| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 370,475 | Δ1 bp | 16.7% | intergenic (+138/‑440) | mhpE → / → mhpT | 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 370,475 | 0 | C | . | 16.7% | 69.0 / 9.9 | 24 | intergenic (+138/‑440) | mhpE/mhpT | 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter |
| Reads supporting (aligned to +/- strand): ref base C (12/8); new base . (2/2); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01 | |||||||||||
GTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTG > CP009273/370242‑370740 | gTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATCCGGCGTGAACGCCTTATCCgg > 1:358754/1‑274 (MQ=255) tatTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGAGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc > 1:631193/1‑274 (MQ=255) gCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCATACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTACCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAAttt > 1:689595/1‑274 (MQ=255) gCCGGATGGTTGGCGGCCAGGATGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGAGGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGc < 1:447596/274‑1 (MQ=255) ttGGCGGCCAGGAGGATATGAGTGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGa < 1:239238/274‑1 (MQ=255) ttGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGTACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCt > 1:376708/1‑275 (MQ=255) ttGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGgcg > 2:519795/1‑226 (MQ=255) ttGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGGCGCATCAGGCATCt > 1:181685/1‑275 (MQ=255) gACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGCAAAGCGTCGCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGC‑CCATAAACCGTAGGCCTGATAAGACGCGCATAGCGTCGCATCAGGCAt < 1:209336/271‑1 (MQ=17) cGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCggg < 1:519802/226‑1 (MQ=255) gcgcTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAATACGCG‑TTAGCGTNGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGa > 1:613325/1‑275 (MQ=255) acaacaaATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAATGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATgcggcg > 1:362356/1‑274 (MQ=255) gatgaCTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCAT‑GGCGCACGACTGcc > 1:6254/1‑150 (MQ=255) aCTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCAT‑GGCGCACGACTGCCGGAt < 2:6254/150‑1 (MQ=255) tggggaTGCCCCTCCCCGCACGACGGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGGGAGAATTTGTAGGTCTGATGAGACGCG‑TTAGCGTCGCAGCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCgaga < 2:181682/269‑1 (MQ=255) tCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGgcgc > 2:107596/1‑157 (MQ=255) aGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGa < 1:107597/157‑1 (MQ=39) aCGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAACCGCCTTATCCGGCCAACGGa > 1:115332/1‑275 (MQ=255) tCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAAt > 2:613084/1‑175 (MQ=39) cGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGAt > 2:94958/1‑62 (MQ=33) gCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACACTTCGC‑CCATAAACCGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTg > 1:137749/1‑275 (MQ=14) aTCAGGCATCTGCGCACGACTGCCGGATGCGGCNTGAACGACTTATACGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATaa < 1:607649/186‑1 (MQ=255) aTCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGt < 1:613093/175‑1 (MQ=39) tCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGgcg < 1:94959/62‑1 (MQ=35) | GTGGATATTCTCGTTGAGCTGGGCAAACGCCGGATGGTTGGCGGCCAGGAGGATATGATCGTTGACGTGGCGCTGGATCTGCGCAACAACAAATAATGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTG > CP009273/370242‑370740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |