New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	4204319 =	47 (1.370)	4 (0.130)	4/302	2.4	9.5%	coding (112/930 nt)	metA	homoserine O‑transsuccinylase
	?	CP009273	4204505 =	32 (1.000)					coding (298/930 nt)	metA	homoserine O‑transsuccinylase
Rejected: Frequency below/above cutoff threshold.

TTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGC‑AAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCAC  >  CP009273/4204268‑4204775                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                tttGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTACAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTCCACCGCTGGGccc                                                                                                                                                                                                                                            >  2:51906/1‑274 (MQ=255)     tgatgaCAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTgcg                                                                                                                                                                                                                                                       <  1:51907/260‑1 (MQ=255)     ggttgactccgtatcGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGTTCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCtggtg                                                                                                                                                                                                                                        <  2:93568/262‑1 (MQ=255)                 atcGGGCGTCTGGGNAGGAAATTCGTACCCTTTAGGTTCTTATCCTTAACCTGATTCCGAAGAAGATTGAGACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAatgat                                                                                                                                                                                                                            <  2:509815/274‑1 (MQ=255)                                       tCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACt                                                                                                                                                                                                                                                             >  2:124358/1‑220 (MQ=255)                                          tgcACTTAAGTTTCTGTTCCTTAACCTGATGCCGAAGAATATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAAc                                                                                                                                                                                                   <  1:189292/273‑1 (MQ=255)                                          tCCTCTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAAc                                                                                                                                                                                                   <  1:95933/275‑1 (MQ=255)                                          tCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGNTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAAc                                                                                                                                                                                                   <  1:522738/275‑1 (MQ=255)                                           ccACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTg                                                                                                                                                                                                                                                         <  1:124359/220‑1 (MQ=255)                                                                                                                          cACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGa                                                                                                                                                                                                                                                                             >  2:253473/1‑121 (MQ=255)                                                                                                                              tttGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGt                                                                                                                                                                                                                                                                         <  1:253477/121‑1 (MQ=255)                                                                                                                                                           tcctgcataggtgagtttgaaagcagtcgcagaaactgatcttcagtttctatcttcttcggcatcatgttaaggttcataacTTTGACGGTTTGATTGTAACTGGTTCGCCGCTGGGCCTGGTGGAGTTTACTGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGTAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGC‑AAACTCGCACCGaaaa                                                                                  <  1:47515‑M2/191‑1 (MQ=255)                                                                                                                                                           acctgcaaaggtgagtttgaaagcaggcgcagaaactgattttcagtttcaatcttcttcggcatcaggttaaggatcagaacTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAACCAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGAAGCTGTTTGTCTGATGGGCGGGAACGGACGCGCGAAATATCCTCTACGGAAGTCCTAAGCAAAACCCGC‑CCGaaaaa                                                                                 >  2:47514‑M2/84‑275 (MQ=255)                                                                                                                                                                 cccGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGa                                                                                                                                                                                                                                                                             >  2:487232/1‑82 (MQ=255)                                                                                                                                                                     tGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTCCAGATATTCAGGAACAGAACTTTGACGGt                                                                                                                                                                                                                                                                         <  1:487239/82‑1 (MQ=255)                                                                                                                                                                         tCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTc                                                                                                                                                                                   >  2:43623/1‑164 (MQ=255)                                                                                                                                                                          cgctcatctctcccGAAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATNGTAACTGGNGCGCCGCTGGGCATGGTTGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTACGC‑AAACTCGCACCGAAAAACTCTCTGGCGTTTAc                                                                  <  1:468744/264‑1 (MQ=255)                                                                                                                                                                            cgcAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTgcgc                                                                                                                                                                                                                                                      >  1:217108/1‑94 (MQ=255)                                                                                                                                                                             gcAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGaaa                                                                                                                                                                               <  1:43624/164‑1 (MQ=255)                                                                                                                                                                                acacGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCt                                                                                                                                                                                                                                                  <  2:217106/94‑1 (MQ=255)                                                                                                                                                                                      ccGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACgg                                                                                                                                                                                                                                                                          >  1:292880/1‑64 (MQ=255)                                                                                                                                                                                          agagCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTg                                                                                                                                                                                                                                                                      <  2:292876/64‑1 (MQ=255)                                                                                                                                                                                                        acTTCTGCTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGGTTGATTGTAACTGGTGCGCCGCTGGGCATGGTGTCGTTTAATGTTGTCGCTTACTGGCCGCAGATc                                                                                                                                                                                                       >  1:213978/1‑113 (MQ=255)                                                                                                                                                                                                        acTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTg                                                                                                                                                                                                                 >  1:363034/1‑103 (MQ=255)                                                                                                                                                                                                        acTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCCGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAgg                                                                                                                                    >  1:216969/1‑180 (MQ=255)                                                                                                                                                                                                         tttcaatcttcttcggcatcaggttaaggatcagaacTTTGACGGTTTGATTGTAACTg                                                                                                                                                                                                                                                            >  1:4620‑M2/38‑59 (MQ=255)                                                                                                                                                                                                            cTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCg                                                                                                                                                                                                             <  2:363030/103‑1 (MQ=255)                                                                                                                                                                                                            cTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCCGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCcgc                                                                                                                                <  2:216967/180‑1 (MQ=255)                                                                                                                                                                                                             aatcttcttcggcatcaggttaaggatcagaacTTTGACGGTTTGATTGTAACTGGTgc                                                                                                                                                                                                                                                        <  2:4620‑M2/26‑1 (MQ=255)                                                                                                                                                                                                              aCTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGGGGTCGAAAGATCACGTCACCTCGACGCTGCTTGTCTGCTGGGCGTTACAGGCCGCGCTCAATATCTTCTCCGCCATTCCTAAGC‑AAACTCGCCCCGACAAACTCTCTGGCGTTTATGAGCATCATATTCCCCATCCTCAAGCGCTTCTGAcg                              >  1:604621/1‑275 (MQ=255)                                                                                                                                                                                                                  tAACTTTGAAGATATTCAGGATCAGAACTTTGACGGtt                                                                                                                                                                                                                                                                        <  2:478069/38‑1 (MQ=255)                                                                                                                                                                                                                  tAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATc                                                                                                                                                                                                       >  2:327916/1‑103 (MQ=255)                                                                                                                                                                                                                      tttGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAAc                                                                                                                                                                                                   <  1:327920/103‑1 (MQ=255)                                                                                                                                                                                                                               atTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCt                                                                                                                                                                                                                                            >  1:427078/1‑53 (MQ=255)                                                                                                                                                                                                                                ttCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGt                                                                                                                                                                                       >  2:124822/1‑105 (MQ=255)                                                                                                                                                                                                                                ttCAGGATCAGAACTTTGAAGGTTTGATTGTAACGGGTGAGCCGCTGGGCCTGGTGGAGGTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCNTAAGC‑AAANGACAACCGAAAAACTATCTGGCGTTTACGAGAATCATATTCTCCATCCTCATGCGCTTCTGa                                >  1:467357/1‑255 (MQ=255)                                                                                                                                                                                                                                   aggatcagaacTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCtggtg                                                                                                                                                                                                                                        <  2:427073‑M2/42‑1 (MQ=255)                                                                                                                                                                                                                                    ggatcagaacTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTc                                                                                                                                                                                   <  1:124823‑M2/95‑1 (MQ=255)                                                                                                                                                                                                                                          gaacTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCAATCCTAAGC‑AAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCAc  >  1:424279‑M2/5‑275 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                TTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGC‑AAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCAC  >  CP009273/4204268‑4204775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.