| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,826,195 | C→T | 8.7% | R568H (CGC→CAC) | yicI ← | putative alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,826,195 | 0 | C | T | 8.7% | 138.5 / 3.0 | 48 | R568H (CGC→CAC) | yicI | putative alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base C (22/21); new base T (2/2); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
TTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGGTGTGTCCAGCGACCTTCCGGCAGGTAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGTAACAATCGCCAC > CP009273/3825945‑3826459 | tttATGCCAGTGAATACCGTCGGGTTCATCGTTCTGACACAGGTGTGTCCAGCGACCTTCCGGCAGGTAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGaagaag < 1:274372/274‑1 (MQ=255) aTGCCTGCTACTCCCTTCGTGTTCATCGTTGTTCCACAGTTGTGTCCATCGACCTTCCGGCTGGTAGAACTGCACATCGCCCGCTTCTGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGGCGGTCAAGGTAATCACAATCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGAGACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGca < 1:170580/275‑1 (MQ=255) gCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGGTGTGTCCAGCGACCTTCCGGCAGGTAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGTGGCATTTCAGTTGCGTGAAGAAGCGcac > 2:171959/1‑274 (MQ=255) cGACTACCGTCGAGTTCATCGTTGTGCCACAGGTGTGTCCAGCGACCTTCCGGCAGGTAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATAGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCCCGAAACACATAACGCCGCCAGGGGCATTTCACTTGCGTGacgaagacaaccagcgc > 2:170575/1‑264 (MQ=255) tgCCACAGGTGTGTCCAGCGACCTTCCGGCAGGTAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGTGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCa < 1:171965/275‑1 (MQ=255) ccAGCGACCTTCCTGCAGGTANAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCgg < 1:391405/205‑1 (MQ=255) ccGCGTCAGTGAACGCCGGAGAACCCATCACGTTGTCGACTAACATGTATTGTCGGTCAAGGTAATCACAAGCAGGATCGTCCGGGAACTCCATCATCATGGCGCGCATCATCGGCGTACCCCGCGCGTTCGAACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGagag < 1:316802/274‑1 (MQ=255) acCGGCGCAACCATCACGTTGTCGCCTACGATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCTGCATTTCAGTTGCGTGACGAAGCGCACCNCATCACAGGa > 1:371846/1‑194 (MQ=255) acCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCCCGGCACACGATAAGCATTGCTACCGTGTAAACGGCTATGCATGGAGAGAAAACCAAACGCGCa > 1:24383/1‑275 (MQ=255) acCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAAcgcga > 1:224077/1‑273 (MQ=255) gTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATAACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGGATGTAAACGTgcg > 1:223155/1‑275 (MQ=255) ggTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCacac > 2:270053/1‑180 (MQ=255) tCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGAGACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGagag < 1:193269/218‑1 (MQ=255) aaGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATa < 1:270064/180‑1 (MQ=255) aaGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGc < 1:50119/99‑1 (MQ=255) cGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTgaa > 1:86552/1‑105 (MQ=255) aaCTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGaagaag < 2:86551/105‑1 (MQ=255) catcatcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTaaa > 1:174820/1‑166 (MQ=255) atcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGc < 2:174813/166‑1 (MQ=255) tGGCACGCATCATCGGCGTAACCCGCGCGTTCGCACGTGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTTAGTTGCGTGAAGAAGCGCACCACATCATAGGACTCACGAACGTAGGCCCACgtc > 1:390132/1‑129 (MQ=255) cccGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATcac > 1:78473/1‑102 (MQ=255) ccGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCacac < 2:390117/131‑1 (MQ=255) cGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAgcgc > 1:226642/1‑90 (MQ=255) catcatCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGa < 2:78472/102‑1 (MQ=255) catcatCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGGAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCACCGTACGTAAACGTGCGCCGGAGCGCGATATTCAAAACCGCCGAAACCGTGGCCCCAGCAACCAAAACCCTAAACGACAATAAAc > 2:203560/1‑271 (MQ=255) tcatcGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGcacca < 2:226633/90‑1 (MQ=255) cGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATcac > 1:108330/1‑92 (MQ=255) gTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGa < 2:108329/92‑1 (MQ=255) tACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGTGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAAcgc > 1:124425/1‑169 (MQ=255) tACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGa > 2:347078/1‑119 (MQ=255) tACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAACCGTGCGCCGGAGCGGTATTTTCAAAGCCGCTGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCACAgg > 1:263138/1‑275 (MQ=255) ccGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGTGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCAc < 2:124424/169‑1 (MQ=255) ccGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAg < 1:347090/119‑1 (MQ=255) cgcgcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTTTAAACGGCTATGGCTGGAGAGCAAACCAAACGTCCACCAGCGTTTGTAAACGNGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTAACACGCCAATAGAAAAACCACCGCGCAGGCTTTc > 1:393651/1‑275 (MQ=255) cgcgcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTaaa > 1:34283/1‑135 (MQ=255) gcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAgcgc > 2:42413/1‑69 (MQ=255) cgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGc < 2:34283/135‑1 (MQ=255) tCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGcacca < 1:42413/69‑1 (MQ=255) cGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCAtt < 1:203568/274‑1 (MQ=255) aGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTaaa > 1:350203/1‑105 (MQ=255) aTACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTaaa > 1:127338/1‑103 (MQ=255) aCGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGc < 2:350191/105‑1 (MQ=255) ggCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGc < 2:127337/103‑1 (MQ=255) gCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCCCGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTTAAAGGCCAATAAACAAACCACCGCACAGGCTTTCCGCCATTGATTCGTAGTTAGCGTAACACTCAcccc > 2:133585/1‑271 (MQ=255) atcatGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTaaa > 2:433593/1‑142 (MQ=255) tcatGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTa > 2:418778/1‑58 (MQ=255) catGCGGCATTTCAGTGGCGTGAAGAAGCGCACCACATCAGAGGACTAAACATCGTAGGCCGACGGCACACGATAAGATTTGCTACCGGGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACACACCACCGCGCAGGCTTTCCGCCATTGAt < 1:142383/244‑1 (MQ=255) tGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTCTAAACGTg < 1:433608/142‑1 (MQ=255) gCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGcc < 1:418793/58‑1 (MQ=255) | TTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGGTGTGTCCAGCGACCTTCCGGCAGGTAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACCAAACGCGCACCAGCGTTTGTAAACGTGCGCCGGAGCGGTATTTTCAAAGCCGCCGATATCGTGGCTCCAGAAGCCAAAACCTGAAAGGCCAATAGACAAACCACCGCGCAGGCTTTCCGCCATTGATTCGTAGTTAGCGTAACAATCGCCAC > CP009273/3825945‑3826459 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |