Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,848,801	G→T	9.8%	E369* (GAG→TAG)	fhlA →	formate hydrogenlyase transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,848,801	0	G	T	9.8%	141.6 / 2.1	47	E369* (GAG→TAG)	fhlA	formate hydrogenlyase transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (20/22);  new base T (3/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGTAATAATCGCCGCATGGTCAAAATGAACTGCGCGGCGATGCCTGCCGGATTGCTGGAAAGCGAT  >  CP009273/2848557‑2849043                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   cATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAATGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGGTGATGAAAACCTCGCCCTGACCGAGCAGCCCACCAATGTAGAAAGAGAAtta                                                                                                                                                                                                                       >  1:465305/1‑273 (MQ=255)       gttCGACACCTGGGGCAACCAGATTCAAACCTTTTGCCTGTTACCGCTGATGTTTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCTAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCg                                                                                                                                                                                                                   <  1:421750/272‑1 (MQ=255)            acacCTGGGGCAACCAGATTCAAACCTTGTGCCTATTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAACGTATTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCGTCGCCTATCAGGNAATCCCTCNTCTGAAAGAACGGCTGGTTGATGACAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGattat                                                                                                                                                                                                            >  1:565238/1‑274 (MQ=255)                    tgccaCCAGATTCAAACCTTGTGTCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAg                                                                                                                                                                                                   <  1:247816/271‑1 (MQ=255)                         gcgaaTTCCACCCGTGTGCCTGTTACCGCTGATTGCTGGCGACACAATGCTGCGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGaa                                                                                                                                                                                               <  2:103312/270‑1 (MQ=255)                                        gtgCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAAACTCGCCCTGACCGAGCAGCCACAAAACGTTAATAGTGAATTTGGCGAGAAAAAGGGACGCACCGAAACCATGTACaagagtc                                                                                                                                                                               >  2:808505/1‑269 (MQ=255)                                                        aTGTCTGGCGACACCATGCTGGGCGTGCTTAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTTAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCTCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACATAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGACGCAATGTAAAGCGAGCTTAAACAAGTTTAAAt                                                                                                                                                               >  2:761090/1‑275 (MQ=255)                                                            gtGGCGACACAATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGaa                                                                                                                                                                                               <  2:806465/238‑1 (MQ=255)                                                            cTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGTAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTgg                                                                                                                                                                                                                     <  1:375949/217‑1 (MQ=255)                                                                         tgtgtgagttgagAAACTGACGAAATACCAAGAGAAAGTGTTTACCAAGACCAATCTTAATTTACTACGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATAAGGAAGTCCATCGTCTGAAAGAACGGCTGGTTGATGATAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTTAATTTGGAGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGa                                                                                                                                                <  2:582432/261‑1 (MQ=255)                                                                         gCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCGCGCCTATGAGGAAATCCATCGTCTGAAAGAACGGCGGGTTGCTGAAAACCTCGCCCTGACCGAGCAGCTCAACAATCTTGATAGTTAAt                                                                                                                                                                                                                         >  2:75330/1‑200 (MQ=255)                                                                             ggCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCTCTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTgg                                                                                                                                                                                                                     <  1:75330/200‑1 (MQ=255)                                                                               cGTGCTGAAACTGGCGCAAAGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGCTGATGATAACCTCGCCCTGACCTAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGc                                                                                                                                                                                                  >  1:671032/1‑217 (MQ=255)                                                                                 tgcatataCTGGAGCAATGCGAAGAGACAGTGTTTACCACTACCAGTCTGACTTTCCTGCGCCAGATGGCAGAACGTGTGGCAATCGCTGTCGATAACGCCCGCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGag                                                                                                                                                                                                                                                  <  2:507754/160‑1 (MQ=255)                                                                                  gCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAACACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGAGATGTAAAGCGTGCTTAAACAAGTGGAAATGATGGCGCACAGTGACATTACCGTGc                                                                                                                                     >  2:362174/1‑275 (MQ=255)                                                                                   cTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACCATGTTGATAGTGAATTTGGCGAGATTATTGGCCGc                                                                                                                                                                                                     >  1:527478/1‑210 (MQ=255)                                                                                      aaaCTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGag                                                                                                                                                                                                                                                  <  2:438794/162‑1 (MQ=255)                                                                                                aaTGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGag                                                                                                                                                                                                                                                  <  1:145622/152‑1 (MQ=255)                                                                                                                           ccAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCATCGGTGAAAATGGCACGGGTAAAGAGTGGACTGcc                                                                                            >  2:728374/1‑275 (MQ=255)                                                                                                                              aTCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGAAAGACCCGGGCTGCACCACGGTGAAACTGGCACGGGTAAAGAANTGATTGCCCg                                                                                          >  2:68418/1‑274 (MQ=255)                                                                                                                               tCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGa                                                                                                                                                                                                                                                   >  1:145550/1‑120 (MQ=255)                                                                                                                                cTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGgc                                                                                                                                                         >  2:458838/1‑209 (MQ=255)                                                                                                                                 tggaTTTCCTGCGCCAGATTGCAGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTTAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCTAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGATGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCg                                                                                      <  1:761096/272‑1 (MQ=255)                                                                                                                                   aaTTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGagcag                                                                                                                                                                                                                                               <  2:145549/120‑1 (MQ=255)                                                                                                                                    aTTTACTGCGCCAGATTGCCGAACGTGTGGCGATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCaa                                                                                                                                                     <  1:458841/209‑1 (MQ=255)                                                                                                                                        aCTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGACCGGCTGGTTGATGAAAACCTCGCCATGACCGAGCAGCTCAACAATGTTGATAGCGATTGTGGCGCGATTATTGGCCGCATCGAAGACATGTACAGCGTGCTTAAACAAGCTGAAATGGCGGGGCAAAGTAACATGACGTTGCTGCTCCTCGGTGGAAATGGCACGGGGAACGCGCGGAGTGGCCGCGCGATGCaga                                                                               >  2:371141/1‑273 (MQ=255)                                                                                                                                              ccAGATTGCCGAACGGTTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCa                                                                         <  1:68418/275‑1 (MQ=255)                                                                                                                                                      ccGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGagc                                                                                                                                                                                                                                                 >  1:50695/1‑99 (MQ=255)                                                                                                                                                          aCGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCt                                                                                                                                                                                                                                             <  2:50695/99‑1 (MQ=255)                                                                                                                                                           cGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCTAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCg                                                                                                                                                                                                 >  1:336006/1‑142 (MQ=255)                                                                                                                                                           cGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGc                                                                                                                                                                                                     >  2:479699/1‑138 (MQ=255)                                                                                                                                                               tgGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCTAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGc                                                                                                                                                                                             <  2:336003/142‑1 (MQ=255)                                                                                                                                                               tgGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCg                                                                                                                                                                                                 <  1:479702/138‑1 (MQ=255)                                                                                                                                                                        cTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGAAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGACCGTGCGATCCa                                                                                 >  1:57513/1‑241 (MQ=255)                                                                                                                                                                                     ccTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGc                                                                                                                                                                                                     >  2:521533/1‑112 (MQ=255)                                                                                                                                                                                       tCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGagcag                                                                                                                                                                                                                                               >  2:450791/1‑68 (MQ=255)                                                                                                                                                                                       tCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAg                                                                                                                                                                                                   >  2:621022/1‑112 (MQ=255)                                                                                                                                                                                         gcttATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGc                                                                                                                                                                                                  >  2:640034/4‑111 (MQ=255)                                                                                                                                                                                         gCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCg                                                                                                                                                                                                 <  1:521536/112‑1 (MQ=255)                                                                                                                                                                                           cTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTca                                                                                                                                                                                                                                           <  1:450794/68‑1 (MQ=255)                                                                                                                                                                                           cTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGaa                                                                                                                                                                                               <  1:621028/112‑1 (MQ=255)                                                                                                                                                                                             aTCAGGAAATCCATCGTCTGAAATAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTGGGCGAGATTATTGGCCGCAGCGAAg                                                                                                                                                                                              <  1:640040/111‑1 (MQ=255)                                                                                                                                                                                                           cGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTCGAAATCGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGTAATAATCGCCGCATGGTCAAAATGAACTGCGCGGCGATGCCTGCCGGATTGCt            <  1:728380/275‑1 (MQ=255)                                                                                                                                                                                                                     aaCGGCTGGTTGTTTAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGTAATAATCGCCGCATGGTCAAAATGAACTGCGCGGCGATGCCTGCCGGATTGCTGGAAAGCGAt  <  1:362177/275‑1 (MQ=255)                                                                                                                                                                                                                                           cGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAg                                                                                                                                                                                                   >  2:211001/1‑60 (MQ=255)                                                                                                                                                                                                                                               cTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGaa                                                                                                                                                                                               <  1:211004/60‑1 (MQ=255)                                                                                                                                                                                                                                                  aCCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTg                                                                                                                   >  2:134532/1‑133 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   CATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAACAAGTTGAAATGGTGGCGCAAAGTGACAGTACCGTGCTGATCCTCGGTGAAACTGGCACGGGTAAAGAGCTGATTGCCCGTGCGATCCATAATCTCAGTGGGCGTAATAATCGCCGCATGGTCAAAATGAACTGCGCGGCGATGCCTGCCGGATTGCTGGAAAGCGAT  >  CP009273/2848557‑2849043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑