| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,657,965 | G→A | 12.9% | A34T (GCC→ACC) | ynfH → | oxidoreductase, membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,657,965 | 0 | G | A | 12.9% | 80.2 / 5.4 | 31 | A34T (GCC→ACC) | ynfH | oxidoreductase, membrane subunit |
| Reads supporting (aligned to +/- strand): ref base G (15/12); new base A (2/2); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01 | |||||||||||
GAAGAGCTGCGCCAGAAGCACGGCACTCTTGCTGCCGTCGCGCCACTGCCGCGCGCGCATTTCACAAAACCCAATA‑TCGTTATCAAACCTAACGCCAACAGCCGCCCGACCGGCGATACCACTGGTTATCTGGCTAATCCGGAGGAAGTGTAAAATGGGAAATGGATGGCATGAGTGGCCACTGGTTATCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTGGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGT > CP009273/1657712‑1658217 | gggcagcggncccAGAAGCACGGCACCCTTGCTGCCGTCGCGCCACTGCCGCGCGCGCATTTCACAAAACCCAATA‑TCGTTATCAAACCTAACGCCAACAGCCGCCCGACCGGCGATACCACTGGTTATCTGGCTAATCCGGAGGAAGTGTAAAATGGGAAATGGATGGCATGAGTGGCCACTGGTTATCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTc < 2:275153/264‑1 (MQ=255) tgcCCTCACCCCACCGCCGCGCCGGCACTGGCCAAAACCCAATACTCG‑TATCAAACCTAACCCCAACAGCCGCCCGACTGGCGATACCACTGGTTATCGGGCTAATCCGGAGGAAGTGTAAAATGGGAAATGGATGGCATGAGTGGCCACTGGTTATCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGTCGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTgg < 2:190110/275‑1 (MQ=255) cgcgcgcgCATTTCACAAAACCCAATA‑TCGTTATCAAACCTAACGCCAACAGCCGCCCGACCGGCGATACCACTGGTTCTCTGGCTAATCCGGAGGAAGTGTAAAATGGGAAATGGATGGCATGAGTGGCCACTGGTTATCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCg < 1:719217/274‑1 (MQ=255) cgaacacacctCGTTATAAAACCCAACGCCCACAGCCGGCCGACAGGCGATGCCACTGGTTATTGGGCTAAGCCGGAGGAAGTGTAAAATGGGAAATGGATGGCATGAGTGGCCACTGGTTATCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCa < 2:650845/265‑1 (MQ=255) aCCTAACGCCAACAGCCGCCCGACCGGCGATACCACCGGTTATCTGGCTAATCCGGAGGAAGTGTAAAATGGGAAATGGATGGCATGAGTGGCCACTGGTTATCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGTCGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACt > 1:399994/1‑274 (MQ=255) ccGACCGGCGATACCACTGGTTATCTGGCTAATCCGGAGGAAGTGTAAAATGGGAAATGGATGGCATGAGTGGCCACTGGTTATCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTg > 1:682459/1‑233 (MQ=255) ccGGCGATACCACTGGTTATCTGGCTAATCCGGAGGAAGTGTAAAATGGGAAATGGATGGCATGAGTGGCCACTGGTTATCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCAt < 2:682450/233‑1 (MQ=255) tAATCCGGAGGAAGTGTAAAATGGGAAATGGATGGCATGAGTGGCCACTGGTTATCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATTCACCTGTGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCGGGTCTGAGTCAc > 2:232380/1‑275 (MQ=255) cATGAGTGGCCACTGGTTATCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCctct > 1:602303/1‑136 (MQ=255) gCCACTGGTTATCTTTACCGTATTGGGTGAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCGGTCATGCACCTGGGATCGCCACTNCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTAt > 1:524460/1‑275 (MQ=255) gTTATCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAg > 1:472893/1‑85 (MQ=255) tCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCg < 2:472886/85‑1 (MQ=255) tACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGACACTGCGGGCGTTTAACTCCCTgaatcg > 2:387043/1‑192 (MQ=255) ccGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCCTGtttt > 2:619815/1‑105 (MQ=255) gTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTgaatcgaatc < 1:387049/192‑1 (MQ=255) aTTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCct < 1:619824/105‑1 (MQ=255) aTGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTGGCAGTCATCGGTAAAATGc > 1:79532/1‑275 (MQ=255) aTGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGCTTTTACTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGTCACTGCGGGCGGTTAACTCCCGGAATCGAATCGGTGCGGCGGGTCTGAGTAACGGGATTGCGTCCGGTTACCTATTTTTGGCCGGTGGCGGATTGTGGGTGGGGGGGGCAGTAATCGGTAAAATgg > 2:632020/1‑274 (MQ=255) tGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCg > 2:601550/1‑159 (MQ=255) tGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCACCAAAAATGACGCAg > 1:537079/1‑61 (MQ=255) tagtagGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTa < 1:601559/159‑1 (MQ=255) tagtagGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCACCAAAAATGACGCAGACCg < 2:537071/61‑1 (MQ=255) tagtagGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCACCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTg > 2:262128/1‑186 (MQ=255) agGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCACCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCt < 1:262133/186‑1 (MQ=255) cgcTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCgg > 2:281197/1‑103 (MQ=255) cTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGtttt > 1:579801/1‑76 (MQ=255) gATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGttt < 1:281202/103‑1 (MQ=255) ttGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCct < 2:579792/76‑1 (MQ=255) aGCGGAATTGCCTGGTTCGCCGCCTAAAATTACGCAACCCGTCAGCGTATTGTCCGCGTCATGTTTTTCCTCTGTCTGGTGATGGGCGTCGGGTTTATTGCCTCTGTCCTCCACCTGGGCTCGCCACTGCGGGCGTTTAACTACCTGACTCGAATCGGATCTTCTGGTCTGACTAACGATACTGCTGCCGGCTCCCTATTTTTTGCCTTTGGCGGATTCTTGTTGTGGGGGTCTGTCATACGTTCAATGCCGCCGTCGGTGGGGAAAGTCTggg > 2:209198/1‑273 (MQ=255) aTTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTAtt > 1:392824/1‑93 (MQ=255) gCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCct < 2:392818/93‑1 (MQ=255) | GAAGAGCTGCGCCAGAAGCACGGCACTCTTGCTGCCGTCGCGCCACTGCCGCGCGCGCATTTCACAAAACCCAATA‑TCGTTATCAAACCTAACGCCAACAGCCGCCCGACCGGCGATACCACTGGTTATCTGGCTAATCCGGAGGAAGTGTAAAATGGGAAATGGATGGCATGAGTGGCCACTGGTTATCTTTACCGTATTGGGTCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTGGCAGTCATCGGTAAAATGCCGCAAGCGTTGGGGAAACTCTGGT > CP009273/1657712‑1658217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |