Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,360,812 C→A 8.2% L176L (CTC→CTA)  yhcF → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,360,8120CA 8.2% 154.1 / 2.0 51L176L (CTC→CTA) yhcFputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (25/21);  new base A (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

GTCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCAACTCCTGTAACGAACGCCATTATACCGCTTCAGGTGAGCGCATCTATCGCGAATGATTTAGGCGGTTCAGAAAAAATCGAACTTGATGGGAAAGCTGTGATAAGCGTAGTCTACTTGTGATAACAATGCCGGACAGGAGTACTATCAGGCATTCGTTTTATACGATCGGTTAATGTTTCAGGA  >  CP009273/3360589‑3361064
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                            
gTCAGAGTGGTGTAAAATCTACCTTAAATGGCGGAGGTTATATTAGACAGACAACGCTCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATACCGGTAGTTATGACGTGTTAATTGATAGCAACAACATTAGTTCCAGCAATGGTAGACAGACGTTAGCTGTATCAATAGCAGGTGCCGATGCTGTCATAACAGGGGAAAAAAGAGCATGGAAAACGCACAACGCTACACCGGTAGCAGCAGACCAAAGCAATGATTACACATTAGTaaa                                                                                                                                                                                                            >  2:260112/1‑274 (MQ=255)
            tAAAATATACCTTAAATGGCGGAGTTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAaac                                                                                                                                                                                                <  1:175897/274‑1 (MQ=255)
                    aCCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTGAGCTGTATCAATNGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCAAc                                                                                                                                                                                       >  1:700620/1‑275 (MQ=255)
                                ggAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAAGATCGGTAGTTATGCCGTGTNAATTGATANAAACAACATTAGCGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTACAACCAGGGGAAACAACCCCAACTCCTGTaacgaa                                                                                                                                                                           <  1:549526/275‑1 (MQ=255)
                                ggAAGTTATATTAGCCAGACACTGCGCTTATTCGGTTTAGGGAAAACAAAAGATAATAAAAATAGCGGTAGTTATGCCGTGTTAATTGATAGCAACAACATTAGTGCCAGCAATGGTAGCAAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCAACTCCTGTaacgaa                                                                                                                                                                           <  1:260115/275‑1 (MQ=255)
                                gaaGGTTATATAAGACAGACAACGCGCTTTTTCGGCTTAGGGAAAACAAAAGATAAGAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGCAACAACAGTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCAACTCCTGTaacgaa                                                                                                                                                                           <  2:700614/273‑1 (MQ=255)
                                              ccAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCACAGCTATTATTACACCTTAGTAAAACCAGGTGAAAAAACCACAAATCCTGTAACGAACACCATTATAccag                                                                                                                                                             >  1:129876/1‑273 (MQ=255)
                                                  aCAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCTATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAaacaa                                                                                                                                                                                              >  1:765268/1‑238 (MQ=255)
                                                      cgcgCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAAcccc                                                                                                                                                                                          <  2:765262/238‑1 (MQ=255)
                                                                cGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCNTGTTAATTGATAGCAACAATATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCaa                                                                                                                                                                                        <  1:649767/230‑1 (MQ=255)
                                                                             aCAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTGATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGACACAACCACAAATCCTGTAACGAACACAAATATACCGCTTCAGGTGAGCGAATCTATCGAGAAGGaata                                                                                                                              >  1:241746/1‑272 (MQ=255)
                                                                                 aaGATAAGAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGCAACAACAATACTGCCAGCCATGGTAGCCAGACGTTAGCTGTATCAATAGCCGGTGCAGATGCCGTCATAACAGGGCAACAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTAAACCTTTGTAAAACCAGGTGAAACAACCCCAACTCCTGAAACGAACGCCATTATCCCGACTCAGGTTCGCTCATCTACCGCCaantcgttacgc                                                                                                                          >  2:327389/1‑265 (MQ=255)
                                                                                                          tGCCGTGTTAATTGATAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACGAGGTGAAACAACNCCAACTCCNGTAACGAACGCCATTATACCGCTTCAGGTGAGCGCATCTATCGCGAATGATTTAGGCGGTTCAGAAAAAATCGAACTTGATg                                                                                                 >  1:558397/1‑275 (MQ=255)
                                                                                                                 ttAATTGATAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTa                                                                                                                                                                                                              >  1:492673/1‑159 (MQ=255)
                                                                                                                 ttAATTGATAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCAACTCCTGTAACGAACTCCATTATACCGCTTCAGGTGAGCGCATCTATCGCGAATGATTTAGGCGGTTCAGAAAAAATCGAACTAGAAGGGAAAGc                                                                                          >  1:762868/1‑275 (MQ=255)
                                                                                                                     ttGATAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAAc                                                                                                                                                                                                          <  2:492670/159‑1 (MQ=255)
                                                                                                                         tAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCtt                                                                                                                                                                                                                                               >  2:750571/1‑118 (MQ=255)
                                                                                                                          aGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCtt                                                                                                                                                                                                                                               >  1:712654/1‑117 (MQ=255)
                                                                                                                             aacaacATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAg                                                                                                                                                                                                                                           <  1:750577/118‑1 (MQ=255)
                                                                                                                              acaacaTTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAg                                                                                                                                                                                                                                           <  2:712648/117‑1 (MQ=255)
                                                                                                                                    ttAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTAACCGc                                                                                                                                                                                                                                                         >  1:783710/1‑97 (MQ=255)
                                                                                                                                        tGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTAACCGCTTAt                                                                                                                                                                                                                                                     <  2:783704/97‑1 (MQ=255)
                                                                                                                                          ccAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCaa                                                                                                                                                                                                                                   >  1:699050/1‑113 (MQ=255)
                                                                                                                                            aGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGt                                                                                                                                                                                                                                          >  1:615140/1‑104 (MQ=255)
                                                                                                                                              cAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCt                                                                                                                                                                                                                               <  2:699044/113‑1 (MQ=255)
                                                                                                                                                aTGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGAt                                                                                                                                                                                                                                      <  2:615134/104‑1 (MQ=255)
                                                                                                                                                  ggTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGATTATCCGCTTGCAGTCGATCaa                                                                                                                                                                                                                                   >  2:725887/1‑105 (MQ=255)
                                                                                                                                                      gCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCt                                                                                                                                                                                                                               <  1:725893/105‑1 (MQ=255)
                                                                                                                                                        cAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTNTGTAAAACCAGGTGAAACAACCCCAACTCCTGTAACGAACGCCATTATACCGCTTCAGGTGAGCGCATCTATCGCGAATGATTTAGGCGGTTCAGAAAAAATCGAACTTGATGGGAAAGCTGTGATAAGCGTAGTCTACTTGTGATAACAATGCCGGa                                                    <  1:755057/274‑1 (MQ=255)
                                                                                                                                                           aCGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTg                                                                                                                                                                                                                                              >  2:657167/1‑85 (MQ=255)
                                                                                                                                                               tAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGt                                                                                                                                                                                                                                          <  1:657173/85‑1 (MQ=255)
                                                                                                                                                                aGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCAACTCCTGTAACGAACGCCATTATACCGCTTCAGGTGAGCGCATATAACGCGAATGATTTAGGCGGTTCAGAAAAAATAGAAATTGATGGGAAAGCTGTGATAAGCGGAGTCGAATTGAGAAAAAAATGc                                                        >  2:755051/1‑262 (MQ=255)
                                                                                                                                                                       tCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGt                                                                                                                                                                                                                                          >  2:403643/1‑77 (MQ=255)
                                                                                                                                                                          ataggatgggCAGATGCCGTCAAACCAGGGAAAAAAAGAGCATTGAAAACGCATACCGCTTAGACGCTTACAGTGGATCAAAACTATTAGTACACCTTTGTAAAACCAGGTGAAACACCCCCAACTCCTGTAACGAACCCCATTATACCGCTTCAGGTGAGCGCATCTATCGCGAATGATGTAGGCGGTTCATAAAAAATCGAACTTGAGGGGAAAGCTGTGATAAGCGTAGTCTACTTGTGATAACAATGCCGGACAGGAGTACTATCAGGCAt                                 <  2:762862/266‑1 (MQ=255)
                                                                                                                                                                           tAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGAt                                                                                                                                                                                                                                      <  1:403646/77‑1 (MQ=255)
                                                                                                                                                                           tAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCaa                                                                                                                                                                                                                                   >  2:8883/1‑80 (MQ=255)
                                                                                                                                                                            aGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCAACTCCTGTAACGAACGCCATTATACCGCTTCAGGTGAGCGCATCTATCGCGAATGATTTAGGCGGTTCAGAAAAAATCGAACTTGATGGGAAAGCGGAGATAAGCGTAGGCTACTTGGGATAACAATGACGGAAAGGAGGACTATAAGGCATTc                               >  2:200921/1‑275 (MQ=255)
                                                                                                                                                                               aGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTg                                                                                                                                                                                                                                              >  2:620461/1‑65 (MQ=255)
                                                                                                                                                                               aGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCt                                                                                                                                                                                                                               <  1:8883/80‑1 (MQ=255)
                                                                                                                                                                                ggTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGt                                                                                                                                                                                                               >  1:61271/1‑95 (MQ=255)
                                                                                                                                                                                 gTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCAACTCCTGTAACGAACGCCATTATACCGCTTCAGGTGAGCGCATCTATCGCGAATGATTTAGGCGGTTCAGAAAAAATCGAACTTGATGGGAAAGCTGTGATAAGCGTAGTCTACTTGTGATAACAATGCCGGAAAGGAGTACTATCAGGCATTCGtttt                          >  1:218767/1‑275 (MQ=255)
                                                                                                                                                                                 gTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTAACCGCTTATCCGCTTGCAGTCGATCaa                                                                                                                                                                                                                                   >  1:132146/1‑74 (MQ=255)
                                                                                                                                                                                   gCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGAAAACGCTCACCGCTTATCCGCTTGCAGt                                                                                                                                                                                                                                          <  1:620467/65‑1 (MQ=255)
                                                                                                                                                                                    cAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTaaaa                                                                                                                                                                                                           <  2:61271/95‑1 (MQ=255)
                                                                                                                                                                                     aGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTAACCGCTTATCCGCTTGCAGTCGATCAAAGCt                                                                                                                                                                                                                               <  2:132145/74‑1 (MQ=255)
                                                                                                                                                                                           cGTCATAACAGGGCAAAAAAGAGCATCGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAaacaa                                                                                                                                                                                              >  1:547071/1‑101 (MQ=255)
                                                                                                                                                                                               aTAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAAcccc                                                                                                                                                                                          <  2:547068/101‑1 (MQ=255)
                                                                                                                                                                                               aTAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCAACTCCTGTAACGAACGCCATTATACCGCTTCAGGGGACCGCATCTATGGCNAATGATTTAGGCGGTTCAGAAAAAATCGAACTTGATGGGAAAGCTGTGATAAGCGTAGTCTACTTGTGATAACAATGCCGGACAGGAGTACTATCAGGCATTCGTTTTATACGATCGGTTaa            >  1:692045/1‑275 (MQ=255)
                                                                                                                                                                                                         aacaacgggaaTGGGAAATGCTCAAGNTTTATCCCCTTGCAGTAGATCAAAGCTATTATTACAACTTTGTAAAACCAGGTGAAACAACCCCGACTCCTGTAACGAACGCCATTATACCGCTTCAGGTGAGCGCATGTATAGCGAATGATTTAGGCGGTTCAGAAAAAATAGAACTTGATGGGAAAGCTGTGATAAGCGTAGTCTACTTGTGATAACAATGCCGGACAGGAGTACTATCAGGCATTCGTTTTATACGATCGGTTAATGTTTCAGGa  <  2:558394/265‑1 (MQ=255)
                                                                                                                                                                                                                     ggCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCAACTCCTGTAACGAACGCCATTATACCGCTTCAGGTGAGCGCATCTATCGCGAATGATTTAGGCGGTTCAGAAAAAATCGAAATTGATGGGAAAGCTGTGATAAGCGTAGTCTACTTGTGATAACAAtaca                                                       >  2:439586/1‑207 (MQ=255)
                                                                                                                                                                                                                         aaCGCTCACCGCTTATCCGCTTGCAGTCGATCTAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCAACTCCTGTAACGAACGCCATTATACCGCTTCAGGTGAGCGCATCTATTGCGAATGATTTAGGCGGTTCAGAAAAAATCGAACTTGATGGGAAAGCTGTGATAAGCGTAGTCTACTTGTGATAACAATGCCGGAc                                                   <  1:439589/210‑1 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                            
GTCAGAGTGGTGTAAAATATACCTTAAATGGCGGAGGTTATATTAGCCAGACAACGCGCTTATTCGGCTTAGGGAAAACAAAAGATAATAAAAATATCGGTAGTTATGCCGTGTTAATTGATAGCAACAACATTAGTGCCAGCAATGGTAGCCAGACGTTAGCTGTATCAATAGCAGGTGCAGATGCCGTCATAACAGGGCAAAAAAGAGCATGGCAAACGCTCACCGCTTATCCGCTTGCAGTCGATCAAAGCTATTATTACACCTTTGTAAAACCAGGTGAAACAACCCCAACTCCTGTAACGAACGCCATTATACCGCTTCAGGTGAGCGCATCTATCGCGAATGATTTAGGCGGTTCAGAAAAAATCGAACTTGATGGGAAAGCTGTGATAAGCGTAGTCTACTTGTGATAACAATGCCGGACAGGAGTACTATCAGGCATTCGTTTTATACGATCGGTTAATGTTTCAGGA  >  CP009273/3360589‑3361064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑