| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,695,494 | C→A | 10.2% | T245T (ACG→ACT) | dppF ← | dipeptide transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,695,494 | 0 | C | A | 10.2% | 149.8 / 5.2 | 49 | T245T (ACG→ACT) | dppF | dipeptide transporter |
| Reads supporting (aligned to +/- strand): ref base C (22/22); new base A (2/3); total (24/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 | |||||||||||
TAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCAACCCGGCGGTGGATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGACAGCCCCAACTCCTGCTGCAAATCCATCATCAGATTCAGCACCTGCGCGCGCACTGAAACATCCAGCGCGGAAACCGGTTCATCGGCAATCACCACATCCGGGTCGAGCATCAGACCACGGGCGATGGCGATACGCTGACGCTGACCGCCGGAGAA > CP009273/3695225‑3695745 | tagcGCTGCGGATTTTCATCCTGATCTACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCAACCCGGCGGTGGATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTcc < 1:164341/272‑1 (MQ=255) cGGATTCTCATCCTGATCAACAGTAAAATAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCCGGGCCCGAAGCGCCGACGACCGCGGGCGTTGAAGCCGCAACCGGTCGGTGCATTCAGTGGGCTGGGTAGTTCGCCGCTGGGCTTGATGCGCGCGCGGCGATCGTCGGGGTTCAGGCGAGGCGTCGCTGCAAGTAGCGCCTGAGAGAACGGAGGGCGGGGGTTAGTGAGGAGCTGGTCTGTCGtggccgttgaat > 2:226072/1‑264 (MQ=255) ttCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCAACCCGGCGGTGGATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGc < 1:684393/275‑1 (MQ=255) tCCTGATCATGAGCAAAACAAGCGACCAGTTGTCAGCATTAGTCTTGTTGCGGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCAACCCGGCGGTGGATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGc < 1:448312/274‑1 (MQ=255) cTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGTCGTTGAAGGCGCAACCCGGCGGTGGATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGc < 1:426632/218‑1 (MQ=255) cTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCAACCCGGCGGTGGATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATaa < 1:226075/275‑1 (MQ=255) gtggtaGGGGCCGAAGCGGTGACGACAGTGGGTGTTGAAGGCGAACCCCGGCGTTGGATTCAGTGGGCTGGGTAGTTCCCCGCTGAGCTTGATGCGCTCGCGGCTATCGTCCGGGTTCANGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATaa < 1:716976/267‑1 (MQ=255) ccGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCAACCCGGCGGTGGATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGGGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAg > 1:286658/1‑195 (MQ=255) gTTGAAGGCGCAACCCGGCGGTGGATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGgag > 1:519231/1‑235 (MQ=255) gTTGAAGGCGCAACCCGGCGGTGGATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCGCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGCAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCCGCGGACCAGGTACATCACCATCACTTCAAAAGCAATGTGCTCCACCACCGACAGGACGTGGGAGATAAAACAATACAAAAGCCCCAACCCCTGCAGCAAAccc > 2:804853/1‑274 (MQ=255) aaGGCGCAACCCGGCGGTGGATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGACAGCCCCAACTCCTGCTGCAAATCcatca < 1:144496/274‑1 (MQ=255) cggTGGATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCa > 1:808410/1‑149 (MQ=255) ggATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCa < 2:808403/149‑1 (MQ=255) aGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCAc > 1:240517/1‑140 (MQ=255) ggCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAg < 2:240514/140‑1 (MQ=255) cTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGAAATGTGCTCcaccac > 1:29111/1‑170 (MQ=255) aGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTAGTTCCCTTCTCCAc > 1:481073/1‑116 (MQ=255) gCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGAc < 2:29111/170‑1 (MQ=255) tGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTAGTTCCCTTCTCAACGCAg < 2:481070/116‑1 (MQ=255) gATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTAGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCt > 2:462359/1‑155 (MQ=255) cgcTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTAGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTccac < 1:462362/155‑1 (MQ=255) gcTCACGGCGTTCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGACAGCCCCAACTCCTGCTGCAAATCCATCATCAGATTCAGCACCTGCGCGCGCACTGAAACATCCAGCGCGGAAACCGGTTCATCGGCa < 1:324537/275‑1 (MQ=255) cTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCgg > 1:23646/1‑112 (MQ=255) cTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATANAACAGCCCCAAATCCTGCTGCAAATCCATCATCAGATTCAGCACCTGCCCGCGCACTGAAAAATCAACCGCGACAACCAGATCATCAGCaa > 1:629455/1‑275 (MQ=255) tCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCATGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCCATGTGCTCCACTACCGACAGGTc > 1:786369/1‑164 (MQ=255) cggcgATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCa < 2:23646/112‑1 (MQ=255) ggcgATCGGCCGGGTTCAGGCGCGGCGTCGCTGAAAGGTGCGCCTGAGTGTACGGATGGCGCGGGTGATTGAAGATTTGGTCTTTAGTTCCCTTCTCCACGCAGCCGCCCAGGTACATCACCATCACGTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGACAGCCCCAACTCCTGCTGCAAATCCATCATCAGATTCAGCACCTGCGCGCGCACTGAAACATCCAGCGCGGAAACCGGTTCATCGGCAATcac < 2:357253/274‑1 (MQ=255) ttCAGGGGCGGCGTCGCGGAAAGTAGCGACTGAGTGTACGGATGTCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGACAGCCCCAACTCCTGCNGCAAATCCATCATCAGATTCAGCACCTGCGCGCGCACTGAAACATCCAGCGCGGAAACCGGTTCATCGGCAATCACCAAATCCGAGTCGa > 1:603030/1‑274 (MQ=255) gcgcGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGCAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGc > 1:247651/1‑117 (MQ=255) gcggcgTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTAcatcaccat < 1:554644/103‑1 (MQ=255) ggcgTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACg > 2:360519/1‑78 (MQ=255) tCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGc < 1:360522/78‑1 (MQ=255) tAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCAc > 1:268583/1‑63 (MQ=255) gcCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAg < 2:268580/63‑1 (MQ=255) aGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATa > 2:347771/1‑132 (MQ=255) tACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGAc < 1:347774/132‑1 (MQ=255) aTGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTc > 1:39307/1‑105 (MQ=255) aTGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGACAGCCCCAACTCCTGCTGCAAATCca > 1:429062/1‑152 (MQ=255) gcgcGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGACAGCCCCAACTCCTGCTGCa > 2:454484/1‑143 (MQ=255) gcgcGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGACAGCCCCAACTCCTGCTGAAAATCCATCATCAGATTCAGCACCCGCGCGCGCACTGAAACATACAGCCCGAAAACACGTTCATCGGCAATACCCACATCCGGCTCGAGACACAACCCACGGGCGATGGCGCGACGCTACCCCGGACCCCCgg > 2:754250/1‑275 (MQ=255) cgcgGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAg > 1:471914/1‑45 (MQ=255) cgcgGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTgg < 2:39307/105‑1 (MQ=255) cgcgGGTTATTGAAGAGTGTATCTTTCGTTCCCTTCGCAACGCAGCAGCCCAGGTACATACCAATCACTTCCTCAGCAATGTGCTCCCACACCGACATGTCGTGGGAAATAAAGACATAAGACATCCACAACTCCTGCTGCAAATCcatcat < 2:429059/152‑1 (MQ=255) gggTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGACAGCCCCAACTCCTGCTGCAAATc < 1:454487/143‑1 (MQ=255) ggTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGc < 2:471911/45‑1 (MQ=255) ggTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCt > 2:377830/1‑81 (MQ=255) ggTTAGTGAAGATTTTGTCTTTCTTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTACTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGACAGCCCCAACTCCTGCTGCAAATCCATCATCAGATTCAGCACCTGCGCGCGCACTGAAACATCCAGCGCGGAAACCGGTTCATCGGCAATCACCACATCCGGGTCGAGCATCAGACCACGGGCGATGGCGATACGCTGACGCTGAACGCCGGAGaa < 1:804860/274‑1 (MQ=255) aTTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTccac < 1:377833/81‑1 (MQ=255) tCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGACAGCCCCAACTCCTGCTGCAAATAcat > 1:316275/1‑124 (MQ=255) | TAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCAACCCGGCGGTGGATTCAGTGGGCTTGGTAGTTCACCGCTGAGCTTGATGCGCTCGCGGCGATCGTCCGGGTTCAGGCGCGGCGTCGCGGAAAGTAGCGCCTGAGTGTACGGATGGCGCGGGTTATTGAAGATTTGGTCTTTCGTTCCCTTCTCCACGCAGCGGCCCAGGTACATCACCATCACTTCATCAGCAATGTGCTCCACCACCGACAGGTCGTGGGAGATAAAGACATAAGACAGCCCCAACTCCTGCTGCAAATCCATCATCAGATTCAGCACCTGCGCGCGCACTGAAACATCCAGCGCGGAAACCGGTTCATCGGCAATCACCACATCCGGGTCGAGCATCAGACCACGGGCGATGGCGATACGCTGACGCTGACCGCCGGAGAA > CP009273/3695225‑3695745 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |