Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,618,035 G→A A302T (GCC→ACC)  nadR → trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,618,0350GA100.0% 652.3 / NA 160A302T (GCC→ACC) nadRtrifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (84/76);  total (84/76)

Only 100 of 160 total aligned reads displayed.

TTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGA  >  CP009273/4617937‑4618131
                                                                                                  |                                                                                                
   cacaCCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAAt                                                                                             >  2:2308548/1‑101 (MQ=255)
      acCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATaaa                                                                                          >  2:1691768/1‑101 (MQ=255)
      acCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATaaa                                                                                          <  2:65156/101‑1 (MQ=255)
        cTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGt                                                                                        <  2:880283/101‑1 (MQ=255)
           ggcggTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGc                                                                                     <  1:2407103/101‑1 (MQ=255)
           ggcggTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGc                                                                                     >  1:3840184/1‑101 (MQ=255)
              ggTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCAtt                                                                                  >  2:3378535/1‑101 (MQ=255)
              ggTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCAtt                                                                                  >  2:2057156/1‑101 (MQ=255)
              ggTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCAtt                                                                                  >  2:93199/1‑101 (MQ=255)
              ggTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCAtt                                                                                  <  2:1298054/101‑1 (MQ=255)
               gTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCAttt                                                                                 >  2:3141180/1‑101 (MQ=255)
               gTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCAttt                                                                                 <  2:1273775/101‑1 (MQ=255)
               gTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCAttt                                                                                 <  1:3263399/101‑1 (MQ=255)
                tgatgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTa                                                                                <  1:1096418/101‑1 (MQ=255)
                 gatgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTAt                                                                               >  2:3049567/1‑101 (MQ=255)
                 gatgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTAt                                                                               >  2:476570/1‑101 (MQ=255)
                 gatgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTAt                                                                               >  2:2219626/1‑101 (MQ=255)
                 gatgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTAt                                                                               >  1:830156/1‑101 (MQ=255)
                 gatgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTAt                                                                               <  2:944837/101‑1 (MQ=255)
                 gatgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTAt                                                                               <  1:189836/101‑1 (MQ=255)
                  atgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATc                                                                              <  1:2398420/101‑1 (MQ=255)
                  atgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATc                                                                              <  1:552878/101‑1 (MQ=255)
                   tgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCg                                                                             <  2:1105701/101‑1 (MQ=255)
                    gagaTCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGa                                                                            >  1:743130/1‑101 (MQ=255)
                     agaTCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGAt                                                                           <  2:1502105/101‑1 (MQ=255)
                      gaTCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATa                                                                          >  1:2068029/1‑101 (MQ=255)
                         cGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCg                                                                       <  1:3436042/101‑1 (MQ=255)
                           cATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGAt                                                                     >  2:1791851/1‑101 (MQ=255)
                           cATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGAt                                                                     >  1:139499/1‑101 (MQ=255)
                             ttGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGAttt                                                                   <  2:2201271/101‑1 (MQ=255)
                              tGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGAtttt                                                                  <  2:916044/101‑1 (MQ=255)
                               gCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTg                                                                 >  2:1990312/1‑101 (MQ=255)
                               gCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTg                                                                 <  1:2674832/101‑1 (MQ=255)
                                  gTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTca                                                              >  1:2630870/1‑101 (MQ=255)
                                  gTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTca                                                              <  1:3875667/101‑1 (MQ=255)
                                    aTTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTcacc                                                            <  1:3355955/101‑1 (MQ=255)
                                    aTTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTcacc                                                            >  1:2009719/1‑101 (MQ=255)
                                     ttCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATATCGATTTTGTcacca                                                           >  1:1686804/1‑101 (MQ=255)
                                        tGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTc                                                        >  1:1094729/1‑101 (MQ=255)
                                         gACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCa                                                       <  1:1885571/101‑1 (MQ=255)
                                         gACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCa                                                       >  2:1627713/1‑101 (MQ=255)
                                          aCTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAg                                                      <  1:163146/101‑1 (MQ=255)
                                           cTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAgg                                                     <  1:2776363/101‑1 (MQ=255)
                                            tACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGc                                                    >  1:3182333/1‑101 (MQ=255)
                                            tACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGc                                                    <  1:1868912/101‑1 (MQ=255)
                                                aTAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTc                                                <  1:2040395/101‑1 (MQ=255)
                                                 tAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCt                                               <  1:3802748/101‑1 (MQ=255)
                                                    aaTCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCa                                            >  1:2907443/1‑101 (MQ=255)
                                                    aaTCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCa                                            >  2:2182491/1‑101 (MQ=255)
                                                      tCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCaaa                                          <  1:3627637/101‑1 (MQ=255)
                                                         cgcTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAg                                       >  2:260242/1‑101 (MQ=255)
                                                         cgcTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAg                                       >  2:338518/1‑101 (MQ=255)
                                                          gcTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGt                                      >  2:842662/1‑101 (MQ=255)
                                                           cTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTa                                     <  2:1056383/101‑1 (MQ=255)
                                                           cTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTa                                     >  2:3631557/1‑101 (MQ=255)
                                                            tGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTAc                                    >  2:3405735/1‑101 (MQ=255)
                                                            tGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTAc                                    >  1:596724/1‑101 (MQ=255)
                                                            tGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTAc                                    <  2:314257/101‑1 (MQ=255)
                                                              ggCCACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGa                                  <  1:222624/101‑1 (MQ=255)
                                                                 cACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAgg                               >  1:951958/1‑101 (MQ=255)
                                                                 cACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAgg                               >  1:961702/1‑101 (MQ=255)
                                                                 cACGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAgg                               >  2:25288/1‑101 (MQ=255)
                                                                   cGCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGc                             <  1:1271266/101‑1 (MQ=255)
                                                                    gCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCg                            <  1:2885853/101‑1 (MQ=255)
                                                                    gCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCg                            >  2:2431299/1‑101 (MQ=255)
                                                                    gCTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCg                            >  2:3075722/1‑101 (MQ=255)
                                                                     cTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCgg                           >  1:628855/1‑101 (MQ=255)
                                                                     cTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCgg                           >  1:1736916/1‑101 (MQ=255)
                                                                     cTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCgg                           <  1:2314836/101‑1 (MQ=255)
                                                                     cTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCgg                           >  2:2723301/1‑101 (MQ=255)
                                                                     cTCAATACATTGATTTTGCGGTGAAATATACCAATAAAGAGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCgg                           >  2:3461559/1‑101 (MQ=255)
                                                                      tCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCggg                          >  1:3546374/1‑101 (MQ=255)
                                                                      tCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCggg                          >  1:2731866/1‑101 (MQ=255)
                                                                      tCAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCggg                          >  2:2774585/1‑101 (MQ=255)
                                                                       cAATACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGa                         >  2:802418/1‑101 (MQ=255)
                                                                         aTACATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAAc                       <  1:1576757/101‑1 (MQ=255)
                                                                           aCATTTATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACAt                     <  2:1452260/101‑1 (MQ=255)
                                                                           aCATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACAt                     <  1:1691768/101‑1 (MQ=255)
                                                                            cATTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATc                    >  2:159445/1‑101 (MQ=255)
                                                                             aTTGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATcc                   <  2:3933636/101‑1 (MQ=255)
                                                                               tGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGt                 <  1:20891/101‑1 (MQ=255)
                                                                               tGATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGt                 >  1:192527/1‑101 (MQ=255)
                                                                                gATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGtt                >  2:3868712/1‑101 (MQ=255)
                                                                                gATTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGtt                >  2:1894507/1‑101 (MQ=255)
                                                                                 aTTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTc               >  2:1166366/1‑101 (MQ=255)
                                                                                 aTTTTGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTc               <  2:69794/101‑1 (MQ=255)
                                                                                   tttGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGt             <  1:2932660/101‑1 (MQ=255)
                                                                                   tttGCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGt             <  2:503211/101‑1 (MQ=255)
                                                                                      gCGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCa          >  1:3600397/1‑101 (MQ=255)
                                                                                       cGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAg         >  2:3480854/1‑101 (MQ=255)
                                                                                       cGGTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAg         <  1:1316643/101‑1 (MQ=255)
                                                                                         gTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGgc       <  2:1910093/101‑1 (MQ=255)
                                                                                         gTGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGgc       <  2:2024140/101‑1 (MQ=255)
                                                                                          tGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGgcg      >  1:3576211/1‑101 (MQ=255)
                                                                                          tGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGgcg      <  2:43710/101‑1 (MQ=255)
                                                                                          tGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGgcg      <  2:3780900/101‑1 (MQ=255)
                                                                                          tGAAATATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGgcg      >  1:2658257/1‑101 (MQ=255)
                                                                                            aaaTATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCt    <  2:2550378/101‑1 (MQ=255)
                                                                                             aaTATACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGgcgcgg   >  2:373551/1‑99 (MQ=255)
                                                                                              atatACCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCtga  >  1:3898488/1‑101 (MQ=255)
                                                                                                  |                                                                                                
TTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGA  >  CP009273/4617937‑4618131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑