| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,123,519 | A→C | 8.1% | intergenic (‑17/‑18) | yghO ← / → insH1 | pseudogene, DNA‑binding transcriptional regulator homology/IS5 transposase and trans‑activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,123,519 | 0 | A | C | 8.1% | 118.4 / 2.7 | 41 | intergenic (‑17/‑18) | yghO/insH1 | pseudogene, DNA‑binding transcriptional regulator homology/IS5 transposase and trans‑activator |
| Reads supporting (aligned to +/- strand): ref base A (19/18); new base C (2/2); total (21/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCAAGGTATCTATTTGCGCGGTAATACGCCCAACTATCTGCCCCGCTTTTTTTGCCACCCACGCCTGCCAGATGATATGGTCCGTCCCCGGATTTTTCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCTGGAAACCATGCTACGCATTCA > CP009273/3123246‑3123763 | aTGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCAAGGTATCTATTTGCGCGGTAATACGCCCAACTATCTNCCCCGCTTTTTTTGCCACCCACGCCTGCCAGATGATATGGTCCGTCCCCGGATTTTTCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAg < 1:506845/275‑1 (MQ=255) ttGCGCGGTAATACGCCCAACTATCTGCCCCGCTTTTTTTGCCACCCACGCCTGCCAGATGATATGGTCCGTCCCCGGATTTNTCGCAGACAAATGCTCGTTGCGGTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTgg < 1:651475/241‑1 (MQ=255) ccAACTATCTGCCCCGCTTTTTTTGCCACCCACGCCTGCCAGATGATATGGTCCGTCCCCGGATTTTTCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTCTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGa > 1:444276/1‑226 (MQ=255) aaCTATCTGCCCCGCTTTTTTTGCCACCCACGCCTGCCAGATGATATGGTCCGTCCCCGGATTTTTCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCGTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGATCATCATGAGTCATCAACTTACCTTCGCCGACAGt > 1:422933/1‑274 (MQ=255) cTATCTGCCCCGCTTTTTTTGCCACCCACGCCTGCCAGATGATATGGTCCGTCCCCGGATTTTTCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCa < 2:444269/226‑1 (MQ=255) ttGCCAAATGTTTTGGTCCGTCCCCGGATTTTTAGCATACAAATGGTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAag < 1:574924/274‑1 (MQ=255) tatGGTCCGTCCCCGGATTTTTCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTAATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAg > 1:467212/1‑148 (MQ=255) gtccgtccCCGGATTTTTCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTAATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTccc < 2:467205/148‑1 (MQ=255) cgtccCCGGATTTTTCGCAGACAAATGCTCGTTGCGTTCAATTAATAAAGGGGGTATCCAGTTTGGAGCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGNGATCATGTTTGTCATCTgta > 1:614392/1‑171 (MQ=255) tccCCGGATTTTTCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAAc > 1:717631/1‑181 (MQ=255) tccCCGGATTTTTCGCAGACAAATGCTCGTGGCGATCAATGAATAAAGGGGGTATCCAGATTGGCTCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGACTTAAGGTCATTTTTATTAAGGAAGGAGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCTTCATGAGTCATCAACTTACATTAGACGACAGTGAAATAAGCAGTAAGAGCCGACAGACAAGAAAAGAGATTTACCTGTCCCCCAt > 2:238252/1‑274 (MQ=21) tccCCGGATTTTTCGCAGACAAATGCACGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTACCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACATGGAATTCAGCAGTAAGCGGCGTCAGACCAGAAAAGCGATTGTCTTGTCCCGCag > 2:226166/1‑273 (MQ=255) cacGGATTTTTCGCAGACAAATGCTAGGTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCAGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCAAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCa < 2:614383/171‑1 (MQ=255) cGGATTTTTCGCAGACAAATTCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAg < 1:226171/274‑1 (MQ=255) cGGATTTTTCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAggg < 2:717621/181‑1 (MQ=255) gATTTTTCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGt > 1:54619/1‑157 (MQ=255) tttCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATc < 2:54619/157‑1 (MQ=255) tttCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATg > 2:679494/1‑184 (MQ=255) gCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGtc < 1:679503/184‑1 (MQ=255) gACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTTTCCCGCATGGAGCAGagacttccaat > 2:97758/1‑265 (MQ=255) gTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTAGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTg > 1:467100/1‑194 (MQ=255) cGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAAtt < 2:467093/194‑1 (MQ=255) aGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTCAGGTCATTTTTATTAAGGAAGGTGCGAACaa > 2:536237/1‑92 (MQ=255) ggTATCCATTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCACTTTTATTAAGGCAGGTGCGAACAAGTcc < 1:536245/92‑1 (MQ=255) gTATCCAGTTTGGATCGTCGGGATAACGTGATGATGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTAAATTCAGCAGTANGCGCCGTCNGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGATGGAAGTCATCGAGCCGTTTTAc > 1:472138/1‑275 (MQ=255) gTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACaa > 1:624104/1‑87 (MQ=255) gTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACaa > 2:181196/1‑87 (MQ=255) gTATCCAGTTTGGATAGCCGGGATACAGTGATGACGGAAAAGCAATAAATGCCTTAAGGTCATCTTTATTAAGAAAAGCGCGAACAAGTCCCTGATATCAGATAATGTTTGTCATCTGGAGCAATAGCACAGGGTTCATCATGAGTCATCAACTTACATCCGCTCACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAAATTAACATATCCCGCATGGAGCAGATACTGCCATGGCAAAACATTGTGGAAGTCACCAAGCAGTTTTa > 2:641503/1‑274 (MQ=17) ccAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTcc < 2:624095/87‑1 (MQ=255) ccAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTcc < 1:181199/87‑1 (MQ=255) gtcgGGATAAAGTGATGACGGAAAAGCGATAAATGCCCTAAGGTCATTTTTATTAAGGAAGGTGCGAACACGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAAAAGGATTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCATGAAGCGCCGTCAGACCAGAAACGAGATTTTCTTGTTCCTCATGGAGCATATTCAGCCATGGCAAAACCCGGTGGAAATCTTCGAGCCGTTTTACTCCAAGGCTGCTAATg > 2:308490/1‑275 (MQ=17) tcgGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTACCATGGCAAAACATGGTTGAAGTCATCGAGCCATTTTACCCCACGTCTGGTAAtt > 1:186517/1‑273 (MQ=255) aTAAAGTTATTACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGAGCATGTTTTTCATCTGTAGCCATAGAACCGGGTGCATCATGAGTCATCAACTTACCTCCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCgg < 1:238257/274‑1 (MQ=255) aGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTTAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGCCAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGCTTCTGCAAt > 1:356784/1‑214 (MQ=255) tgaCGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATg > 2:285524/1‑68 (MQ=255) tgaCGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCCAGGCTGGTAATGACCTGCGACCTTAt > 2:624128/1‑274 (MQ=255) tgaCGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGACGGAGACCTTAt > 1:186751/1‑274 (MQ=255) ggAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGAt < 1:285529/68‑1 (MQ=255) gAAAAGCGATAAATGACTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATAAACTTACCTTCGCCGAAAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATGTTCTTGTACAGCATGGAGCAGATTCTGACATGGCAAAACATGGAGGAAGTCATAGAGCAGTTTTACCAAAAGGCTGGAAAAGGCAGGCGaaaggaaacgcgg > 1:134063/1‑263 (MQ=14) aTGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTCAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCTGGAAACCATGCTa < 1:624137/275‑1 (MQ=38) ccTTAAGGTCATTTTTATTAAGGAAGGTGCGAACaa > 2:433506/1‑36 (MQ=255) aaGGTCATTTTTATTAAGGAAGGTGCGAACAAGTcc < 1:433513/36‑1 (MQ=255) atctCATTTTTAGTAAGGAAGGTGCGAACACTGCCCTTATATGAGCTCATGTTTGTCATCTTTACCCATAGATCAGGTTTCATCATTAGTCATCACATTACCTTCGCCGACATTGAATTCAGCAGTAAGCGCCGCCAGACCAGAAAAGTGATTTTCTTGTTCCGCATGGAGCAGAGTGTGACATGGCAAAACACGGTGGAAGTCATCGAGACGTTTTACCCCCAGGCTGGTAATGGCCGGCGACCTCATCCTCTGGAAACCATGATACGCATTCa < 2:186514/272‑1 (MQ=11) aTTTTTATTAAGGAAGGTGCGAACACGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAg > 1:148079/1‑108 (MQ=25) ttattaAGGAAGGTGCGAACACGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGaa < 2:148078/108‑1 (MQ=14) | ATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCAAGGTATCTATTTGCGCGGTAATACGCCCAACTATCTGCCCCGCTTTTTTTGCCACCCACGCCTGCCAGATGATATGGTCCGTCCCCGGATTTTTCGCAGACAAATGCTCGTTGCGTTCAATGAATAAAGGGGGTATCCAGTTTGGATCGTCGGGATAAAGTGATGACGGAAAAGCGATAAATGCCTTAAGGTCATTTTTATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCTGGAAACCATGCTACGCATTCA > CP009273/3123246‑3123763 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |