Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,325,806 C→T R168C (CGT→TGT)  topA → DNA topoisomerase I, omega subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,325,8060CT99.2% 490.3 / ‑4.6 124R168C (CGT→TGT) topADNA topoisomerase I, omega subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (69/54);  total (69/55)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 124 total aligned reads displayed.

CCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGG  >  CP009273/1325709‑1325902
                                                                                                 |                                                                                                
ccGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTc                                                                                               >  1:553739/1‑101 (MQ=255)
 cGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTcn                                                                                              <  1:1353591/101‑2 (MQ=255)
  gAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGATGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGc                                                                                             <  2:3883957/101‑1 (MQ=255)
   aGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCt                                                                                            <  2:2110526/101‑1 (MQ=255)
     tggtgTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCttt                                                                                          >  1:3254086/1‑101 (MQ=255)
      ggtgTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTa                                                                                         <  1:549509/101‑1 (MQ=255)
       gtgtTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTAt                                                                                        >  1:3367022/1‑101 (MQ=255)
         gtTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATgg                                                                                      >  2:789076/1‑101 (MQ=255)
         gtTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATgg                                                                                      >  1:434989/1‑101 (MQ=255)
         gtTTAACGAAATTACTAAAAACGCGATCCGCCACGCATTTAACAAACCGGGCGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATgg                                                                                      >  1:3307311/1‑101 (MQ=255)
           ttAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGAc                                                                                    <  2:1155924/101‑1 (MQ=255)
            tAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGAcc                                                                                   >  2:149660/1‑101 (MQ=255)
            tAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGAcc                                                                                   >  1:2883139/1‑101 (MQ=255)
            tAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGAcc                                                                                   <  1:1233483/101‑1 (MQ=255)
             aaCGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACcg                                                                                  <  1:3551151/101‑1 (MQ=255)
             aaCGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACcg                                                                                  <  2:3287707/101‑1 (MQ=255)
              aCGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACcgc                                                                                 >  1:1608478/1‑101 (MQ=255)
              aCGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACcgc                                                                                 >  2:1288940/1‑101 (MQ=255)
               cGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACcgcg                                                                                <  2:3859283/101‑1 (MQ=255)
               cGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACcgcg                                                                                >  2:2788011/1‑101 (MQ=255)
                gAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCgt                                                                               >  2:3454039/1‑101 (MQ=255)
                gAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCgt                                                                               <  1:3265996/101‑1 (MQ=255)
                gAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCgt                                                                               >  2:3836840/1‑101 (MQ=255)
                gAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCgt                                                                               <  2:3315696/101‑1 (MQ=255)
                 aaaTTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCgtg                                                                              >  1:1095399/1‑101 (MQ=255)
                     tACTAAAAACGCGATCCGCCGGGCATTTAACAAACCGGGTGAGCTGAATATTGCTCGTTTTAAGCCCCAGC‑GGCGTGTCGCTTTATGGACCGCGTGGTggg                                                                         <  2:369171/101‑1 (MQ=255)
                     tACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGtggtgg                                                                          <  1:528225/101‑1 (MQ=255)
                         aaaaaCGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGta                                                                      >  1:780603/1‑101 (MQ=255)
                          aaaaCGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGtat                                                                     <  1:3525123/101‑1 (MQ=255)
                          aaaaCGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCCAGGTGGGGtat                                                                     >  1:1372707/1‑101 (MQ=255)
                            agcgcgATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTatat                                                                   <  2:806896/99‑1 (MQ=255)
                             aCGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATg                                                                  <  2:385752/101‑1 (MQ=255)
                             aCGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATg                                                                  >  2:2956468/1‑101 (MQ=255)
                               gcgATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGt                                                                <  1:1141429/101‑1 (MQ=255)
                                cgATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGtt                                                               >  1:2947453/1‑101 (MQ=255)
                                cgATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGtt                                                               <  1:2419449/101‑1 (MQ=255)
                                 gATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGttt                                                              <  2:959101/101‑1 (MQ=255)
                                 gATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGttt                                                              <  2:190796/101‑1 (MQ=255)
                                 gATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGttt                                                              <  2:54974/101‑1 (MQ=255)
                                 gATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGCATATGGttt                                                              >  2:1457551/1‑101 (MQ=255)
                                  aTCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTc                                                             <  2:1633855/101‑1 (MQ=255)
                                  aTCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTc                                                             <  2:2947453/101‑1 (MQ=255)
                                  aTCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTc                                                             <  1:152301/101‑1 (MQ=255)
                                  aTCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTc                                                             >  2:451512/1‑101 (MQ=255)
                                  aTCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGGATATGGTTTc                                                             >  1:1363624/1‑101 (MQ=255)
                                   tCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTcg                                                            >  1:3771519/1‑101 (MQ=255)
                                     cGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTcgcc                                                          >  2:1379667/1‑101 (MQ=255)
                                     cGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTcgcc                                                          <  2:1053025/101‑1 (MQ=255)
                                      gCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTcgccg                                                         <  1:1012166/101‑1 (MQ=255)
                                      gCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTcgccg                                                         <  2:2993183/101‑1 (MQ=255)
                                      gCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGGATATGGTTTcgccg                                                         >  1:22918/1‑101 (MQ=255)
                                       ccAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTcgccgc                                                        >  2:270500/1‑101 (MQ=255)
                                         aGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCgctg                                                      <  1:789076/101‑1 (MQ=255)
                                         aGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCgctg                                                      <  2:1303889/101‑1 (MQ=255)
                                         aGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCgctg                                                      >  2:3435210/1‑101 (MQ=255)
                                            cATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTa                                                   >  1:1662191/1‑101 (MQ=255)
                                               ttAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATgg                                                <  1:1080415/101‑1 (MQ=255)
                                                 aaCAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGaa                                              >  1:1221411/1‑101 (MQ=255)
                                                 aaCAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGaa                                              <  1:3776199/101‑1 (MQ=255)
                                                  aCAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGaaa                                             <  1:915128/101‑1 (MQ=255)
                                                   cAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGaaaa                                            >  2:590793/1‑101 (MQ=255)
                                                   cAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGaaaa                                            >  2:1715308/1‑101 (MQ=255)
                                                   cAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGaaaa                                            <  2:3237905/101‑1 (MQ=255)
                                                    aaaCCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGaaaaa                                           >  1:883916/1‑101 (MQ=255)
                                                    aaaCCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGaaaaa                                           >  2:1282545/1‑101 (MQ=255)
                                                    aaaCCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGaaaaa                                           >  2:1846198/1‑101 (MQ=255)
                                                        cGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATc                                       <  2:2244157/101‑1 (MQ=255)
                                                        cGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATc                                       <  2:2392642/101‑1 (MQ=255)
                                                        cGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGCTTCGCCGCTGCTATGGAAAAAGATc                                       >  1:592923/1‑101 (MQ=255)
                                                         gggTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCg                                      >  1:2024458/1‑101 (MQ=255)
                                                         gggTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCg                                      <  2:1702787/101‑1 (MQ=255)
                                                          ggTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGc                                     >  1:1544245/1‑101 (MQ=255)
                                                          ggTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGc                                     <  2:497919/101‑1 (MQ=255)
                                                          ggTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGc                                     >  2:3343711/1‑101 (MQ=255)
                                                           gTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCt                                    <  1:3540490/101‑1 (MQ=255)
                                                            tGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTc                                   <  2:2343/101‑1 (MQ=255)
                                                             gAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCg                                  >  2:2357770/1‑101 (MQ=255)
                                                              aGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGt                                 >  1:374102/1‑101 (MQ=255)
                                                              aGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGt                                 >  1:2941822/1‑101 (MQ=255)
                                                               gCTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTg                                <  1:2014365/101‑1 (MQ=255)
                                                                cTGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTgg                               >  2:2525076/1‑101 (MQ=255)
                                                                 tGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGc                              >  1:2822153/1‑101 (MQ=255)
                                                                 tGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGc                              >  2:2739262/1‑101 (MQ=255)
                                                                 tGAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGc                              >  2:3563665/1‑101 (MQ=255)
                                                                  gAATATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGcc                             >  2:3063609/1‑101 (MQ=255)
                                                                   aaTATTGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCt                            >  1:2648498/1‑101 (MQ=255)
                                                                        tGATCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTg                       >  1:1229972/1‑101 (MQ=255)
                                                                          aTCGTGTTAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGcc                     <  1:1119542/101‑1 (MQ=255)
                                                                                 tAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCgtg              <  1:1093090/101‑1 (MQ=255)
                                                                                 tAATGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCgtg              >  2:236460/1‑101 (MQ=255)
                                                                                   aTGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGtgtg            >  2:3557110/1‑101 (MQ=255)
                                                                                    tGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGc           >  1:2166782/1‑101 (MQ=255)
                                                                                    tGCCCAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGc           >  1:2478734/1‑101 (MQ=255)
                                                                                       ccAGCAGGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGt        >  1:1604161/1‑101 (MQ=255)
                                                                                        cagcagGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTc       >  1:2284191/1‑101 (MQ=255)
                                                                                        cagcagGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTc       >  2:2497789/1‑101 (MQ=255)
                                                                                         agcagGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCg      <  1:1696586/101‑1 (MQ=255)
                                                                                            agGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTg   <  2:1579898/101‑1 (MQ=255)
                                                                                            agGCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTg   >  1:3527675/1‑101 (MQ=255)
                                                                                             ggCGTGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTgg  >  2:136348/1‑101 (MQ=255)
                                                                                                 |                                                                                                
CCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGG  >  CP009273/1325709‑1325902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑