Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,368,798	C→A	100%	intergenic (‑37/+26)	ampC ← / ← frdD	penicillin‑binding protein; beta‑lactamase, intrinsically weak/fumarate reductase (anaerobic), membrane anchor subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,368,798	0	C	A	100.0%	199.5 / NA	61	intergenic (‑37/+26)	ampC/frdD	penicillin‑binding protein; beta‑lactamase, intrinsically weak/fumarate reductase (anaerobic), membrane anchor subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/30);  total (31/30)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAAC  >  CP009273/4368528‑4369055                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                  tAAATCAAACAACGTTTGCGGTGTGAAGTGCTGCTTTTTGGCGATTTCCGCATAGCCCCAGGAAAAGTAATAAGGTTTACCCTGATATATTACCGCCGCCGCCATACCCGGGATCTTTTGGTGCTCTATAAGCGGGGTAATTGTGCGATGCATAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGc                                                                                                                                                                                                                                                               <  1:371446/275‑1 (MQ=255)                 tgcggtttgtagggTTGCTTTGTGGGTATGTACGCATAGCCCTAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCg                                                                                                                                                                                                                                               <  1:319609/264‑1 (MQ=255)                                                                  aGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGTGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAACACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGAANTGTTAGAGTGTAAAGACAACAATCAGCTTTACAAccttcactatta                                                                                                                                                                                              >  2:439154/1‑264 (MQ=255)                                                                        aaGGTTTACCCGGTAAAATTACCGCCACCGCCATACCCGGGATTTTTTGTTGGTCTATAAGCGGGGTAATTGTGCGATGAACAATATCGTGGATTTGTGGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCa                                                                                                                                                                                        <  2:612203/275‑1 (MQ=255)                                                                              tACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGAt                                                                                                                                                                                                                                             >  1:59081/1‑216 (MQ=255)                                                                              tACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATg                                                                                                                                                                                                                                            >  2:466532/1‑217 (MQ=255)                                                                              tACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTCGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCNCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCg                                                                                                                                                                                   >  1:522593/1‑274 (MQ=255)                                                                                  cTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGt                                                                                                                                                                                                                                         <  2:59080/216‑1 (MQ=255)                                                                                  cTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGACATAGGAGGGCCGGATTTACATTGGCGATGCGtt                                                                                                                                                                                                                                        <  1:466539/217‑1 (MQ=255)                                                                                                                  tCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGTACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACaa                                                                                                                                                                                                          <  1:378522/215‑1 (MQ=255)                                                                                                                    ttttGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGc                                                                                                                                                                                                                                                               <  2:466027/160‑1 (MQ=255)                                                                                                                      ttgttgCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATGGTAACGACACCAAACAGCGTGACAACTGGCAGGATAGCAGCCAGCCCGTAGAAAACCCAATTGCCCGCAGCAACTTTGATTTTCATATc                                                                                                                                          >  2:597300/1‑275 (MQ=255)                                                                                                                                 taAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCgt                                                                                                                               <  1:580046/275‑1 (MQ=255)                                                                                                                                          gaaTTGTGGGGTANACTATTTTGTTTTTTTGTGGAGGGGCAGCAAATGGGGAGCAAGGGGATGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACCTA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATa                                                                                                                       <  2:522586/273‑1 (MQ=255)                                                                                                                                                       aattaccccgcttatagagcaacaaaagatcccgggtatggcggtggcggtaattAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGc                                                                                                                                                                                                                                                               >  1:50924‑M2/56‑125 (MQ=255)                                                                                                                                                          aaTATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAg                                                                                                                                                                                >  2:311314/1‑201 (MQ=255)                                                                                                                                                           accccgcttatagagcaacaaaagatcccgggtatggcggtggcggtaattAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGa                                                                                                                                                                                                                                                           <  2:50924‑M2/74‑1 (MQ=255)                                                                                                                                                              tCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGaaaa                                                                                                                                                                            <  1:311320/201‑1 (MQ=255)                                                                                                                                                               cGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGTATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTAAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACAACAGCg                                                                                                 >  1:397839/1‑275 (MQ=255)                                                                                                                                                                 ttGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTGACGACTCCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATc                                                                                                                                   >  1:627113/1‑240 (MQ=255)                                                                                                                                                                 ttGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACaa                                                                                                                                                                                                          >  2:628356/1‑168 (MQ=255)                                                                                                                                                                    aTTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACAtt                                                                                                                                                                                                                                                   >  1:276849/1‑124 (MQ=255)                                                                                                                                                                     tttGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGt                                                                                                                                                                                                      <  1:628365/168‑1 (MQ=255)                                                                                                                                                                       gagcaacaaaagatcccgggtatggcggtggcggtaattAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGc                                                                                                                                                                                                                                                               <  2:107835‑M2/70‑1 (MQ=255)                                                                                                                                                                        gttgAGGGGCAGGAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAAGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGa                                                                                        <  1:439161/275‑1 (MQ=255)                                                                                                                                                                        gttgAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCg                                                                                                                                                                                                                                               <  2:276844/124‑1 (MQ=255)                                                                                                                                                                          ggAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGTAGAGCGTCGTTTTGAACATA‑GGGGCTGGTTTCCAGATAAAACGGCCCGCCAGAGGAGGGCGGGATTTACATTGGCGATGCGTTAGATTTTACCGACACCAATCAGCGTGACAACTGt                                                                                                                                                                                                      <  1:357319/162‑1 (MQ=255)                                                                                                                                                                                            gAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATANCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGAAATAca                                                                    >  1:602701/1‑274 (MQ=255)                                                                                                                                                                                              gCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCCGATCGTGCATCGCGTGGTGCATACGGTGGAAACCACACCACAGAGGCAGAACCACCAGCAGNGAAAGGAATagg                                                                    >  2:524367/1‑271 (MQ=255)                                                                                                                                                                                              gCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGAACATCAGGAACAGGAATAc                                                                     >  1:67788/1‑272 (MQ=255)                                                                                                                                                                                                 agagGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCg                                                                                                                                         >  1:258306/1‑201 (MQ=255)                                                                                                                                                                                                  gagGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATAATCAGGAACAGGAATACGAGACCa                                                              >  1:139141/1‑275 (MQ=255)                                                                                                                                                                                                     gcggtaattAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCa                                                                                                                                     <  2:258301‑M2/192‑1 (MQ=255)                                                                                                                                                                                                                     cgcAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCa                                                                                                                                                                                                    >  1:630340/1‑122 (MQ=255)                                                                                                                                                                                                                     cgcAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGCCCGTAGAAAACCCATTTGCCCGCCGGTACGTGGATTTTCAGAACGTGCATCGCgtggtg                                                                                                                           >  1:400649/1‑195 (MQ=255)                                                                                                                                                                                                                        agagCGTCGTTTTGAACATA‑GGGTCTGGATTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCg                                                                                                                                         >  1:502190/1‑178 (MQ=255)                                                                                                                                                                                                                         gagCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGAt                                                                                                                                                                                                <  2:630331/122‑1 (MQ=255)                                                                                                                                                                                                                            cgtcgtTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACt                                                                                                                                                                                                        >  1:7449/1‑111 (MQ=255)                                                                                                                                                                                                                            cgtcgtTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCa                                                                                                                                     <  2:502183/178‑1 (MQ=255)                                                                                                                                                                                                                             tgcgtTTGGCANATA‑GGGGCTGGTTTCCATACAAACCGGCCCGCCATAGGAGGGCTGTATTTACATTGGCGATGGGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAAcg                                   <  2:67787/273‑1 (MQ=255)                                                                                                                                                                                                                                gtTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCa                                                                                                                                                                                                    <  2:7449/111‑1 (MQ=255)                                                                                                                                                                                                                                      aaCATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTATGCGCGAACGCCAGAACGCGCCCGTa                           >  1:237269/1‑274 (MQ=255)                                                                                                                                                                                                                                           a‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGAtt                                                                                                                                                                                                                                   >  2:578767/1‑69 (MQ=255)                                                                                                                                                                                                                                           a‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATagcagc                                                                                                                                                                                          >  2:238737/1‑110 (MQ=255)                                                                                                                                                                                                                                           a‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGcc                                                                                                                                                                                         >  1:368074/1‑111 (MQ=255)                                                                                                                                                                                                                                           a‑GGGTCTGGATTCCATACAAAACGGCCCGCAATAGGAGGGCCGGATTTACATTGGCGCTGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTAAGTGGCTTTTAAGATCGTGCATCGCGTGGTGCATACGTTGTAAACCACACCACAGCGGCAGAAAGATCATCAGGAACAGGAATACGCAACAAAAGACGCACCACGCGAACGCCGGAACGCCGTCGGAGCtaa                      >  2:303948/1‑272 (MQ=255)                                                                                                                                                                                                                                                tCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTaa                                                                                                                                                                                                                               <  1:578776/69‑1 (MQ=255)                                                                                                                                                                                                                                                tCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGa                                                                                                                                                                                      <  1:238742/110‑1 (MQ=255)                                                                                                                                                                                                                                                tCTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGAc                                                                                                                                                                                     <  2:368068/111‑1 (MQ=255)                                                                                                                                                                                                                                                 cTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCa                                                                                                                                                                                                                   >  1:509940/1‑80 (MQ=255)                                                                                                                                                                                                                                                 cTGGTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACaa                                                                                                                                                                                                          >  1:303780/1‑89 (MQ=255)                                                                                                                                                                                                                                                  aggTTTCCATAGAAAAAGGGCCGCCATAGGAGGGCCGGATTTACAATGTCGATGCGTTAGATTGTAACGACACCAATCAACGTGACAACTGTCAGGTAAGCAGCCAGGCCGTAGAAAACCCATTTGACAGCAGGTACGTGGATGTTCAGATCGTGCATCGAGTGGTGCATACGGTATAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCAt                <  2:602692/273‑1 (MQ=255)                                                                                                                                                                                                                                                   ggTTTCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATc                                                                                                                                   >  2:145526/1‑158 (MQ=255)                                                                                                                                                                                                                                                     tttCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGt                                                                                                                                                                                                               <  2:509933/80‑1 (MQ=255)                                                                                                                                                                                                                                                     tttCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGt                                                                                                                                                                                                      <  2:303774/89‑1 (MQ=255)                                                                                                                                                                                                                                                       tCCATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCgt                                                                                                                               <  1:145527/158‑1 (MQ=255)                                                                                                                                                                                                                                                         cATACAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGa                                                                                                                                                                                                                                              >  1:336673/1‑45 (MQ=255)                                                                                                                                                                                                                                                             cAAAACGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCg                                                                                                                                                                                                                                          <  2:336667/45‑1 (MQ=255)                                                                                                                                                                                                                                                               aaaCGGCCCGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGCTTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCAGCAACACGAACATCAGGAACAGGAATACGAGACCAATGAAACTCCGCGAGAACGCAAGAACGCACGAGCACCTCAGCGCATCAACNCGaacacac  >  2:271011/1‑270 (MQ=255)                                                                                                                                                                                                                                                                     cccGCCATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCa                                                                                                                         >  1:245199/1‑150 (MQ=255)                                                                                                                                                                                                                                                                         ccATAGGAGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACg                                                                                                                     <  2:245194/150‑1 (MQ=255)                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                  TAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATA‑GGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGCGAACGCCAGAACGCGCTCGTAGCTCAGCGCATCACCCGGAAACAAC  >  CP009273/4368528‑4369055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑