Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,713,241 A→G 8.1% Y207H (TAC→CAC)  anmK ← anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,713,2410AG 8.1% 136.2 / 2.1 46Y207H (TAC→CAC) anmKanhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base A (20/22);  new base G (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

ACCGCTCAACAAAACTTGTTCAGAAATGGTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCA  >  CP009273/1713002‑1713495
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                              
aCCGCTCATCAATACTTGTTCAGGAATGGTCACGTCGGGGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGcat                                                                                                                                                                                                                                <  1:380983/272‑1 (MQ=255)
  cGCTCAATAAAACTTGTTCAGAAATGGTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGcatccatc                                                                                                                                                                                                                           <  1:212696/275‑1 (MQ=255)
            aaCTTTTTCAGAAATGGTCACGGCTGTGAGTTCTGCCCGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCTGGTGCATTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTAc                                                                                                                                                                                                                 <  1:723663/275‑1 (MQ=255)
                   tCAGAAATGGTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGTTCNCTGAGCATATTTTGCAGCAGTGGGAGAATAAATTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAgg                                                                                                                                                                                                             >  1:702505/1‑272 (MQ=255)
                          gagtaacgtgcgTGAGTTCTCCCCGTGTCCACCGCACATCTCGGGCATCAACACCCGGAAAATGGCGAAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGTGCTGTTGGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGTAGTGCGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCg                                                                                                                                                                                                   <  2:702497/264‑1 (MQ=255)
                            gTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACGCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATc                                                                                                                                                                                                    >  1:768337/1‑272 (MQ=255)
                                        aGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTTCAGCCAACCATAGTTAAAGTATTCGCNTCCGGTGCTTTTCGGTTCATGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATACCTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGCCCTGACGCCAGATCCAGGCATCCATCAGCATGTTCTCAGGCCCGGTATCGTAGCCCCCAACCgg                                                                                                                                                                                     <  1:584421/275‑1 (MQ=255)
                                                    gtCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGcatc                                                                                                                                                                                                                               >  1:507871/1‑221 (MQ=255)
                                                        ccTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGcatccatc                                                                                                                                                                                                                           <  2:507866/221‑1 (MQ=255)
                                                                                               aatggcgcTCCAGCCAACCGTAGGTAAAGGTTTCCCCTCCGGTGCTTTTGGGTGACGGTGGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACGCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGGAGCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCAtt                                                                                                                              <  2:810324/275‑1 (MQ=255)
                                                                                                                     gTTAAAGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTGAGGTTTACCGGCCTGACGCCAGATCCAGGcatc                                                                                                                                                                                                                               >  2:93910/1‑156 (MQ=255)
                                                                                                                        aaaGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGTGGAGCcagca                                                                                                     >  1:286712/1‑275 (MQ=255)
                                                                                                                         aaGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTGAGGTTTACCGGCCTGACGCCAGATCCAGGcatccatc                                                                                                                                                                                                                           <  1:93910/156‑1 (MQ=255)
                                                                                                                          aGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTACCCCGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTTAGCCatcagc                                                                                                   >  1:786742/1‑275 (MQ=255)
                                                                                                                             gtgcgagTCCGGTGTTTTTCGGGTAGGGTTGCGAGAAATACGGGTGGCTTTGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCATGAACGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCa                                                                                                <  2:286708/269‑1 (MQ=255)
                                                                                                                              ttCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCANCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCat                                                                                               >  1:692716/1‑275 (MQ=255)
                                                                                                                                cgcgTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGt                                                                                                                                                                                                       >  1:77830/1‑169 (MQ=255)
                                                                                                                                    tCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCg                                                                                                                                                                                                   <  2:77830/169‑1 (MQ=255)
                                                                                                                                              tggggTGCAGGTTGAGATAAATACTTGTCGCTGAGCATATTTTGGAGCAGTGGGGGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTAc                                                                               <  2:692709/272‑1 (MQ=255)
                                                                                                                                                   tGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCTCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTTAGCACCATTCGTCCCTCGGTTGGGTGAGCCAGCAGCGCATGAAGGAACGCAGGTACCAGc                                                                           >  1:462648/1‑274 (MQ=255)
                                                                                                                                                      gggTGGCGAATAATACGGGTCGCTGAGCCTATTTTCCTGCAGGTGGAGAATACCTTTACCCGCCCGTGCCCACTCGGCAGCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCGTCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGCATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCGCGGTTGGGTGAGCCAGCAGCGCATGCTGGAACGCAGGTACCAGCgg                                                                         <  2:698731/272‑1 (MQ=255)
                                                                                                                                                        gTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTGAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCa                                                                                                                                                                                                               >  1:698877/1‑137 (MQ=255)
                                                                                                                                                           gCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGCGAATAACTTTACCCGCCCGTGCCCACTCGGTATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCACCAGCATGTTACCAGGACCGGTATCGTAGCCACCAACCGGCTGCCCAGGAATGAGCAGTTACCGATTGGCGATg                                                                                                                                                   >  2:350445/1‑194 (MQ=255)
                                                                                                                                                            cGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTGAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGAc                                                                                                                                                                                                           <  2:698869/137‑1 (MQ=255)
                                                                                                                                                             gagaAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTg                                                                >  1:179680/1‑275 (MQ=255)
                                                                                                                                                               gaAATACGGGTCGCTGGGCATATTTTGCAGCAGTGGGAGACTAACTCTACCCGCCCGTGCCCACTCGGCGTCTTTGTCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCAGCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAAGATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGt                                                               <  2:786733/274‑1 (MQ=255)
                                                                                                                                                               gaAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCAGCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTTCCCAGGAATGAGCAGTGACAGATTGGCGATgccgc                                                                                                                                               <  1:350449/194‑1 (MQ=255)
                                                                                                                                                                    aCGGGTCGCTGAGCATATTTTGCATCAGTGCGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATGCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGtcctcc                                                          <  1:258961/274‑1 (MQ=255)
                                                                                                                                                                         tCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGGGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAatat                                                                                                                                         >  1:780027/1‑190 (MQ=255)
                                                                                                                                                                           gCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCaa                                                                                                                                                                                         >  1:113588/1‑140 (MQ=255)
                                                                                                                                                                            cTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCa                                                                                                                                                                                                                          >  1:538542/1‑106 (MQ=255)
                                                                                                                                                                             tGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAg                                                                                                                                     <  2:780018/190‑1 (MQ=255)
                                                                                                                                                                               aGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCgg                                                                                                                                                                                     <  2:113588/140‑1 (MQ=255)
                                                                                                                                                                                gCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCAt                                                                                                                                                                                                                      <  2:538537/106‑1 (MQ=255)
                                                                                                                                                                                      tttGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGGACAGTGACAGANTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACgg                                       >  1:740086/1‑275 (MQ=255)
                                                                                                                                                                                             cagTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCGAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATg                                                                                                                                                   >  1:404962/1‑160 (MQ=255)
                                                                                                                                                                                                tGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTAACAGCTGTGCGCGTGGTCCTCCCAACGCAACATCCCGCCGTCCaaaagaa                              >  2:623072/1‑271 (MQ=255)
                                                                                                                                                                                                 gggAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCGAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATgccgc                                                                                                                                               <  2:404957/160‑1 (MQ=255)
                                                                                                                                                                                                  ggAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAAGTAACAGCGGTGCGCCTTGTCGTGCCAAGCCCATTTCGCGActcctcaaactac                             >  2:166091/1‑261 (MQ=255)
                                                                                                                                                                                                    ataaTAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGAc                         <  1:166091/273‑1 (MQ=255)
                                                                                                                                                                                                                cccgcccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGtcct                                                            >  1:277323/1‑228 (MQ=255)
                                                                                                                                                                                                                    cccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCa                                                        <  2:277319/228‑1 (MQ=255)
                                                                                                                                                                                                                    cccgTGCCCACTCGGCATCCTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTTAGCACCAttatt                                                                                                                           >  1:675329/1‑158 (MQ=255)
                                                                                                                                                                                                                           ccACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCca  <  2:179678/275‑1 (MQ=255)
                                                                                                                                                                                                                                         tATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCa                                                                                                                                                                                                                          >  1:35308/1‑45 (MQ=255)
                                                                                                                                                                                                                                             gTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCAt                                                                                                                                                                                                                      <  2:35308/45‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                              
ACCGCTCAACAAAACTTGTTCAGAAATGGTCACGGCGGTGAGTTCTGCCAGTGTCGCCTGCACATCTCGGGGATCAACACCCGGAAAATGGCGCAAATGGCGCTCCAGCCAACCATAGTTAAAGTATTCGCGTCCGGTGCTTTTCGGTGCAGGTTGCGAGAAATACGGGTCGCTGAGCATATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCA  >  CP009273/1713002‑1713495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑