Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,707,297	C→A	100%	A348E (GCA→GAA)	srmB →	ATP‑dependent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,707,297	0	C	A	100.0%	65.8 / NA	22	A348E (GCA→GAA)	srmB	ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/10);  total (12/10)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGA  >  CP009273/2707044‑2707556                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                    cTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACATCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGAGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCa                                                                                                                                                                                                                                                >  1:256951/1‑275 (MQ=255)  ttgCAAACTGGCGGCGCTAAGCGGGTATCAACAACTGCTATCTCGAAGGTGGGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACTGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGAGCGCGCCGGTCGTAAAGGCACCGAAGTTTCGCTGGTGGAAGCCCa                                                                                                                                                                                                                                                <  1:412285/272‑1 (MQ=255)  tGGGAAACTGGCTGCGCGACGAGGGCATCAAAAAGTGCTATCTCGAAGGTGAGAGGGTACAGGGCAAGCGTAAAGAAGCGATCAAGTGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCa                                                                                                                                                                                                                                                <  1:143249/274‑1 (MQ=255)                                                                                                                                                                   aGCCACTTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAg                                                                                                                                                                                                                                                    >  2:285903/1‑109 (MQ=255)                                                                                                                                                                       aCGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCa                                                                                                                                                                                                                                                <  1:285914/109‑1 (MQ=255)                                                                                                                                                                        cGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCATGACCATct                                                                                                                                                                                                                                       >  1:254665/1‑117 (MQ=255)                                                                                                                                                                            tttAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCATGACCATctgctg                                                                                                                                                                                                                                   <  2:254656/117‑1 (MQ=255)                                                                                                                                                                                              cgcAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGGGCTGAGAAGAAAAAAGATAAAGAGAAAGAGAAGCCGCGGGGGaaaa                                                    >  2:206380/1‑274 (MQ=255)                                                                                                                                                                                              cgcAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGACa                                                                                                                                                  >  1:245502/1‑180 (MQ=255)                                                                                                                                                                                                  gTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCg                                                                                                                                              <  2:245493/180‑1 (MQ=255)                                                                                                                                                                                                        aTACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTa                                                                                                                                                                                  >  2:97010/1‑138 (MQ=255)                                                                                                                                                                                                            ttATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtga                                                                                                                                                                              <  1:97011/138‑1 (MQ=255)                                                                                                                                                                                                                        tATCGGACGTACCGCGCGCGCCGGCCGTAAAGGCACCGAAATTTCGATGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCAGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGAAGACCGTCAAGCCATCGAANAAAGTAGTNGCTAAACGTGCTGAGAAGAAAAAAGCAAAAGAGAAAGAGAAGACGCGGGTGAAAAAACGCCATCGCGACaaaaaaaaaaag                          >  1:348219/1‑270 (MQ=255)                                                                                                                                                                                                                                gTACCGCGCGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCATGACCATctgctgc                                                                                                                                                                                                                                  >  1:404376/1‑66 (MQ=255)                                                                                                                                                                                                                                gTACCGCGCGCGCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGa                                                                                                                                                                                                         >  2:210876/1‑91 (MQ=255)                                                                                                                                                                                                                                    cgcgcgcgCCGGTCGTAAAGGCACCGAAGTTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTggg                                                                                                                                                                                                                              <  2:404361/66‑1 (MQ=255)                                                                                                                                                                                                                                    cgcgcgcgCCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAagag                                                                                                                                                                                                     <  1:210885/91‑1 (MQ=255)                                                                                                                                                                                                                                        cgcgcCGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGt                                                                                                                                                                                                                        >  2:86831/1‑68 (MQ=255)                                                                                                                                                                                                                                           gcCGGTCGTAAAGGCAGCGAAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTNCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGAAAACCaa       >  1:380540/1‑274 (MQ=255)                                                                                                                                                                                                                                            ccGGTCGTAAAGGCACCGAAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGc                                                                                                                                                                                                                    <  1:86832/68‑1 (MQ=255)                                                                                                                                                                                                                                               ggcgaaaaGGAAACNAAATCTCGAGGGTGGAAGACCATGTACATCGGGTGCTGGGTAAAGTAGGCCGCGATATTGAAGAGCCAATTAAAGCTAGCGTTATTGATGAGTTACGCCCGAAAACGCGGGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGa  <  2:380528/270‑1 (MQ=255)                                                                                                                                                                                                                                                          aCCGAAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAg                                                                                                                                                                                                                      >  2:360346/1‑52 (MQ=255)                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                    CTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGA  >  CP009273/2707044‑2707556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑