| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,532,103 | G→C | 11.5% | C280W (TGC→TGG) | cysM ← | cysteine synthase B (O‑acetylserine sulfhydrolase B) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,532,103 | 0 | G | C | 11.5% | 98.2 / 2.5 | 34 | C280W (TGC→TGG) | cysM | cysteine synthase B (O‑acetylserine sulfhydrolase B) |
| Reads supporting (aligned to +/- strand): ref base G (15/14); new base C (2/2); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTT‑ATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCC > CP009273/2531849‑2532321 | cATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTT‑ATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGccaccacca > 1:92769/1‑274 (MQ=255) aaaaaaacaggggtGTTTAATGCCGGTGGAGGAGTAAACGCCTTATCCGGCCCACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCAc < 2:348576/262‑1 (MQ=38) gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAACGATCGCCACGATCCCAGAAGATCGCCACCACCACCCCCTCAAGGTTAACTTTTGCCACCCCCAGTGccactggccctt > 1:348582/1‑263 (MQ=37) tacAAAATCTTGCAGCAACCTTGTAGGCCTGATCAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGtt < 2:92768/273‑1 (MQ=255) tGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCGCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAg > 1:102491/1‑209 (MQ=255) aaGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCGCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGa < 2:102490/209‑1 (MQ=255) cAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGGTCGCCACGATCTCAGATTATCGCCACCACCACCGCGCCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCCCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCCCACCACCAGTTCGCGCACGGTGTTTTCCGCATCGCTCTGATTAACCTCCATCAcc > 2:326206/1‑273 (MQ=255) cAGGCAGTTTTGCGCTGGTCATCAGTCTCCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATTCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATAGCCACGATCGCAgat > 1:404571/1‑122 (MQ=255) cAGTTTTGCGTTTGTCATCAGTCTCCGATGCTATTAATCCTTAAATCACCGCCCCCTGGCTAAAATGCTCTTCCCCAAGCACCCCGGTAGAAAGGTAGCGATCGCCACGATCCCATATGATc < 2:404564/122‑1 (MQ=255) ttttGCGTTTGTCATCAGTCTCCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCaccaccacc > 2:720022/1‑131 (MQ=255) gCGTTTGTCATCAGTCTCCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGt < 1:720032/131‑1 (MQ=255) aTGCTATTAAACCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCGCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGc < 1:326212/275‑1 (MQ=255) tATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGccacc > 2:21107/1‑97 (MQ=255) aaTCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGccaccacca < 1:21107/97‑1 (MQ=255) aaTCCTTAAACCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGANCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATACTc > 1:601716/1‑275 (MQ=255) tCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCGCCTCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGc < 1:446928/265‑1 (MQ=255) tAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCTAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCATGGTTAGCTTTTGCCACCCGCAGTGCTCCCGCAACCGCGCCTCCGGAGCTGACTCCACAGACTATTCCGTCCCGCACCCCCAGTTCGCGCCTGGTGTTTTCCGCATTGCCCTGACGAATACCCAGCACCTAACCCACCAGGGAAGCGTAGAAACTCACCGCCAAATATTccnagc > 2:150553/1‑270 (MQ=255) tAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACAACCCCCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGAc > 2:326483/1‑152 (MQ=255) tAAATCCCCGCCCCCTGGCGAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTACTTCCCGAACCGCCAGTTCGCGCCTGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGAAATTCCgacg > 1:585685/1‑271 (MQ=255) tCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCca < 1:326489/152‑1 (MQ=255) gggaacaaaCGCTATTCCCCACACCCCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCCCCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGcc < 2:601707/269‑1 (MQ=255) tctTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCca > 2:464025/1‑129 (MQ=255) ctTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACAGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCAGTAGGCCAgcg > 1:401496/1‑253 (MQ=255) ccccAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGa < 1:464032/129‑1 (MQ=255) cccGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGc > 2:366603/1‑162 (MQ=255) cccGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGCTcgcg > 1:574310/1‑147 (MQ=255) gTAGAAAGGTAGCGATCGCCACGTTCGCCGATGCCCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCCCCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATg < 2:574301/147‑1 (MQ=255) gTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATcg < 1:366609/162‑1 (MQ=255) gCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTc > 1:238823/1‑144 (MQ=255) gCGATCGCCACGATCCCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAAc > 2:652110/1‑78 (MQ=255) tCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCa < 2:238818/144‑1 (MQ=255) tCGCCACGATCCCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACcgcg < 1:652119/78‑1 (MQ=255) cACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCa > 2:418943/1‑51 (MQ=255) aTCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCg < 1:418950/51‑1 (MQ=255) | CATTTGTCGGCAGCAACAACTGTTAAAAAAGTGCGCTTTGTTT‑ATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTATTAATCCTTAAATCCCCGCCCCCTGGCTAAAATGCTCTTCCCCAAACACCCCGGTAGAAAGGTAGCGATCGCCACGATCGCAGATGATCGCCACCACCACCGCGTCAGGGTTAGCTTTTGCCACCCGCAGTGCTCCGGCAACCGCGCCGCCGGAGCTGACGCCACAGAATATTCCTTCCCGCACCGCCAGTTCGCGCATGGTGTTTTCCGCATCGCGCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCC > CP009273/2531849‑2532321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |