| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,159,093 | G→T | 100% | S80R (AGC→AGA) | parC ← | DNA topoisomerase IV, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,159,093 | 0 | G | T | 94.3% | 37.6 / ‑5.4 | 16 | S80R (AGC→AGA) | parC | DNA topoisomerase IV, subunit A |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (7/8); minor base A (0/1); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGTCAGCCGTCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGTAAGAGAACGGTTGCGCCATCAGGACCATCGCTTCATAACAGGCGCTATCGCCGTGCGGATGGTATTTACCCAGTACGTCACCGACGGTACGGGCCGATTT‑TTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCGTTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCC > CP009273/3158906‑3159359 | aGTCAGCCGTCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGTAAGAGAACGGTTGCGCCATCAGGACCATCGCTTCATAACAGGCTCTATCGCCGGGCGGCTGGTATTTACCCAGTACGTCACCGACGGTACGGGCCGCTAT‑TTTAAATGTGGCGCTGGCATTCAGGCCCAg > 1:18213/1‑274 (MQ=255) tCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGTGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGACCATCANCCAGCGGATAACGGTAAGAGAACGGTTGCGCCATCAGGACCATCGCTTCATAACAGGCTCTATCGCCGTGCGGATGGTATTTACCCAGTACGTCACCGACGGTACGGGCCGATTT‑TTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGAc < 1:286476/272‑1 (MQ=255) cTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGTAAGAGAACGGTTGCGCCATCAGGACCATCGCTTCATAACGAGCTCTATCGCNCTGCGGATGGTATTTACCCAGTACGTCACCGACGGTACGGGCCGATTT‑TTTAAATTTTGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGtt > 1:309718/1‑275 (MQ=255) tgtaaTATTTCGACCTACGGGATTCTGTGTAACGCATTGCCGCGTACGATTTCGGATCGTCCTGCGCGCCCCAGTTCCCGTTACCATCAACCAGCGGATAACGGTAATAGAACGGTTGCGCCATCAGGACCATCACTTCATAACAGGCTCTATCGCCGTGCGTATTGTATTTACCCAGTACGTCACCGACGGTACGGGCCGATTT‑TTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTAAACAGGTTTc < 1:334023/272‑1 (MQ=255) ggtAACGCACTCCCGCGACCCATGTGCGAGCGGCCGGTGCGCCCACGTTCCCCGGTCCAGATACGAGCAAAGAACGGTAAGAGAACTGTGGCGCCAGCAGGACCCGCACTAAATATCATGCACTAGCGACGTGCTGATGGTAGTTACGCAGAACGTCACCGACTGTACGGG‑CGATTTCTTCAGGTTTGGCCAAGGCGTTGGGGAACACATCAGACATCGGACGAACGATGCAGCGCTGACCATGTTGCAGACCATCACCAATAAACGGCAAGGCa < 2:309707/274‑1 (MQ=255) tCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGTAAGAGAACGGTTGCGCCATCAGGACCATCGCTTCATAACAGGCTCTATCGCCGTGc > 1:45108/1‑102 (MQ=255) tCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGTAAGAGAACGGTTGCGCCATCAGGACCATCGCTTCATAACAGGCTCTATCGCCGTGCGGATGGTATTTACCCAGTACGTCACCGACGGTACGGGCCGATTT‑TTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCaa > 1:142635/1‑239 (MQ=255) gcgcgcCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGTAAGAGAACGGTTGCGCCATCAGGACCATCGCTTCATAACAGGCTCTATCGCCGTGCGGAt < 2:45107/102‑1 (MQ=255) gcgcgcCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGTAAGAGAACGGTTGCGCCATCAGGACCATCGCTTCATAACAGGCTCTATCGCCGTGCGGATGGTATTTACCCAGTACGTCACCGACGGTACGGGCCGATTT‑TTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCa < 2:142632/239‑1 (MQ=255) tgATAACGGTAAGAGAACGGTTGCGCCATCAGGACCATCGCTTCATAACAGGCTCTATCGCCGTGCGGATGGTATTTACCCANTACGTCACNGACGGTACGGGCCGATTT‑TTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGGAGGCGTTTTCCGTAAATTCATGTAGCGCAAGgcg < 1:223127/274‑1 (MQ=255) cGGTTGCGCCATCAGGACCATCGCTTCATAACAGGCTCTATCGCCGTGCGGATGGTATTTA‑CCAGTACGTCACCGACGGTACGGGCCGATTT‑TTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACaa > 1:558/1‑142 (MQ=255) gTTGCGCCATCAGGACCATCGCTTCATAACAGGCTCTATCGCCGTGCGGATGGTATTTACCCAGTACGTCa > 2:42241/1‑71 (MQ=255) tGCGCCATCAGGACCATCGCTTCATAACAGGCTCTATCGCCGTGCGGATGGTATTTA‑CCAGTACGTCACCGACGGTACGGGCCGATTT‑TTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATgcg < 2:558/142‑1 (MQ=255) cgcCATCAGGACCATCGCTTCATAACAGGCTCTATCGCCGTGCGGATGGTATTTACCCAGTACGTCACCGa < 1:42242/71‑1 (MQ=255) cgcCATCAGGACCATCGCTTCATAACACGCTCTATCGCCGTGCGGATGGTATTTACCCAGTACGTCACCGAc > 1:195457/1‑72 (MQ=255) aaCAGGCTCTATCGCCGTGCGGATTGTATTTACCCAGTACGTCACCGACGGTACGTGCCGATTT‑TTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCGTTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGcc < 1:178036/274‑1 (MQ=255) | AGTCAGCCGTCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGTAAGAGAACGGTTGCGCCATCAGGACCATCGCTTCATAACAGGCGCTATCGCCGTGCGGATGGTATTTACCCAGTACGTCACCGACGGTACGGGCCGATTT‑TTTAAATTTGGCGCTGGCATTCAGGCCCAGTTCAGACATCGCATACACAATGCGGCGCTGAACAGGTTTCAGACCATCACCAATAAACGGCAACGCACGGTCCATGATCACGTACATGGAGTAGTTTAAGTAGGCGTTTTCCGTAAATTCATGTAGCGCAAGGCGCTCTGCCATATCGCTCATTAATTCTGATTCCTCAACTTATTCGCC > CP009273/3158906‑3159359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |