| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,104,588 | G→T | 11.1% | L290L (CTC→CTA) | rfbD ← | dTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,104,588 | 0 | G | T | 11.1% | 118.5 / 4.0 | 41 | L290L (CTC→CTA) | rfbD | dTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase |
| Reads supporting (aligned to +/- strand): ref base G (20/16); new base T (2/3); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01 | |||||||||||
GGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACA > CP009273/2104382‑2104823 | gggTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTACATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGAAAAACCAAAAGTACTGCTGAAATTATTCtta > 1:295328/1‑273 (MQ=255) atcGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCgaga > 2:78346/1‑275 (MQ=255) gTAATAGCTGTTTACTGACATCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGc < 1:490451/274‑1 (MQ=255) aCTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAgg < 1:78346/275‑1 (MQ=255) cATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGC‑AGTAAATAATTCATTTAGCATTCGTTTAACGCCAACCTGCCAGt < 1:604101/182‑1 (MQ=255) tgtACCAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTg > 1:159117/1‑156 (MQ=255) tgtACCAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATa > 1:42250/1‑243 (MQ=255) tACCAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATa > 1:103557/1‑241 (MQ=255) ccAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAg < 2:159116/156‑1 (MQ=255) ccAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt < 2:42250/243‑1 (MQ=255) aGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt < 2:103557/241‑1 (MQ=255) gAACCACCTGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGc > 1:261064/1‑224 (MQ=255) caccTGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGgt < 2:261061/224‑1 (MQ=255) cccGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTTCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTCCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTTTTGGTACTGCGTTGAGCTTGTTGAGTGAAAGGGGaa > 1:401596/1‑274 (MQ=255) taCCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTgcg > 1:287684/1‑274 (MQ=255) aCCTTTACGCATTTTCATTCCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacgac > 1:116222/1‑200 (MQ=255) ccTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAACTTGCTGTAGT‑CGTAAATAATTCATTGAGCATTGGTTTAACGCCAACCTGCCAGTCAGGCAAGAAAAGCGCAACGTTCTGCTGAAATTTTTCTGTATTAAGGAGAGCGTTATGTggagga > 1:775427/1‑198 (MQ=255) ttACGCATTTTCATTCCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGc < 2:116222/200‑1 (MQ=255) tttcatttcaTCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGt > 2:265326/1‑122 (MQ=255) atttcatCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAgg < 1:265329/122‑1 (MQ=255) tttAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAtt > 1:181182/1‑147 (MQ=255) ttAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTCTCACGCCAACCTGCCAGTc > 2:624257/1‑104 (MQ=255) aTTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAgg < 2:181181/147‑1 (MQ=255) ttCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTCTCACGCCAACCTGCCAGTCAGGc < 1:624263/104‑1 (MQ=255) tCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGa > 1:41073/1‑68 (MQ=255) gCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCAt < 2:41073/68‑1 (MQ=255) accaTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacgac > 2:44258/1‑152 (MQ=255) tCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGc < 1:44258/152‑1 (MQ=255) aaCAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTTAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCt > 1:319942/1‑115 (MQ=255) aaCAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCt > 1:603511/1‑115 (MQ=255) aGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTTAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAt < 2:319939/115‑1 (MQ=255) aGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAt < 2:603505/115‑1 (MQ=255) aaaaCTATTAAATTGATG‑AGTGCGTAAAAAATTGCTTGAGCATTCTTTTCACTTCAACTTTGCAGTGAGGAAAGACAAGCGAAAAGTTCTGATGAAATTTTTCTGGATTAGGGCGAGGGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGGTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACa < 2:295325/273‑1 (MQ=255) aTTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATa > 2:281015/1‑142 (MQ=255) aaTTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt < 1:281018/142‑1 (MQ=255) cTGTAGT‑CGTAAATAATTCATTTAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtgtg > 2:207094/1‑111 (MQ=255) aGT‑CGTAAATAATTCATTTAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacg < 1:207097/111‑1 (MQ=255) ttCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGa > 1:108127/1‑115 (MQ=255) cATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtg > 2:309193/1‑120 (MQ=255) ttGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAgg < 2:108127/115‑1 (MQ=255) tGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATa > 2:460862/1‑112 (MQ=255) cgAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCaa > 1:719839/2‑150 (MQ=255) gAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgttgt < 1:309196/120‑1 (MQ=255) | GGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGT‑CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACA > CP009273/2104382‑2104823 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |