (MQ=255)
                                                        aaTCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAAc                                                                  >  2:1169182/1‑126 (MQ=255)
                                                          tCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGc                                                                <  2:2587135/126‑1 (MQ=255)
                                                          tCGCAAAAACGGCATGGACACGTTTTGCAGCCGCTGACGGAGATCGACCGTTTTAAGGATTTCCATCCCGCCATTAAGCACTTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGc                                                                <  1:2429035/126‑1 (MQ=255)
                                                           cGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGcc                                                               <  2:933729/126‑1 (MQ=255)
                                                             cAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCcg                                                             <  2:3126207/126‑1 (MQ=255)
                                                             cAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCcg                                                             <  2:3181373/126‑1 (MQ=255)
                                                                 aaCGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCa                                                         >  2:1505801/1‑126 (MQ=255)
                                                                      cATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTg                                                    <  1:1608252/126‑1 (MQ=255)
                                                                       aTGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTgg                                                   <  1:1175870/126‑1 (MQ=255)
                                                                        ttgACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCATGATTTCCATCTCGCCATTAAGCACGTCTACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGgc                                                  <  1:2865820/124‑1 (MQ=255)
                                                                        tGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGgc                                                  >  1:3335855/1‑126 (MQ=255)
                                                                         ggACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGgcg                                                 >  2:2190129/1‑126 (MQ=255)
                                                                           acacGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGgcgcg                                               <  1:2653345/126‑1 (MQ=255)
                                                                            cacGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGgcgcgc                                              >  1:1530993/1‑126 (MQ=255)
                                                                            cacGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGgcgcgc                                              >  1:1015024/1‑126 (MQ=255)
                                                                             acGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGcc                                             >  2:798391/1‑126 (MQ=255)
                                                                              cGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCg                                            >  1:1771386/1‑126 (MQ=255)
                                                                              cGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCg                                            >  1:2945025/1‑126 (MQ=255)
                                                                              cGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCg                                            <  1:318011/126‑1 (MQ=255)
                                                                               gTTTTGCAGAGGCTGACGGATATCGACCGTTTTCAGGTTTTCCATCCCGCCATTAAGCACGTCAACCTCAGGCATCGGTAACGCCAGAACGCTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGt                                           >  1:3426438/1‑126 (MQ=255)
                                                                                 tttGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGttt                                         <  2:699720/126‑1 (MQ=255)
                                                                                  ttGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTc                                        <  1:3222372/126‑1 (MQ=255)
                                                                                     cAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGt                                     >  1:175141/1‑126 (MQ=255)
                                                                                       gCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTcc                                   <  1:1606332/126‑1 (MQ=255)
                                                                                       gCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTcc                                   <  2:2481710/126‑1 (MQ=255)
                                                                                       gCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTcc                                   >  1:148638/1‑126 (MQ=255)
                                                                                          gCTGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCa                                <  1:1505801/126‑1 (MQ=255)
                                                                                            tGACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATg                              >  1:3133017/1‑126 (MQ=255)
                                                                                             gACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATgg                             <  2:2318672/126‑1 (MQ=255)
                                                                                             gACGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATgg                             <  1:2673709/126‑1 (MQ=255)
                                                                                              aCGGAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGt                            <  1:1299004/126‑1 (MQ=255)
                                                                                                ggAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGttt                          <  1:1044026/126‑1 (MQ=255)
                                                                                                ggAGATCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGttt                          >  1:1944799/1‑126 (MQ=255)
                                                                                                 gagaTCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTg                         <  2:2381225/126‑1 (MQ=255)
                                                                                                   gaTCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTa                       <  1:1563393/126‑1 (MQ=255)
                                                                                                   gaTCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTa                       <  2:206471/126‑1 (MQ=255)
                                                                                                    aTCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTaa                      <  2:1271412/126‑1 (MQ=255)
                                                                                                     tCGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAAc                     <  2:1329659/126‑1 (MQ=255)
                                                                                                      cGACCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACg                    <  1:2880779/126‑1 (MQ=255)
                                                                                                        aCCGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGcc                  >  1:576601/1‑126 (MQ=255)
                                                                                                          cGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAg                >  2:2107944/1‑126 (MQ=255)
                                                                                                          cGTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAg                >  1:252586/1‑126 (MQ=255)
                                                                                                           gTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAgg               >  2:1541337/1‑126 (MQ=255)
                                                                                                           gTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAgg               >  1:1844921/1‑126 (MQ=255)
                                                                                                           gTTTTCAGGATTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAgg               >  1:1164398/1‑126 (MQ=255)
                                                                                                                    aTTTCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGTACGCTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTcc      >  2:959687/1‑126 (MQ=255)
                                                                                                                     tttCCATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCa     <  1:1626366/126‑1 (MQ=255)
                                                                                                                        ccATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTg  >  2:623087/1‑126 (MQ=255)
                                                                                                                        ccATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTg  >  1:2328354/1‑126 (MQ=255)
                                                                                                                        ccATCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAATCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTg  <  2:2512815/126‑1 (MQ=255)
                                                                                                                           |                                                                                                                          
GTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTG  >  CP009273/3537543‑3537788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑