| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,476,880 | T→C | 15.7% | M107T (ATG→ACG) | yheT → | UPF0017 family putative hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,476,880 | 0 | T | C | 15.7% | 52.3 / 5.5 | 26 | M107T (ATG→ACG) | yheT | UPF0017 family putative hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (11/10); new base C (2/2); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.18e-01 | |||||||||||
AAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTGAAAACCCTGCACAGGCGCAACATAAACCTCGTCTGGTGGTGTTTCACGGGCTGGAAGGCAGTCTCAACAGCCCTTACGCCCACGGTCTGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTGGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTA > CP009273/3476684‑3477126 | aaatgtaCCCCGGTCTGGCAGCGGCGGGAGGTGCACGACGGCGATTTTGTCGGTCTTGCGTGGAGTAAAACCCCTGCACAGGCGCAACATAAACCGCGTCTGGTGGTGTTTCACGGGCTGGAAGGCAGTCTCAACATCCCTTACGCCCACGGTCTGGTTGAGGCGGAGCAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGtt < 1:39096/268‑1 (MQ=255) tGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTGAAAACCCTGCACAGGCGCAACATAAACCTCGTCTGGTGGTGTTTCACGGGCTGGAAGGCAGTCTCAACAGCCCTTACGCCCACGGTCTGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGc > 1:61382/1‑182 (MQ=255) cccGACGGCGATTTTGTCGATCTTGCGTGGAGTGAAAACCCTGCACAGGCGCAACATAAACCTCGTCTGGTGGTGTTTCACGGGCTGGAAGGCAGTCTCAACAGCCCATACGCCCACGGTATGGTTGCGGCGGCGCACAAGCGCGGCTGGCTGGGCGTGGTGATGAATTTCCGCGGATGCAGCNGTGAACCANACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTAGGTTATTACGCTGGCTGAAACGCGAATTTGGTc > 1:224570/1‑274 (MQ=255) cagATTTTGTCGATTTTGGGTGGAGTGAAAACCCTGCACAGGCGAAACATAAACCGCGTCTGGTGGTGTTTCACGGGCTGGAAGGCAGTCTCAACAGCCATTACGCCCACGGTCTGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGcc < 1:148749/272‑1 (MQ=255) cTTGCCGGGAGTGAAAACCCTGCGCAGGCGCAAAATAAACCTCGTCTGGTGGTGTTTCACGGGCTGGAACGCATTCTCAACAGCCCTTAAGCCGCCGGTCCTGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGAGGGGTGATCCATTTTCGGGAATGCACCGGTGACCCAAACCGGAACCAGCCAATTTACCATGCGGGCGCAACCGAAGACGCAAGTGGGTTTTGACGCTGGCGGCAACGCGAAATTGGTCCTTCGCCAACGTCTccctc > 2:148746/1‑270 (MQ=255) aGTGAAAACCCTGCACAGGCGCAACATAAACCTCGTCTGGTGGTGTTTCACGGGCTGGAAGGCAGTCTCAACCGCCCTTACGCCCACGGTCTGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGACAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGc > 1:122250/1‑274 (MQ=255) aGTGAAAACCCTGCACAGGCGCAACATAAACCTCGTCTGGTGGTGTTTCACGGGCTGGAAGGCAGTCTCAACAGCCCTTACGCCCACGGTCTGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGACGCATTTTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACg > 2:100831/1‑205 (MQ=255) ccaactccgCACCGGCGCCACAGCANACGCGTGGGGTGGTGTGTCACGGGCGTGAAGTCAGGCTCAACCGTCGTTACGCTCACGGGTTGTTTTAGGCGGCGCAAAAGCGCGGCTGGCTGGGAGTGGTGAGGCATTTGCGCGGATGCAGCGGTGAACCTATCCGTATGCACCGCATTTACCCTGCGGGCGAAACCGAAGACGCCTGTTGGTTTTTACGCTGTCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCTGCTATTCGCTcgg < 2:224564/266‑1 (MQ=255) aaaaCCCTGCACAGGCGCAACATAAACCTCGTCTGGTGGTGTTTCACGGGCTGGAAGGCAGTCTCAACAGCCCTTACGCCCACGGTCTGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGACGCATTTTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAg < 1:100833/205‑1 (MQ=255) ccTGCACAGTCGCACAATAATCCTCGTCTGGTGTTGTTTCACGGGCTGGAAGGCAGTCTCAACAGCCCTTACGCCCACGGTCTGGGTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGTATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTa < 1:46801/274‑1 (MQ=255) gTCTGGTGGTGTTTCACGGGCTGGAAGGCAGTCTCAACAGCCCTTACGCCCACGGTCTGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGACGCATTTTCGCGGATGCAg > 1:14008/1‑118 (MQ=255) ggtggtGTTTCACGGGCTGGAAGGCAGTCTCAACAGCCCTTACGCCCACGGTCTGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGACGCATTTTCGCGGATGCAGCGGt < 2:14008/118‑1 (MQ=255) ggAAGGCAGTCTCAACAGCCCTTACGCCCACGGTCTGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCAtt > 1:51784/1‑131 (MQ=255) ggCAGTCTCAACAGCCCTTACGCCCACGGTCTGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCggg < 2:51783/131‑1 (MQ=255) cGCCCACGGTCTGGTTGAGGCGGCGCAAAAGCGCGGNTGGCTGGGNGTGGTGATGCATATTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGACGTCGGCTATTCGCGCGGCGGTGATATGCTGGCCTGTTTGCTGGCAaag < 1:224298/229‑1 (MQ=255) ggTCTGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCa > 1:63582/1‑64 (MQ=255) tGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCAGCgg < 2:63580/64‑1 (MQ=255) aGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTGGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGc > 1:99176/1‑274 (MQ=255) aGGCGGCGCAAAAGCGCGCCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCAGCGGGGCACCAAACGTTCTGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTCTTACTCTGGCCGCAACGCGAATTTGCTCCTGCGCCAACGGCGGCCGTCCGCTATTCCCTCGGCGGTAACATGCTGCCCTGGTGGTAGGAGAAGCGAGAGAATGATCTCCCACTAATTACGGCGGGGAGTGGCTCTGCGTCGTTTATGCTggcggct > 2:315527/1‑270 (MQ=255) ggggcAAAGGGGCGGCTGGCTGGGCGTGGTTATGCATTTTGGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTGGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGt < 2:99174/271‑1 (MQ=255) cgcAAAAGCGCGGCTGGCTGTGCGTGGTGATGCATTTTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTGGCCTGTTGGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGc > 1:329990/1‑275 (MQ=255) cgcAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGc > 2:185332/1‑47 (MQ=255) cAAAAGGGGGGGTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATGCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTGGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTa < 1:131510/275‑1 (MQ=255) aaaGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCAGcc < 1:185336/47‑2 (MQ=255) gCGTGGTGATGCATTTTCGCGGATGCAGCGGTGaa > 1:9595/1‑35 (MQ=255) ggtgATGCATTTTCGCGGATGCAGCGGTGAACCaa < 2:9595/35‑1 (MQ=255) | AAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTGAAAACCCTGCACAGGCGCAACATAAACCTCGTCTGGTGGTGTTTCACGGGCTGGAAGGCAGTCTCAACAGCCCTTACGCCCACGGTCTGGTTGAGGCGGCGCAAAAGCGCGGCTGGCTGGGCGTGGTGATGCATTTTCGCGGATGCAGCGGTGAACCAAACCGTATGCACCGCATTTACCATTCGGGCGAAACCGAAGACGCCAGTTGGTTTTTACGCTGGCTGCAACGCGAATTTGGTCATGCGCCAACGGCTGCCGTCGGCTATTCGCTCGGCGGTAATATGCTGGCCTGTTTGCTGGCAAAGGAAGGCAATGATCTCCCGGTTGATGCGGCGGTGATTGTCTCTGCGCCGTTTATGCTGGAAGCCTGTAGCTA > CP009273/3476684‑3477126 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |