| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,379,108 | G→A | 20.0% | A245A (GCG→GCA) | ycjX → | DUF463 family protein, putative P‑loop NTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,379,108 | 0 | G | A | 20.0% | 35.7 / 5.3 | 20 | A245A (GCG→GCA) | ycjX | DUF463 family protein, putative P‑loop NTPase |
| Reads supporting (aligned to +/- strand): ref base G (8/8); new base A (2/2); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
GTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGC > CP009273/1378874‑1379266 | gTCGGCGAAATGGCAGATGATGAGTGAAGGTCTGGACCCGCTATCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGAGTGGACCGATTAGCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAg < 1:449222/274‑1 (MQ=255) tGTAAATAGCAGGGGCAGCACTTTATTCAGCCTGGGCGCTTTGTGTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAg > 1:609493/1‑132 (MQ=255) aGGGGCTGCACTTTATTCAGCCTGGGCTCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAg > 1:503770/1‑158 (MQ=255) aGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGCGCGCCCGCGCTGCACTTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCCAACTGGCACAGGCCGATAAGCATACCAATGCCGGAATGCTTCGCGAACGCTTTAATTCTTACTGCGAGAACGTGGTGAAGGTGTTCTATCAGAATCATTTTCTGCGCtttt > 2:514419/1‑221 (MQ=255) ataagaggcagaTTCAGCCTGGGCTCTTTGTCTTGGCGGTGGCTATGGCCGGTGCGCCCGCGCTGCGATTCTTCCCGTGGACGGATGTCGATACCTGGGGCGAGTCCAATCTGGGGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGc < 2:207185/263‑1 (MQ=255) gCTGCACTTTATTAAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGAGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCACAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCg < 1:514422/222‑1 (MQ=255) tgaaCTTTATTCAGCCTGGGCGCTGTTTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCa < 2:379253/112‑1 (MQ=255) gCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGc > 1:346561/1‑117 (MQ=255) gCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGc > 2:395886/1‑117 (MQ=255) tttATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGAt < 1:395889/117‑1 (MQ=255) tttATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGAt < 2:346558/117‑1 (MQ=255) tATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCACAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACt > 1:189369/1‑161 (MQ=255) cAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCACAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCga < 2:189366/161‑1 (MQ=255) gcgcTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGTGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTgcg > 2:310351/1‑129 (MQ=255) gcgcTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCCGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCcac > 1:207188/1‑248 (MQ=255) gcTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGgcg < 1:376906/92‑1 (MQ=255) tttGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAg < 1:310354/129‑1 (MQ=255) ttGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGc > 1:15188/1‑94 (MQ=255) ttGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCt > 2:256223/1‑168 (MQ=255) cTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGAt < 2:15188/94‑1 (MQ=255) cTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATaa < 1:256226/168‑1 (MQ=255) | GTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGC > CP009273/1378874‑1379266 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |