| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,379,843 | C→G | 12.5% | A569P (GCG→CCG) | aaeB ← | p‑hydroxybenzoic acid efflux system component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,379,843 | 0 | C | G | 12.5% | 89.9 / 5.9 | 32 | A569P (GCG→CCG) | aaeB | p‑hydroxybenzoic acid efflux system component |
| Reads supporting (aligned to +/- strand): ref base C (17/11); new base G (2/2); total (19/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
GTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATCACATGGTCTGCTGTGCGGCGCATTTGTCGGTGAAACGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTG > CP009273/3379590‑3380111 | gTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATCACATGGTCTGCTGTGCGGCGCATTTGTCGGTGAAACGCCGAAAAATCCTCGTTAAACGGGAACGGTGCATCAAGCAGGCGACGGTGCGCGATAATCATAGTCAGcccccg > 1:200548/1‑273 (MQ=255) ttCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATCACATGGTCTGCTGTGCGGCGCATTTGTCGGTGAACCGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGGGGTTCTCTTACAGACGTg > 1:82827/1‑275 (MQ=255) tGGTAGATTTCCAGGTCTGACAGCAACTGGCCGAGGTGCCTACGACGTTTATCTGTGCTACGGGCAGGTATCACATGGGCTGCTGTGCGGCGCTTTTGTCGGTGGAACGCCGATAAATCGTCGTTAACCGGGATGGGTGCATCACGCGGGCCGGGGTGCGCGATAAGCATCGTCAGCGCCAGGCGAAATGTCTGCAAATCCCCTGGGAACTTATGCATCAGCAAAAACAGCCGCTGACAAAGTGCCGGGAGGTGGTTCTCTTAACGACGTg < 2:88507/271‑1 (MQ=255) ggTTTGTCATCGCTACTCGACGATAGCCCATGGTCTGCTGTTCGGCGCATTTGTCGGTGAAACGCCGATAAATCCTCGTTTACCGGGATCGGTGCAGCACTCCGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAg < 2:82825/274‑1 (MQ=255) tgactttccatttcagtcagtttctcgacagcgcattagggcaaatcgtcggctgtgtgctcgcgttcaccgttattttGCTGGTGCGGGATAA‑‑ATCG‑C‑GCGACAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCaaaaa < 1:171149‑M1/72‑1 (MQ=255) tgactttccatttcagtcagtgtgacgacagcgcattagggcaaatcgtcggctgtgtgctcgcgttcaccgttattttGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAggtg < 1:230869‑M1/103‑1 (MQ=255) tttccatttcagtcagtttctcgacagcgcattagggcaaatcgtcggctgtgtgctcgcgttcaccgttattttGCTGGTGCGGGATAA‑‑ATCG‑C‑GCGACAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAgc > 2:171145‑M1/76‑151 (MQ=255) tttccatttcagtcagtttctcgacagcgcattagggcaaatcgtcggctgtgtgctcgcgttcaccgttattttGCTGGTGCGCGa > 2:71862‑M1/76‑87 (MQ=255) tttccatttcagtcagtttctcgacagcgcattagggcaaatcgtcggctgtgtgctcgcgttcaccgttattttGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTtc > 2:230862‑M1/76‑182 (MQ=255) tttccatttcagtcagtttctcgacagcgcattagggaaaatcgtcggctgtgtgctcgcgttcaccgttattttGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCGGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACACTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTg > 1:90516‑M1/76‑238 (MQ=255) gTCTGCTGTGTGGCGCATTTGTCGGTGAAACGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCTACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTCCTGTCGCGCGATTTAt > 1:327735/1‑275 (MQ=255) gTCTGCTGTGCGGCGCATTTGTCTGTGAAACGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCgg > 1:235366/1‑224 (MQ=255) gTCTGCTGTGCGGCGCATTTGTCGGTGAAACGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGAGCGATAATCATCGTCCGCGCCAGGCGAAATTTCg > 2:272873/1‑116 (MQ=255) tgctgTGCGGCGCATTTGTCGGTGAAACGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTNCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCAGGTCCTGTCGCGCGATTTATccc > 1:288677/1‑275 (MQ=255) gctgTGCGGCGCATTTGTCGGTGAAACGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAATCATCGTCAGCTCCAGGCGAAATTTCGGCaa < 1:272884/116‑1 (MQ=255) gctgTGCGGCGCATTTGTCGGTGAAACGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGa < 2:235359/224‑1 (MQ=255) cggcgCATTTGTCGGTGAAACGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGc > 1:25246/1‑185 (MQ=255) gcATTTGTCGGTGAAACGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCcaca < 2:25246/185‑1 (MQ=255) tGAAACGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAg > 2:336481/1‑119 (MQ=255) aCGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATCCATCAGCaaa < 1:336493/119‑1 (MQ=255) gATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAGTCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTCCTGTcgc < 1:175271/232‑1 (MQ=255) aGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGACATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGt > 1:111620/1‑123 (MQ=255) aGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGc > 2:284446/1‑98 (MQ=255) GCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCggg > 1:284457‑M1/1‑98 (MQ=255) GCTGGTGCGCGATAATCAGCGTCAGCGCCAGGCGAAAGTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCca > 2:111618‑M1/1‑123 (MQ=255) CTGGTGCGGGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAgg < 1:159804‑M1/100‑1 (MQ=255) TGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGc > 2:298701‑M1/1‑119 (MQ=255) GGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTCTTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTacgac < 1:191205‑M1/114‑1 (MQ=255) GGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGCCCGGTCCTGTCGTGCGATTTAACCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGACTTGCCCGAATGCGATGNTAAGaaactgacgg < 2:248077‑M1/275‑3 (MQ=255) TGCGGGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAggtggt > 2:159800‑M1/1‑100 (MQ=255) CGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGc > 2:191201‑M1/1‑114 (MQ=255) GCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTCCTGTCGCGCGATTTATc < 1:182508‑M1/194‑1 (MQ=255) | GTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCCAAAGTACCGACGACGTTTATCATCGCTACGGGCAGATATCACATGGTCTGCTGTGCGGCGCATTTGTCGGTGAAACGCCGATAAATCCTCGTTAACCGGGATCGGTGCATCACGCAGGCGCTGGTGCGCGATAATCATCGTCAGCGCCAGGCGAAATTTCGGCAAATCCCCTGGGAACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTG > CP009273/3379590‑3380111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |