Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,167,378 C→T 100% E460K (GAA→AAA)  parE ← DNA topoisomerase IV, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,167,3780CT100.0% 103.4 / ‑6.7 33E460K (GAA→AAA) parEDNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (16/16);  minor base G (0/1);  total (16/17)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCTCTTTCCCGAGATCAATACGGTAGAGCGGTGGCAGTGCGACGTAAACGTGACCGTGTTTCACCAACGCGCGGAAATGTTTTACGAACAAAGCGCAGAGCAGCGTGGCAATGTGCAGACCATCAGAGTCCGCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATC  >  CP009273/3167109‑3167646
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                            
cTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCTCTTTCCCGAGATCAATACGGTAGAGCGGTGGCAGTGCGACGTAAACGTGATCGTGTTTCACCAACGCGCGGAAATGTTTTACGAACAAAGCGCAGAGCAGCGTGGCAATGTGCAGACCATCAGAGTCCGCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCg                                                                                                                                                                                                                                                                         <  1:262046/275‑1 (MQ=255)
                                                                                                      tttCACCAACGCGCGGAAATGTTTTACGAACAAAGCGCAGAGCAGCGTGGCAATGTGCAGACCATCAGAGTCCGCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCgg                                                                                                                                                                                                                                                       >  2:221079/1‑191 (MQ=255)
                                                                                                          aCCAACGCGCGGAAATGTTTTACGAACAAAGCGCAGAGCAGCGTGGCAATGTGCAGACCATCAGAGTCCGCATCCTCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAaga                                                                                                                                                                                                                                                   <  1:221088/191‑1 (MQ=255)
                                                                                                                                                   cGTGGCAATGTGCAGACCATCAGAGTCCGCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACt                                                                                                                                                                                                                                                              >  2:49204/1‑139 (MQ=255)
                                                                                                                                                       gCAATGTGCAGACCATCAGAGTCCGCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTtcgt                                                                                                                                                                                                                                                          <  1:49204/139‑1 (MQ=255)
                                                                                                                                                                       cAGAGTCCGCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGaa                                                                                                                                                                                                                                                     >  1:406185/1‑128 (MQ=255)
                                                                                                                                                                       cAGAGTCCGCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGAc                                                                                                                                                                                                                                                >  2:408394/1‑133 (MQ=255)
                                                                                                                                                                           gTCCGCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAgaga                                                                                                                                                                                                                                                 <  2:406170/128‑1 (MQ=255)
                                                                                                                                                                           gTCCGCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTcc                                                                                                                                                                                                                                            <  1:408409/133‑1 (MQ=255)
                                                                                                                                                                            tCCGCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTAc                                                                                                                                                                                                                         >  2:74521/1‑151 (MQ=255)
                                                                                                                                                                              cGCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACt                                                                                                                                                                                                                                               >  1:68806/1‑127 (MQ=255)
                                                                                                                                                                               gCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAgg                                                                                                                                                                                                                                        >  1:241038/1‑133 (MQ=255)
                                                                                                                                                                                cATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAgg                                                                                                                                                                                                                                        >  1:236879/1‑132 (MQ=255)
                                                                                                                                                                                cATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCttt                                                                                                                                                                                                                     <  1:74522/151‑1 (MQ=255)
                                                                                                                                                                                  tCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTccc                                                                                                                                                                                                                                           <  2:68806/127‑1 (MQ=255)
                                                                                                                                                                                   ccGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGtt                                                                                                                                                                                                                                    <  2:241029/133‑1 (MQ=255)
                                                                                                                                                                                    cgcgAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGtt                                                                                                                                                                                                                                    <  2:236870/132‑1 (MQ=255)
                                                                                                                                                                                             aCAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTAcc                                                                                                                                                                                                                        >  1:405453/1‑135 (MQ=255)
                                                                                                                                                                                               aGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACg                                                                                                                           >  1:338951/1‑226 (MQ=255)
                                                                                                                                                                                                 aTTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTc                                                                                                                                                                                                                    <  2:405438/135‑1 (MQ=255)
                                                                                                                                                                                                   tttGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTa                                                                                                                       <  2:338939/226‑1 (MQ=255)
                                                                                                                                                                                                                      cTCAGATCGTCGCTGTCACGATCGATACCGATCGCTACCGAAATATCGTGCACTTGCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCATGTTTTAAGGATCTTACCTTTCAGTGGCATGTTCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGcac                                                   <  1:261157/275‑1 (MQ=255)
                                                                                                                                                                                                                               tcgCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTc                                                                                                                                                                                                                    >  1:11706/1‑105 (MQ=255)
                                                                                                                                                                                                                                   tGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTg                                                                                                                                                                                                                <  2:11706/105‑1 (MQ=255)
                                                                                                                                                                                                                                               tACCGATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATcgc                                                                                                                                                                                     >  1:118545/1‑120 (MQ=255)
                                                                                                                                                                                                                                                   gATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCgcgcgc                                                                                                                                                                                 <  2:118544/120‑1 (MQ=255)
                                                                                                                                                                                                                                                   gATCGCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTc                                                                                                                                 >  2:260060/1‑168 (MQ=255)
                                                                                                                                                                                                                                                       gCTACCGAAATATCGTGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTa                                                                                                                             <  1:260069/168‑1 (MQ=255)
                                                                                                                                                                                                                                                                      tGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGa                                                                                                                                        >  2:267522/1‑142 (MQ=255)
                                                                                                                                                                                                                                                                      tGCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCa                                                                                                  >  2:166568/1‑180 (MQ=255)
                                                                                                                                                                                                                                                                       gCACTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTAGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGCACAACTTTTTTGGCCGCACGCATACGGCGATGGGCGCTGTAAATCGCCAtc  >  1:217796/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                          cTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAg                                                                                                                                    <  1:267533/142‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cTTTCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCa                                                                                              <  1:166572/180‑1 (MQ=255)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                            
CTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCTCTTTCCCGAGATCAATACGGTAGAGCGGTGGCAGTGCGACGTAAACGTGACCGTGTTTCACCAACGCGCGGAAATGTTTTACGAACAAAGCGCAGAGCAGCGTGGCAATGTGCAGACCATCAGAGTCCGCATCCGCGAGGATACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATC  >  CP009273/3167109‑3167646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑