Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,008,466 A→G 7.5% T36A (ACC→GCC)  fliG → flagellar motor switching and energizing component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,008,4660AG 7.5% 156.4 / 3.5 53T36A (ACC→GCC) fliGflagellar motor switching and energizing component
Reads supporting (aligned to +/- strand):  ref base A (24/25);  new base G (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

AAAGACGAACAACTACAACAACGGCGCGCTAACCAACGTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGA  >  CP009273/2008225‑2008719
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                             
aaaGACGAACAAGTACCAGAACGGAGCGAAAACCTACGTCGGGGGGAAGAAGTCATGGGCCAGCGTATCCGTGGAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGACAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAg                                                                                                                                                                                                                               <  2:33399/274‑1 (MQ=255)
                      ggggcgcTAACCAACTTTTGGGGGCAGAAGTCATGCGCCATCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTAGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGAAGCTAACCGa                                                                                                                                                                                                        <  1:804288/272‑1 (MQ=255)
                        ggggcgaaCCAACGGCTGGGGGAAGAAGTCATGAGCAAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGGGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCACCTAACCGAtg                                                                                                                                                                                                      <  1:795107/269‑1 (MQ=255)
                          cgcTAACCAACGTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGAtgtg                                                                                                                                                                                                    >  1:115391/1‑275 (MQ=255)
                           gcTTACCAACGTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACg                                                                                                                                                                                                                                  <  1:18636/244‑1 (MQ=255)
                                agcgggTCGGGGGGCAGAAGGCGTGGGCCACCGGATCCGCGAAATGTCTGGTTACGATCCGCGGGTGGGGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCg                                                                                                                                                                                              <  2:115391/270‑1 (MQ=255)
                                               aGAAGTCATGAGCCAGCGTATCCGTGAAATGCCTGATAACGATCCGCGCGTGGTGGCGCTGGTCCTTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCCCCGATAAAAGCGTAATCCTGCTGATGACCATTGGCGAAGACCGGGGGGCAGAGGTGCTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCCAGCAGAACTACAACAAGCAGCTAACCGAGGCGCTCGCggggggggggggggg                                                                                                                                                                                >  2:675095/1‑261 (MQ=255)
                                                     cATGGGCCTGCGTATCCGTGAACTTTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAACGCGTCATCCTGCTGGTGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGGGTTTGAGCAAGAAGCTGaa                                                                                                                                                                         <  1:675101/275‑1 (MQ=255)
                                                                      gTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACaaa                                                                                                                                                                                                                                                               >  1:104000/1‑172 (MQ=255)
                                                                        gAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAata                                                                                                                                                      >  1:143003/1‑275 (MQ=255)
                                                                          aattgcagATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCt                                                                                                                                                                                                                                                           <  2:104000/165‑1 (MQ=255)
                                                                                             cgcgTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCACGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTg                                                                                                                                  >  1:66432/1‑274 (MQ=255)
                                                                                                gcggtggCGCTGTTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGNTGACCATTNGCGAAGACCGGGAGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCAGCACTGATTATCAACGCCAACGATTATCTGCGCt                                                                                                                              <  1:554135/273‑1 (MQ=255)
                                                                                                            gTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGCTCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTg                                                                                                                                                                           >  1:486823/1‑218 (MQ=255)
                                                                                                            gTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTc                                                                                                                   <  2:143002/274‑1 (MQ=255)
                                                                                                            gTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCCCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAg                                                                                                                                                                                     >  1:57357/1‑208 (MQ=255)
                                                                                                                        tGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCGGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTg                                                                                                                                                                                       >  1:733044/1‑194 (MQ=255)
                                                                                                                                     aTCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGAGAACGt                                                                                                                                                                                                                                      >  1:499959/1‑134 (MQ=255)
                                                                                                                                         tGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTc                                                                                                                                                                                                                                     >  1:481814/1‑131 (MQ=255)
                                                                                                                                         tGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTg                                                                                                                                                                                       >  2:636770/1‑177 (MQ=255)
                                                                                                                                         tGAGTAACCAGACAGGCACCGATAAAGTCTTCATGCTGCTGCTGAGCCATGGCGAAGTCTGGGCGGCAGAGGTGTTCAAGCACCTCTCCATGCGTGAAATACAAACCCTGAGCGCTGCACTGGCCAACGTCACg                                                                                                                                                                                                                                  <  2:499956/134‑1 (MQ=255)
                                                                                                                                             tAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGc                                                                                                                                                                                                                                 <  2:481811/131‑1 (MQ=255)
                                                                                                                                             tAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCa                                                                                                                                                                                   <  1:636776/177‑1 (MQ=255)
                                                                                                                                                ccTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTg                                                                                                                                                                                       >  1:532627/1‑170 (MQ=255)
                                                                                                                                                    aCAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCa                                                                                                                                                                                   <  2:532624/170‑1 (MQ=255)
                                                                                                                                                    aCAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGc                                                                                                                                                                 >  1:282440/1‑188 (MQ=255)
                                                                                                                                                    aCAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCAGCCAGACTGCTGGAAGa                                                                          >  1:27137/1‑275 (MQ=255)
                                                                                                                                                     cAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTCTTGGTCAAAGCTCTGGGTGAAGAACGTGCCGACAGCATGCTGGAAGaa                                                                         >  1:388918/1‑274 (MQ=255)
                                                                                                                                                          aCCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAGCCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTa                                                                                                                                                                                                             >  2:156086/1‑138 (MQ=255)
                                                                                                                                                          aCCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAGCCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTa                                                                                                                                                                                                             >  2:694227/1‑138 (MQ=255)
                                                                                                                                                              aTAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAGCCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCg                                                                                                                                                                                                         <  1:694233/138‑1 (MQ=255)
                                                                                                                                                              aTAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAGCCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCg                                                                                                                                                                                                         <  1:156087/138‑1 (MQ=255)
                                                                                                                                                              aTAAAAGCGTCATCCTGCTGATGACCATTGGCCAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACTAGGAGCTAACCGATGTGCTGGCGGAGTTTGAGCa                                                                                                                                                                                   <  1:355817/160‑1 (MQ=255)
                                                                                                                                                                      gTCATCCTGCTGATGACCAATGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGa                                                                                                                                                                                                        >  1:679597/1‑131 (MQ=255)
                                                                                                                                                                          tCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGAtgtg                                                                                                                                                                                                    <  2:679591/131‑1 (MQ=255)
                                                                                                                                                                               cggatgcCCAGGGGCGAAGACCGGGCGGCAGAGGTGTACGAGCACCTCTCCCAGCGTGAAGTACAAACCCTGTGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGATGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAg                                               <  2:27137/273‑1 (MQ=255)
                                                                                                                                                                                    gaCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAATGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGt                                           >  1:469758/1‑274 (MQ=255)
                                                                                                                                                                                    gaCCATTGGCGAAGAACGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCATGAGCGCTGCAATGGCGAACGTCACGCAGATCGCCAACAAGCAGATAACCGATGTGCTGGCGGAGGTTGAGCAAGAAGCTGAACAGTTTGCCGCAATGAATAGCAACGACAACGATTATCTGCGCTCGGTATTGGTCCAAGCACTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGCTATTCTCGAAACTCACGATACCGCCAGAggga                                          >  1:378013/1‑273 (MQ=255)
                                                                                                                                                                                         ttGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGc                                                                                                                                                                 >  2:87060/1‑151 (MQ=255)
                                                                                                                                                                                             cGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCa                                                                                                                                                             <  1:87060/151‑1 (MQ=255)
                                                                                                                                                                                                 gACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTg                                                                                                                                                                                                                                                          >  2:529978/1‑54 (MQ=255)
                                                                                                                                                                                                     gggCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAgcg                                                                                                                                                                                                                                                      <  1:529981/54‑1 (MQ=255)
                                                                                                                                                                                                      ggcggcAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGt                                                                                                                                                                                                                                      >  2:639397/1‑69 (MQ=255)
                                                                                                                                                                                                          gcAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACg                                                                                                                                                                                                                                  <  1:639403/69‑1 (MQ=255)
                                                                                                                                                                                                              agGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGttt                                                                                                                                                                   >  1:483939/1‑128 (MQ=255)
                                                                                                                                                                                                                  gtTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCg                                                                                                                                                               <  2:483936/128‑1 (MQ=255)
                                                                                                                                                                                                                             tctcCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCACGCCTGCTGGCAGATATTCTCGAAACTCGCGATAACGCCAGCGGTATTGAAAGGATCAACCTTATGGAGCCCCAGAgctctatctt  >  2:259949/1‑266 (MQ=255)
                                                                                                                                                                                                                               tcCCAGCGTGAAGTACAAACCCTGGGCGCTGCAATGGCGAACGTCACGAAGAGCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTTCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACg                                                                                               <  1:335564/179‑1 (MQ=255)
                                                                                                                                                                                                                                 ccAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATg                      <  1:259952/250‑1 (MQ=255)
                                                                                                                                                                                                                                         aaGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGc                                                                                                                                                                 >  1:666195/1‑103 (MQ=255)
                                                                                                                                                                                                                                          aGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGcc                                                                                                                                                                >  1:355935/1‑103 (MQ=255)
                                                                                                                                                                                                                                          aGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACg                                                                                                                                          <  2:662459/125‑1 (MQ=255)
                                                                                                                                                                                                                                             aCAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCa                                                                                                                                                             <  2:666189/103‑1 (MQ=255)
                                                                                                                                                                                                                                              cAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCAc                                                                                                                                                            <  2:355932/103‑1 (MQ=255)
                                                                                                                                                                                                                                               aaaCCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGttt                                                                                                                                                                   >  2:527401/1‑95 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                             
AAAGACGAACAACTACAACAACGGCGCGCTAACCAACGTCTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCTCAACTTTATGGAGCCACAGAGCGCCGCCGA  >  CP009273/2008225‑2008719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑