Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,872,089 G→T 100% S464Y (TCT→TAT)  gyrB ← DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,872,0890GT100.0% 80.1 / ‑6.7 28S464Y (TCT→TAT) gyrBDNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (14/13);  minor base A (1/0);  total (15/13)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCG  >  CP009273/3871823‑3872317
                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                    
cccgcttttCGTCGTTTTTAATGTACTGTTCCTGCTTGCCTTTCTTCGCTTTGTACCGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATGTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAagagag                                                                                                                                                                                                                               <  2:43370/267‑1 (MQ=255)
   tCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGACGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAAGACCGATACCACAGCCAAGAGCGGTGATAAGCGTCGCCACATACTCAAAAGAGAACATc                                                                                                                                                                                                                           >  1:173808/1‑275 (MQ=255)
                            ttCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCGGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTACGGGTAGTACTCGTCACGACCGATACCACAGCCAAGCGAGGTGAGAAGCGTCGCCACTACATGATAAGAAAGCATCTTATCGAAGAGCGCTAACTCGCCGt                                                                                                                                                                                                  >  1:17132/1‑275 (MQ=255)
                             tCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGtt                                                                                                                                                                                                 <  1:29741/275‑1 (MQ=255)
                                                           ggcggcTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGAAGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTAATCGAAGCGCGCTTCATCACCGTTGAGGCCTTTATCCTTCAGCGTCAGacgcg                                                                                                                                                                    >  1:12598/1‑270 (MQ=255)
                                                                                  aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTACGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAACGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGAGACAGAATAGACTGGGTCTTGCGGTTACGcccc                                                                                                                                             >  1:43016/1‑274 (MQ=255)
                                                                                                                                               tgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCa                                                                                                                                    >  2:281425/1‑222 (MQ=255)
                                                                                                                                               tgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCa                                                                                                                                    <  1:281436/222‑1 (MQ=255)
                                                                                                                                                 cGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTGATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTCTCGGTTACGCCACTGCTTCGCCGAGCCGCCCGAGGCGGCCCCTTCCCCCAGGCAAGGTTCGGacccgcgcggcagc                                                                              >  2:75129/1‑261 (MQ=255)
                                                                                                                                                              tCCGCATCGGTCATGATGATGATGCTGTGATAACGGAGTGTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTc                                                                <  1:75131/275‑1 (MQ=255)
                                                                                                                                                                                               cgtaGTTTTTCCGGGTTGTACTCGTCACGTCCGATACCACAGCCAAGCGCGGTGATATGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAg                               <  1:58756/272‑1 (MQ=255)
                                                                                                                                                                                               cGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTgg                                                                                                                                                               >  2:212485/1‑147 (MQ=255)
                                                                                                                                                                                               cGCAGTTTGTCCGGGGTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCNCGGAGTCCCCTTCCACCAGGTCCAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCAAGTTTGCCCGGCCGGCCCgatccaccaca                               >  1:239538/1‑265 (MQ=255)
                                                                                                                                                                                                gCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGataaga                                                                                                                                                                                                                                  <  1:104759/79‑1 (MQ=255)
                                                                                                                                                                                                  aGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAgcg                             <  1:213681/274‑1 (MQ=255)
                                                                                                                                                                                                   gTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCt                                                                                                                                                           <  1:212491/147‑1 (MQ=255)
                                                                                                                                                                                                    tttGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTcc                                                                                                                  >  2:27955/1‑187 (MQ=255)
                                                                                                                                                                                                        tCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCtt                                                                                                              <  1:27955/187‑1 (MQ=255)
                                                                                                                                                                                                            ggTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAagagag                                                                                                                                                                                                                               >  1:238130/1‑70 (MQ=255)
                                                                                                                                                                                                                gTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATc                                                                                                                                                                                                                           <  2:238123/70‑1 (MQ=255)
                                                                                                                                                                                                                      gTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCa                                                                                                                                                                                >  2:310526/1‑107 (MQ=255)
                                                                                                                                                                                                                      gTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAgccgcc                                                                                                                            >  2:122987/1‑159 (MQ=255)
                                                                                                                                                                                                                          cGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCgg                                                                                                                                                                            <  1:310537/107‑1 (MQ=255)
                                                                                                                                                                                                                          cGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCcgcg                                                                                                                        <  1:122989/159‑1 (MQ=255)
                                                                                                                                                                                                                             ccGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGATCCGCCAGCGGAGTCCCCTTCCACCAGGTACCGTTCGGAAACCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTAAGGCGGGTCCTTTCAcgcg  >  1:299618/1‑274 (MQ=255)
                                                                                                                                                                                                                                       aGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGt                                                                                                                                                                                                  >  1:143339/1‑72 (MQ=255)
                                                                                                                                                                                                                                         ccAAGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCg                                                                                                                                                       >  2:49748/1‑113 (MQ=255)
                                                                                                                                                                                                                                           aaGCGCGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAg                                                                                                                                                                                              <  2:143336/72‑1 (MQ=255)
                                                                                                                                                                                                                                             gcgcGGTGATAAGCGTCGCCACTTCCTGATAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTa                                                                                                                                                   <  1:49749/113‑1 (MQ=255)
                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                    
CCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCG  >  CP009273/3871823‑3872317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑