Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,043,037	C→T	8.4%	V330V (GTG→GTA)	gcvT ←	aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,043,037	0	C	T	8.4%	130.4 / 3.9	48	V330V (GTG→GTA)	gcvT	aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base C (20/24);  new base T (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

ACAAACACCATATCGCCTAACAGCTCCTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTC  >  CP009273/3042781‑3043306                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                               acaaacaCCATATCGCCTAAACGCTCCTGAGCATGTTCGGTAATACCATCGGTGTAAGTGCCGTCGGCTTCTTTACGAAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATg                                                                                                                                                                                                                                                              <  2:315458/275‑1 (MQ=255)                ccTAACAGCTCCTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGGCGGCTTTGCCGTTACGCACAAACACAGCTTTTGTCACTTTAACCAGCATTTCACGGTTGCGAATTTGCACAATCGACGTTTCGCCAATACCTTCCGACACGAGCGCCAGCACAATGCTGTCACCCCGCGt                                                                                                                                                                                                                                                >  1:348954/1‑274 (MQ=255)                  tAACAGCTCCTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTAGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCgg                                                                                                                                                                                                                                             <  1:373997/275‑1 (MQ=255)                        ctctTGATCATTTTTGGTAATACCTACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGaa                                                                                                                                                                                                                                       <  1:455612/275‑1 (MQ=255)                                  tGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGTAcgctttaccg                                                                                                                                                                                                                                                                     >  2:125843/1‑228 (MQ=255)                                      cGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGTAcgctttaccgatgc                                                                                                                                                                                                                                                                 <  1:125844/235‑12 (MQ=255)                                                 aCGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAgc                                                                                                                                                                                                                                                                    >  1:339203/1‑221 (MQ=255)                                                   ggTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAAGGCCTTCAt                                                                                                                                                                                                             >  1:307127/1‑274 (MQ=255)                                                   ggTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCCGGTTAtcctgcgtaacat                                                                                                                                                                                                            <  1:311302/275‑13 (MQ=255)                                                   ggTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGGTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAgc                                                                                                                                                                                                                                                                    >  1:113451/1‑219 (MQ=255)                                                     tgtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCaa                                                                                                                                                                                                                                                                <  2:339197/221‑1 (MQ=255)                                                       tAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGTACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTgg                                                                                                                                                                                                        <  2:235209/275‑1 (MQ=255)                                                       tAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCAGCATTTCACGGTTGCGAATTTGCACAATCTCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGACAAAGTACCGCTGGGGATAATGCCTTCCTgag                                                                                                                                                                                                          >  2:195986/1‑271 (MQ=255)                                                             ccGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGTAcgctttaccg                                                                                                                                                                                                                                                                     >  2:29957/1‑201 (MQ=255)                                                               gTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTg                                                                                                                                                                                                                          >  2:638980/1‑249 (MQ=255)                                                                   gCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATaa                                                                                                                                                                                                                      <  1:638989/249‑1 (MQ=255)                                                                                 ccATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAAACAATCTCCAAAAAAGTAAATCATGCGACGGCTTTGCCGTTACGCACAAAAACAGGGTTTATCACTTTAACAGGCATTTCACGGTTGCGAATTTACACAATCGCAATTTCGCCAATACCTTCCGGCACGCCCGCCAGCGCCATGCTGTAACCCAGCGTCGGGGAGAAAGTACAGCTGGAGCTAAAGACTTCAGGCAGGATGCACTGCGAC‑TCgggatacaagac                                                                                                                                                                             >  2:220706/1‑265 (MQ=255)                                                                                     tCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCAcg                                                                                                                                                                                                                                                                             <  1:382288/176‑1 (MQ=255)                                                                                     tCGTGNTCTTTGCTGTATCTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAATCAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATTCCTTCCGGCACGCGCGCCAGCGCaa                                                                                                                                                                                                                                                                <  1:461022/189‑1 (MQ=255)                                                                                              ttGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCAAGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTGTGTGACTTTAACCGGCACTTCACGGTTGCGAATTTGACCAATCACCGTTTCGCCAATCCCTTACGCCACGCGCGCCAgc                                                                                                                                                                                                                                                                    >  2:374498/1‑176 (MQ=255)                                                                                               tGCTGTATTTCAGTTCTGCTGGTACGTTGCACATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGACAATACCTTCCGGCACGCACGCCAGCGCAATGCTGTAACCCACCGTCCGGGAGAACGTACCGTGGGTGATAATGCCTTCATGCTGGTTGCCGTG‑GCATTGGGTAAAGCGTAGCTGCCGGTCCTTAc                                                                                                                                                               >  2:636827/1‑275 (MQ=255)                                                                                                  tGTATTTCAGTTCTGCTGGGACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGTCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCACTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCaa                                                                                                                                                                                                                                                                <  1:374504/176‑1 (MQ=255)                                                                                                     ttttCAGTCCTTCTGTTCCGTTGCTCCTCAAACAAGCTACCTAAAAGTACATCAAGCGGCGGCTTTTCCGTTACGCACAAAAACAGGTTTTGTCACGTTAACCTGCATTTCACGGTTGCTAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTAAAGCGTACCGGCAGATcgtgactttatc                                                                                                                                                         <  2:719794/274‑12 (MQ=255)                                                                                                      tttCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGAAACCCAGCGTCg                                                                                                                                                                                                                                              >  1:400383/1‑190 (MQ=255)                                                                                                          aGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGga                                                                                                                                                                                                                                          <  2:400376/190‑1 (MQ=255)                                                                                                            ttCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGcc                                                                                                                                                                                                                                                                       >  1:37550/1‑159 (MQ=255)                                                                                                              ctgctgGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCAcgc                                                                                                                                                                                                                                                                            >  2:501449/1‑152 (MQ=255)                                                                                                                gctgGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAgcg                                                                                                                                                                                                                                                                   <  2:37550/159‑1 (MQ=255)                                                                                                                  tgGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCGCTTTCTCCAATACATTCCGGCACgcgcgc                                                                                                                                                                                                                                                                        <  1:501456/152‑1 (MQ=255)                                                                                                                          tGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAgc                                                                                                                                                                                                                                                                    >  2:475611/1‑148 (MQ=255)                                                                                                                              catcaATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCaa                                                                                                                                                                                                                                                                <  1:475618/148‑1 (MQ=255)                                                                                                                                    tcaatcTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCAGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTCGGTCATCACCAGACCAACCAg                                                                                                                          <  1:195989/275‑1 (MQ=255)                                                                                                                                                           cgcgACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCgggg                                                                                                                                                                                                                                           >  1:654178/1‑140 (MQ=255)                                                                                                                                                               aCGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGaa                                                                                                                                                                                                                                       <  2:654169/140‑1 (MQ=255)                                                                                                                                                                                               tGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGNCAGCGCAATGCTGTAACCCTGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACg                                                                                <  1:555051/258‑1 (MQ=255)                                                                                                                                                                                                                        ttGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCg                                                                                                                                                                                                                                                 >  1:156203/1‑73 (MQ=255)                                                                                                                                                                                                                           cGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGcc                                   >  1:39989/1‑275 (MQ=255)                                                                                                                                                                                                                            gAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCgg                                                                                                                                                                                                                                             <  2:156200/73‑1 (MQ=255)                                                                                                                                                                                                                                  gCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAAccc                                                                                                                                                                                                                                                     >  2:294477/1‑59 (MQ=255)                                                                                                                                                                                                                                  gCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTAAAGCGTACCGGCAGTTGATTACGCAGCACGCCTTTTTCGGTCATCACCAGACCAAACAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTAACGATCTGCAGGTTCCCAGGCGATGGTCCAGCCCATGTTg                            >  1:47997/1‑275 (MQ=255)                                                                                                                                                                                                                                      aaTCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCg                                                                                                                                                                                                                                                 <  1:294483/59‑1 (MQ=255)                                                                                                                                                                                                                                        tCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCttttt                                                                                                                                                >  1:424046/1‑153 (MQ=255)                                                                                                                                                                                                                                          gCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTAAAGCGTAGCGGCAATTCATTACGCAGCACGCCTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTa                     <  1:636836/274‑1 (MQ=255)                                                                                                                                                                                                                                            cGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTCGGt                                                                                                                                            <  2:424039/153‑1 (MQ=255)                                                                                                                                                                                                                                                  gCCATTTCCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGgagaga            <  1:279667/275‑1 (MQ=255)                                                                                                                                                                                                                                                      aTACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTaaa                                                                                                                                                                                   >  1:131439/1‑104 (MQ=255)                                                                                                                                                                                                                                                          cTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTAAAGCGt                                                                                                                                                                               <  2:131438/104‑1 (MQ=255)                                                                                                                                                                                                                                                            tCCGGCACGCGGAGCAGCGCAATGCAGTACCCCTGCGTCGGGGGGAAAGTACCGCTGTTGATAATGCCTTCATGCTGGTGGCCCTGCGCA‑TCGGTAAAGCGGACCGGCAGTTCATTACGCAGCACGCCTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTc  <  2:39989/275‑1 (MQ=255)                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                               ACAAACACCATATCGCCTAACAGCTCCTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGCATTTCACGGTTGCGAATTTGCACAATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCA‑TCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCGTC  >  CP009273/3042781‑3043306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑