Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,436,6360AC20.8% 319.2 / 19.4 106V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (72/12);  new base C (0/22);  total (72/34)
Fisher's exact test for biased strand distribution p-value = 1.78e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 140 total aligned reads displayed.

CGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTG  >  CP009273/3436536‑3436736
                                                                                                    |                                                                                                    
cGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTa                                                                                                      <  1:1236973/101‑1 (MQ=255)
cGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTa                                                                                                      <  1:2296492/101‑1 (MQ=255)
 gAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAc                                                                                                     >  1:674867/1‑101 (MQ=255)
 gAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAc                                                                                                     >  2:2175175/1‑101 (MQ=255)
 gAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAc                                                                                                     >  2:3249364/1‑101 (MQ=255)
  aaaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcc                                                                                                    >  2:1679300/1‑101 (MQ=255)
  aaaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcc                                                                                                    <  1:1354785/101‑1 (MQ=255)
  aaaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcc                                                                                                    <  1:175968/101‑1 (MQ=255)
    aTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcccc                                                                                                  <  2:1454623/101‑1 (MQ=255)
    aTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcccc                                                                                                  >  1:3377088/1‑101 (MQ=255)
    aTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcccc                                                                                                  >  1:3130800/1‑101 (MQ=255)
     ttgttgTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccccc                                                                                                 <  1:1052479/101‑1 (MQ=255)
     ttgttgTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccccc                                                                                                 <  1:1878769/101‑1 (MQ=255)
      tgttttCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCTCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCg                                                                                                <  2:3148063/101‑1 (MQ=255)
      tgttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCg                                                                                                >  1:3408852/1‑101 (MQ=255)
      tgttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCg                                                                                                >  2:2580609/1‑101 (MQ=255)
      tgttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCg                                                                                                >  2:2845951/1‑101 (MQ=255)
           tCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCata                                                                                           >  1:2743608/1‑101 (MQ=255)
                cAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCAc                                                                                      >  2:582268/1‑101 (MQ=255)
                 aGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACt                                                                                     <  1:2219149/101‑1 (MQ=255)
                  gtcccACCCAGTCCCCCCCCCGAACCATGCCGCGGTGGCCGCCGGGAACAGAAAAATACTGGAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACtt                                                                                    <  2:2865105/98‑1 (MQ=255)
                  gTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACtt                                                                                    >  1:2436264/1‑101 (MQ=255)
                   ttCCAACCAGTCCCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACttt                                                                                   <  1:1646512/101‑1 (MQ=255)
                   ttCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACttt                                                                                   >  2:2953622/1‑101 (MQ=255)
                     ccAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCa                                                                                 >  2:477828/1‑101 (MQ=255)
                       aaCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGc                                                                               >  1:2225164/1‑101 (MQ=255)
                         ccAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCgg                                                                             >  1:3490188/1‑101 (MQ=255)
                         ccAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCgg                                                                             >  2:3254882/1‑101 (MQ=255)
                          cAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGt                                                                            >  1:1506247/1‑101 (MQ=255)
                                cGCCCCGGAACCAGGACGCGATGGTCGCGGGAAACAAAAAAATACTGGAGGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgt                                                                      <  2:1000397/101‑1 (MQ=255)
                                 ccccccAACCCAGGAGGGGAGGGCCGCGGGGAAAAAAAAAATACGGGAAGCGAAGATGGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                     <  2:702611/100‑1 (MQ=255)
                                  cccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                    >  1:2534955/1‑101 (MQ=255)
                                   ccccGACCCAGGACGGGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                   <  2:3925019/101‑1 (MQ=255)
                                   ccccGACCCAGGACGGGATGGCCGCGGGAAACAAAATAATACGGGAAGGGAAGATTGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                   <  2:2056516/101‑1 (MQ=255)
                                   ccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                   <  1:3169823/101‑1 (MQ=255)
                                   ccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                   >  1:3300271/1‑101 (MQ=255)
                                         aCCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgt                                                             <  1:2084303/101‑1 (MQ=255)
                                          ccATGACGCGATGGCCGCCGGAAAAAAAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg                                                            <  2:3091119/101‑1 (MQ=255)
                                           cATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                           >  1:2794880/1‑101 (MQ=255)
                                            aTGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgca                                                          >  1:2708335/1‑101 (MQ=255)
                                               aCGCGATGGTCGCCGGGAAAAGAATAATACTGGAAGCGAAGATTGCCGGAATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagca                                                       <  1:487436/101‑1 (MQ=255)
                                                cgcgATGGTCGCGGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                      <  1:1986759/101‑1 (MQ=255)
                                                cgcgATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                      >  2:2589245/1‑101 (MQ=255)
                                                 gcgATGGTCGCGGGGAACAGAATAATACTGGAAGCGAAGATTGCGGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATa                                                     <  1:2821087/101‑1 (MQ=255)
                                                  cgttGGTCGCCGGGAACAGAATATTTCTGGAGGAAAGGTGTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAg                                                    <  2:2751143/98‑1 (MQ=255)
                                                    agggTCGCCGGGAAAAAAATAATACTGGAAGCAAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                  <  1:3743769/99‑1 (MQ=255)
                                                     tGGTCCCGGGGAAAACAAAAATAATGGAAGCGATGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                 <  2:3654269/101‑1 (MQ=255)
                                                      cgccgcgGGGAAAAAAAAAATACTGGAAGCGAAGAGTGCCGTCATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCATCATAGacac                                                <  2:1738180/98‑1 (MQ=255)
                                                       gTCGCGGGGAACAAAATAATCCTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                               <  2:3489483/101‑1 (MQ=255)
                                                       gTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                               >  1:512246/1‑101 (MQ=255)
                                                       gTCGCCGGAAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                               <  2:3119512/101‑1 (MQ=255)
                                                        tCGACGGCAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGCGCTCTGTGCAGCATAGACacga                                              <  1:3345926/101‑1 (MQ=255)
                                                         cGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                             >  2:2933657/1‑101 (MQ=255)
                                                         cGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                             >  1:132645/1‑101 (MQ=255)
                                                           ccGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACAcgacga                                           >  2:3544387/1‑101 (MQ=255)
                                                            ggggAAAAAAATAATACGGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACgacgac                                          <  2:1200572/100‑1 (MQ=255)
                                                            cGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACgacgac                                          >  2:2036585/1‑101 (MQ=255)
                                                            cGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACgacgac                                          >  1:3590075/1‑101 (MQ=255)
                                                            cGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACgacgac                                          >  2:1366004/1‑101 (MQ=255)
                                                             gggAACAAAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGAcc                                         <  2:1850472/101‑1 (MQ=255)
                                                              ggAACAGAATAATACTGGAAGCGAAGATTGCGGGTATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                        <  1:1072100/101‑1 (MQ=255)
                                                                aaCTGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTg                                      >  2:1349578/1‑101 (MQ=255)
                                                                aaCAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTg                                      >  1:846134/1‑101 (MQ=255)
                                                                 aCAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGc                                     >  1:1124488/1‑101 (MQ=255)
                                                                 aCAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGc                                     >  1:3422300/1‑101 (MQ=255)
                                                                  cAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCt                                    <  1:1374859/101‑1 (MQ=255)
                                                                   aaaataatACTGGAAGCGAAGATGGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                   <  2:2654888/99‑1 (MQ=255)
                                                                    gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGa                                  >  2:829075/1‑101 (MQ=255)
                                                                    aaataatACGGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGa                                  <  2:733971/100‑1 (MQ=255)
                                                                     aataatACTGAAAGCGAAGTTGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                 <  1:365826/101‑1 (MQ=255)
                                                                     aataatACGGGAAGCGAAGTTTGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                 <  2:920651/101‑1 (MQ=255)
                                                                      ataataCTGGAAGGGAAAATGGCGGGCATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                <  2:3872904/101‑1 (MQ=255)
                                                                       taataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                               >  2:1571545/1‑101 (MQ=255)
                                                                        aataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                              >  1:201200/1‑101 (MQ=255)
                                                                        aataCGGGAGGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                              <  2:599318/101‑1 (MQ=255)
                                                                         ataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                             >  1:2239422/1‑101 (MQ=255)
                                                                          taCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                            >  1:3516944/1‑101 (MQ=255)
                                                                           aCTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcg                           <  1:1170653/101‑1 (MQ=255)
                                                                              ggAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                        >  1:722914/1‑101 (MQ=255)
                                                                                aaGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAg                      >  2:815097/1‑101 (MQ=255)
                                                                                aaGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAg                      >  2:1161691/1‑101 (MQ=255)
                                                                                   cGAAGATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                   <  2:2351620/101‑1 (MQ=255)
                                                                                   cGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                   <  1:2617026/101‑1 (MQ=255)
                                                                                   cGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                   >  1:3189270/1‑101 (MQ=255)
                                                                                   cGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                   >  2:382225/1‑101 (MQ=255)
                                                                                    gAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTa                  >  1:1569118/1‑101 (MQ=255)
                                                                                      aGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAcc                >  1:1691501/1‑101 (MQ=255)
                                                                                       gATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACca               >  1:3746973/1‑101 (MQ=255)
                                                                                       gATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACca               >  1:2903365/1‑101 (MQ=255)
                                                                                        aTTGCCGTGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac              <  1:570454/101‑1 (MQ=255)
                                                                                        aTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac              <  1:1679300/101‑1 (MQ=255)
                                                                                         ttGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca             >  2:2015639/1‑101 (MQ=255)
                                                                                            ccGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATg          <  1:430728/101‑1 (MQ=255)
                                                                                            ccGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATg          >  2:2176291/1‑101 (MQ=255)
                                                                                              gggATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg        >  1:3887182/1‑101 (MQ=255)
                                                                                               ggATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcgg       >  1:2092220/1‑101 (MQ=255)
                                                                                                gATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc      <  1:1699426/101‑1 (MQ=255)
                                                                                                gATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc      >  2:3944708/1‑101 (MQ=255)
                                                                                                 aTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcg     <  1:2529839/101‑1 (MQ=255)
                                                                                                    aCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg  >  2:2854838/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTG  >  CP009273/3436536‑3436736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑