Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,528,794	A→C	100%	V145G (GTA→GGA)	envZ ←	sensory histidine kinase in two‑component regulatory system with OmpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,528,794	0	A	C	100.0%	150.6 / NA	44	V145G (GTA→GGA)	envZ	sensory histidine kinase in two‑component regulatory system with OmpR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/22);  total (22/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGC‑CTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCC  >  CP009273/3528522‑3529033                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 gcctGCCGTCCTCCCCCAGTTGCTTAACCCCACCCGCCATATGGTTAAAGGCACGGTTAACGGAACGCACCTCCGAAGCGCCNTACTCACGNAGCGGCGGCGGATTAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATTGCCCTGATGAATTTCGGTCAGCGGCACGCGTccc                                                                                                                                                                                                                                                <  1:463994/272‑1 (MQ=255)                 cAGTTGCTTAACCCCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCGGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGccgga                                                                                                                                                                                                                                <  1:230899/275‑5 (MQ=255)                                                                   gCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACgcgc                                                                                                                                                                              >  1:44058/1‑275 (MQ=255)                                                                                    tACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCNCGCGTCCCCAGATATTGGGCGACAGCCAGGCTTTCAGCCAGACGACAGGCGAAATTTTGTTGACCACAACGCGCACCTCCGTCGGgccgcc                                                                                                                                                             >  1:512542/1‑275 (MQ=255)                                                                                                        aataatCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGAt                                                                                                                                         <  1:703739/275‑1 (MQ=255)                                                                                                               ccTTTAACAACCTGGGAGGCTGCGTGTTCGAGAACGACAAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATAGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGGGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTa                                                                                                                                   <  1:587580/274‑1 (MQ=255)                                                                                                                cTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTTGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAANAGCGGAGAGAACTCGCCATGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTaa                                                                                                                                  <  1:659088/274‑1 (MQ=255)                                                                                                                        aCCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCAt                                                                                                                          >  1:344270/1‑274 (MQ=255)                                                                                                                         ccTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGAc                                                                                                                                                                                                                                 >  1:542723/1‑170 (MQ=255)                                                                                                                             cAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGcc                                                                                                                                                                                                                             <  2:542714/170‑1 (MQ=255)                                                                                                                                      gtgtTCGACCTAGTCCAACGGTCGGTTCTGGAGACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAAc                                                                                                           <  2:44058/275‑1 (MQ=255)                                                                                                                                                   aCCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGACAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTCTTGTTGACCCCAACGCGCACTTCCGCCGGgcc                                                                                                                                                                >  2:575257/1‑209 (MQ=255)                                                                                                                                                       aCGGTCGGTTCTGGATACGAATAAACAGCCACGCCACGCCTATCGCCAATAGCATAAGAGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGccc                                                                                                                                                            <  1:575266/209‑1 (MQ=255)                                                                                                                                                         gtgcggaTCTGGATACCAATAAACAGCCTCGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGCACATCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACTCGTCCCCAGATATTGGCCGACAGCCAGGGTTTCAGCCAGACCACAGGGCAACTTTTGTTGACCTCANCGCACCCTTCCGTAGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTAATAGTGTTTCGACCAACGCAGCACAGCATCTTCCGc                                                                                        >  1:519005/4‑275 (MQ=255)                                                                                                                                                                   gaaaaaaaaaaaaGAGCCACGCCCCGGATAGAGAAAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGTAGC‑CTCGTTg                                                                             <  2:344264/265‑1 (MQ=255)                                                                                                                                                                         gAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACCGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGNGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGC‑CTCGTTGGAGTa                                                                        >  1:558065/1‑274 (MQ=255)                                                                                                                                                                         gAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgctg                                                                                                                                                    >  2:260641/1‑199 (MQ=255)                                                                                                                                                                         gAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCAAGTTTGCGCGCCATCTGATGGCTTAAGGATTCAAAGTGTTGGCCCCAACGGCGACCCGCCCTCTCGG‑AGCGCACGTTGGAGTat                                                                       >  1:208401/1‑274 (MQ=255)                                                                                                                                                                             aaaCAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGcc                                                                                                                                                <  1:260646/199‑1 (MQ=255)                                                                                                                                                                                   ccACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGCATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGC‑CTCGTTGGAGTAGAGAGAGATcc                                                             >  1:41468/1‑275 (MQ=255)                                                                                                                                                                                             ccTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGt                                                                                                                                                                      >  1:66926/1‑161 (MQ=255)                                                                                                                                                                                                 tCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCggg                                                                                                                                                                  <  2:66925/161‑1 (MQ=255)                                                                                                                                                                                                        tAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACATGCGAACTTTTGTTGACCTCCACGCTCCCTTGCGTCGGGCCGCNCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGTCAGC‑CTCGTTGGAGTCGAGAGAGATCCCCAGCTCACGGTAGATCTccc                                        >  1:660752/1‑275 (MQ=255)                                                                                                                                                                                                        tAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgc                                                                                                                                                      >  1:126287/1‑166 (MQ=255)                                                                                                                                                                                                            aTAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTgcg                                                                                                                                                  <  2:126286/166‑1 (MQ=255)                                                                                                                                                                                                                     aGCGTATAGCGGAACAGCGGAGAGAAATGGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGtt                                                                                                                                                        >  1:545570/1‑151 (MQ=255)                                                                                                                                                                                                                     aGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGC‑CTc                                                                                 >  1:161104/1‑221 (MQ=255)                                                                                                                                                                                                                         tggtgCGGANCAGCGGCGAGAAATCGCCCTGGTGAATTTCGGTCCGCGGCACGAGTCCCCAGCTATTGGGCGACAGCCATGTTTTCCGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGC‑CTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGc                       <  2:274920/271‑1 (MQ=255)                                                                                                                                                                                                                         tataGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgctg                                                                                                                                                    <  2:545561/151‑1 (MQ=255)                                                                                                                                                                                                                         tataGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGC‑CTCGTTg                                                                             <  2:161101/221‑1 (MQ=255)                                                                                                                                                                                                                                 aaCAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgcc                                                                                                                                                                >  2:666334/1‑131 (MQ=255)                                                                                                                                                                                                                                 aaCAGCGGAGAGAAATCGCCCTGATGAAGTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAANGCAGACCTGCCTCTTCGGCAGC‑CTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCAcaacca                >  1:527600/1‑274 (MQ=255)                                                                                                                                                                                                                                     gCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGccc                                                                                                                                                            <  1:666343/131‑1 (MQ=255)                                                                                                                                                                                                                                      cGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATgg                                                                                                                                       >  1:45884/1‑151 (MQ=255)                                                                                                                                                                                                                                        gagagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgc                                                                                                                                                      >  1:645167/1‑134 (MQ=255)                                                                                                                                                                                                                                          gagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTa                                                                                                                                   <  2:45884/151‑1 (MQ=255)                                                                                                                                                                                                                                             aaaTCGCCCTGATGAATTTCTGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCAGCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGC‑CTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCACCTGCGTGCCg     <  1:698444/273‑1 (MQ=255)                                                                                                                                                                                                                                               aTCGACCTGAGGANTTTCCGGCAGACGCACGCGGCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCGCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGC‑CTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGtcc  <  2:527592/274‑1 (MQ=255)                                                                                                                                                                                                                                                       gatgaATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACNGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGC‑CTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTa                                                <  1:607082/220‑1 (MQ=255)                                                                                                                                                                                                                                                        atgaATTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGAt                                                                                                                                         >  1:442313/1‑131 (MQ=255)                                                                                                                                                                                                                                                            aTTTCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCt                                                                                                                                     <  2:442306/131‑1 (MQ=255)                                                                                                                                                                                                                                                             tttCGGTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTgcgc                                                                                                                >  1:343671/1‑151 (MQ=255)                                                                                                                                                                                                                                                                 ggTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCaa                                                                                                            <  2:343665/151‑1 (MQ=255)                                                                                                                                                                                                                                                                  gTCAGCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTttgtt                                                                                                                                                                                           >  2:432754/1‑71 (MQ=255)                                                                                                                                                                                                                                                                      gCGGCACGCGTCCCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGAcc                                                                                                                                                                                       <  1:432761/71‑1 (MQ=255)                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 GCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGC‑CTCGTTGGAGTAGAGAGAGATCCCCAGCTCACGGTAGATCTCCCGACGGAAAGCGGGAGGCACAACCAACTGCGTGCCGTCC  >  CP009273/3528522‑3529033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑