| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,213,182 | A→G | 10.7% | Q110Q (CAA→CAG) | patA → | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,213,182 | 0 | A | G | 10.7% | 92.4 / 2.0 | 35 | Q110Q (CAA→CAG) | patA | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (14/16); new base G (2/2); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAGGGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTAT > CP009273/3212918‑3213454 | gaAGCGAACGCTGGATCATGAGGAGATGAAAGTACTTATCCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGAGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAGGGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAaac < 1:768391/275‑1 (MQ=255) cTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGTGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAGGGCCAGGAGTTTAGCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGca < 1:59575/275‑1 (MQ=255) cTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCAGGAAGTTTAAATACGCTTGTCGACACCCAGGGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAAGAACCGCTGca < 1:798344/275‑1 (MQ=255) aTGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAGGGCCAGGAGTTTCTCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCCCCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACTGGCGATGTTTGCGAAAACCCTTTCTTCGCt > 1:301954/1‑274 (MQ=255) aTGTCAATCCGGGGTTTTTAGAGTATAGCAAATCGGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAGGGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGNCGTACAGANTCAACTTGCGAAACAACCGc > 1:550289/1‑213 (MQ=255) tAGAGTGGCAAGCGGGAAGATTAAATACGCTTGTCGACACCCAGGGGCAGGAGTTCACCGACTGCCTGGGAGGTTGTGGAATTTTCAACGTGGGGAACCGTAATCCAGTTGTGGTTTCCGCCGTACAGCATCAACATGCGGAACAACCGCTGcac > 2:198088/1‑155 (MQ=255) gTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAGGGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGcc < 1:198091/155‑1 (MQ=255) gCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAGGGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCAATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGAGGACCCGAGTCCGTTGAAGACGCACTGAAg > 1:476273/1‑274 (MQ=255) ggAAGTTTGAATACGCTTGTCGACACCCAGGGCCAGGAGGTTATCGACTGCCTGGTAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGc < 2:316023/139‑1 (MQ=255) ggAAGTTTAAATACGCTTGTCGACACCCAGGGCCAGGAGTTTATCGTCTGCCGGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGa < 1:345947/274‑1 (MQ=255) aaaTACGCTTGTCGACACCCAGGGTCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGTATTTTCAACGTGGGGCACCGTAATGCAGTTGTGGTTTACGCCGTACAGAATCAAATTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCACGGTAAACTGAAATACAGCTTCGTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTAc < 1:274033/274‑1 (MQ=255) ggAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTa > 1:350642/1‑275 (MQ=255) tttGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGc > 2:455308/1‑76 (MQ=255) tGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAAcc > 2:15714/1‑124 (MQ=255) gAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGca < 1:455311/76‑1 (MQ=255) aTTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTtg < 1:15714/124‑1 (MQ=255) ttCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAGCTTGCGAAACAACCGc > 2:337234/1‑67 (MQ=255) aCGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGca < 1:337237/67‑1 (MQ=255) cggggggCACCGTAATCCAGTTGTGGTTTGCGCCGGACAGAATCAGCATGCTCAACATCCGCTGCACAGCCAGGAGCGTCTAGTTCCGTTACGGGCGATGTTGGAGAAAGCCCGTGCTGCGCTAACGCCCGGTAAACTGGAATACAGCTTGTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAGATCACTTGgcg < 2:350639/272‑1 (MQ=255) ccgggggCACCGTCATCCAGTTGTGCTTTCGGCCGTACAGAATCAACTTGCGAAACAACCGAGGCCCAGCCAGGTGCTGTTCGGTCCGTTACGGTCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATCCAGCTTCTTCTGTAATAGCGGCACCGAGTCCGGTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGgcg < 2:803410/272‑1 (MQ=255) gTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTa > 1:434548/1‑80 (MQ=255) tAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGcac > 1:604188/1‑55 (MQ=255) tAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGt > 1:639171/1‑161 (MQ=255) tCCAGTTGTGGTTTCCGCCGTACAGAATCAGCTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTgg > 1:763015/1‑89 (MQ=255) tCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACggg < 2:434545/80‑1 (MQ=255) ccAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGcc < 2:604182/55‑1 (MQ=255) ccAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGt < 2:639165/161‑1 (MQ=255) gTTGTGGTTTCCGCCGTACAGAATCAGCTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGaa < 2:763009/89‑1 (MQ=255) gTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAgctgc > 1:246889/1‑55 (MQ=255) gTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGc > 2:792987/1‑138 (MQ=255) ccgccgTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGCGCTGCTCGa < 2:246886/55‑1 (MQ=255) ccgccgTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCa < 1:792993/138‑1 (MQ=255) cgTAAAAAATCACCTGGCCAAACACCCGCTGCACAGCCAGGTGCTCCGCGTTCTGTTTCGGGCTATGTTGCCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATGCAGCTTCTTCTGTAATAGCGGCACCTAGGCCGTTGAATCAGCGCGTAAGCTTGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACGTTTATTGCCAGCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCa < 2:651328/275‑1 (MQ=255) tCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGccc > 2:239697/1‑88 (MQ=255) cAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTCCCAGTCACCGCGCGGCAAGNTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTAt > 1:651334/1‑275 (MQ=255) aCTTGCGAAACAACCGGTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGAGGTTGGCGAAAACCCTTGCTGCGCTAACGcgc > 1:717396/1‑83 (MQ=255) aCTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAg > 2:455999/1‑102 (MQ=255) aCTTGCGAAACAACCGCTGCACAGCCAGCCGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGCGTCCgct > 1:649077/1‑131 (MQ=255) | GAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAGGGCCAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAACGCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTTACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTAT > CP009273/3212918‑3213454 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |