| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 14,485:1 | +G | 71.8% | coding (318/1131 nt) | dnaJ → | chaperone Hsp40, DnaK co‑chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 14,485 | 1 | . | G | 71.8% | 92.0 / 39.8 | 39 | coding (318/1131 nt) | dnaJ | chaperone Hsp40, DnaK co‑chaperone |
| Reads supporting (aligned to +/- strand): ref base . (6/5); new base G (15/13); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
GCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑GGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGAC > CP009273/14252‑14712 | gCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGCCGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCtat > 1:334835/1‑274 (MQ=255) gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGGGGTGCTGATTTACGCTATAACATg > 1:7372/1‑275 (MQ=255) ggACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATCTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCTCGCGGTGCTGATTTACGCTATAACATGTAGCTCACCCTc > 1:424411/1‑275 (MQ=255) aCCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑g < 1:797992/216‑1 (MQ=255) ccGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTgcggcg > 2:790303/1‑210 (MQ=255) ggCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGcgcgcg < 1:790309/210‑1 (MQ=255) aaGAGAGCAAGGACGATTATGTAGTTCTGACCGACTCTCAAAAACGTGCGGCATACGATCAGTATGTTCATGCTGCGTTTGAGGAAGGTGGNATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGCCGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGCAGTGACCATAGAGATCCGCATTcc < 1:569325/275‑1 (MQ=255) aGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGCCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATGACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGAGAACAAAGAGAACAGAATTCCGAAGCAGGAagag > 1:75535/1‑275 (MQ=255) tGAAGTTATGACCGAATCGCAAAAACGTGCGGCATACGATCAGTATGGTAATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGgcgcg < 1:416393/178‑1 (MQ=255) cATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑GGTCGTCAACGTgcgg > 2:257872/1‑141 (MQ=255) cGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑GGTCGTCAACGTGCGgcgcg < 1:257875/141‑1 (MQ=255) aggctgcGTNTTAGCAAGTTGGCATGGGGGGGGGCGGGTTTGGCGGCGGCGCTGGTTTCAGCGATATTTTTGGGGAGGTTTTCGGCGATATTTTTGGCTGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATGACATGGAGTTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGt < 2:75535/271‑1 (MQ=255) gctgcGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGc > 2:544517/1‑181 (MQ=255) ctgcGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATgg > 1:757901/1‑151 (MQ=255) ctgcGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑GGTCGTCAACGTGCGgcgcgc > 2:768616/1‑125 (MQ=255) ctgcGTTTGAGCAAGGTGGCATGGGCGACGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATCTTTTTGGCGTCGGACGT‑GGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACACTCGACGCAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGCAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTCCACAGCCGCAGACTTGTCCGAcc > 1:502397/1‑275 (MQ=255) cGTTTGAGCACGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATTGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGa < 1:544520/181‑1 (MQ=255) cGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACg > 2:732749/1‑113 (MQ=255) gTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTgcgg > 2:128195/1‑117 (MQ=255) gTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCt < 2:757895/151‑1 (MQ=255) gTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑ggg < 1:25103/103‑2 (MQ=255) gTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGACGATATTTTTGGCGGCGGACGT‑GGTCGTCAACGTGCGGCGCGCGGTg < 1:768622/125‑1 (MQ=255) tttGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGc > 2:17168/1‑126 (MQ=255) tttGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑GGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCgaag > 1:209596/1‑160 (MQ=255) tttGAGCAAGATGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑GGTCGTCAACGTGCGGCGCGCGGTGCTGa > 1:812763/1‑128 (MQ=255) tGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTgcg < 1:732755/113‑1 (MQ=255) gAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGgcgcg < 1:128196/117‑1 (MQ=255) aGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGTGGCGCAGACTTCTGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑GGTCGTCAACGTGCGGCGCGCGGTGCTGATTTa < 2:812756/128‑1 (MQ=255) aGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGAt < 1:17168/126‑1 (MQ=255) aGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑g < 2:124150/96‑1 (MQ=255) aGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑GGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGc < 2:209593/160‑1 (MQ=255) ggcggcggcGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGAtt > 2:186592/1‑113 (MQ=255) gcggcggTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACg < 1:186595/113‑1 (MQ=255) gggtttttGGAGGCGGGGGACAATTCGGCGGTCTTTTTGGTGNCGTTTTCGGCGATAGTTGTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGa < 2:517620/267‑1 (MQ=255) gTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTATGGTCAGGTg > 1:517623/1‑264 (MQ=255) ttGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑GGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGt > 2:491038/1‑140 (MQ=255) cggcggcgCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑GGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCg < 1:491041/140‑1 (MQ=255) tgATATTTGTGGTGACGTGTTCGGCGATATTTTGGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTAAGTGGCGTGACCAAAGAGATCCGCATTGCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCCTGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGAc < 1:381328/273‑1 (MQ=255) cGATATTTGTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGCAAGAGTGTGACGTTTGCCACg > 1:725791/1‑174 (MQ=255) tatTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCa > 1:545477/1‑96 (MQ=255) tttGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCt < 2:545474/96‑1 (MQ=255) cggACGTGGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCAccc > 1:172576/1‑63 (MQ=255) | GCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGCGGCGGACGT‑GGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGAC > CP009273/14252‑14712 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |