| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 479,438 | C→A | 100% | G141V (GGC→GTC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 479,438 | 0 | C | A | 100.0% | 118.5 / ‑6.7 | 37 | G141V (GGC→GTC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (15/19); total (17/20) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCA > CP009273/479167‑479690 | gTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCTACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGGGTTGATAacgacg > 1:444680/1‑274 (MQ=255) atgCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGCGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACaa < 1:724147/273‑1 (MQ=255) gTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATc > 1:514655/1‑275 (MQ=255) cggcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACCTACGGCTGATGGCATCTTTCATATTCACCGCCACGTAGTCTGAGATATCCTCCTGCGTCATGGTTCCATCGGTGTTGCTAACGGCGACCACCATCAGCAAGCTGTTTgat > 2:769108/1‑274 (MQ=255) tagggacaggccgcAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGGTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCt < 1:401936/266‑1 (MQ=255) ccgccgCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGNTGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCCTCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCt > 1:689699/1‑275 (MQ=255) cTGTGCGTTTTGCGCGTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCCCGCCCGACGTACGGCTGATGGCATCTTTCATATTCTCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGaa < 1:665747/275‑1 (MQ=255) ggCGTTCTGCGCTTTGATGGCGGTAATGACATCAAACGGCGTTAGCTGGAAGTTGTTCAGCTCATTCGGGATCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACGTCACCCACGCCCGACGTAAGGCTGATGGCATCTTTCATATTCGCCGCCCCGTAGTCGGAGATATCCAACTGCGTCATGGTGCCAACGGGGTTGATAACGCAGCACACCACCAGGACGCGGCTGGATTATTTCTCAACGCTCACCCCCtgttccttcttct > 2:401931/1‑264 (MQ=255) tAGCTGGAATTTGTTCAGCTCATTAGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACgacgac < 1:723287/177‑1 (MQ=255) aatGGAATTTGTTTAGATACTTTAGGTTCATCCAGCTACGCATCGNGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCAc < 2:514650/272‑1 (MQ=255) aatgggggTTCATACAGAGACGCATCGCGTACTGTTAACCGAAAAACTGCACATCACCCACGACCGACGTAAGGCGGATGGCATCTTGCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATc < 2:689692/269‑1 (MQ=255) cgTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCAACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg < 1:650807/275‑1 (MQ=255) ccGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg < 1:400409/264‑1 (MQ=255) aacaacTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCGTCACCTGAACCTGCGCGATATCCACACCAGTACCCGa > 2:367484/1‑248 (MQ=255) acTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCACATCAGTACCAGACTCa < 1:367489/248‑1 (MQ=255) cccACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGAtt > 2:124388/1‑127 (MQ=255) cccACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCa > 2:712164/1‑199 (MQ=255) aCGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGa > 2:124952/1‑211 (MQ=255) cGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTtctc < 1:124388/127‑1 (MQ=255) cGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTACTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTg < 1:712172/199‑1 (MQ=255) ccGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATc < 1:124952/211‑1 (MQ=255) aTGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAg < 1:272643/275‑1 (MQ=255) ttCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACaa > 1:322360/1‑67 (MQ=255) ccgccACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCAt < 2:322356/67‑1 (MQ=255) tcctccTGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt > 1:223802/1‑182 (MQ=255) cctcctGCGTCATGGTGCCATCGTTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCACACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCTAGCTGCAGTTTGTTCTGCACCTGATCCTGCGCGATATCCGCATCAGTACCAGACTCAAGGCTCAGGGCGATCTGc > 1:416323/1‑194 (MQ=255) cctcctGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGATGCTGGATGATTTCTCAACGCTCACCCCTTCCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGCACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGGCACTGTTAGAGGACATGTACAGCAGGGTATCGAGACCGTGCATATTCTGTTCGATAACCttgt > 2:328100/1‑270 (MQ=255) cctcctGCGTCATGGTGCCATCGGTGTTGATAACGACGACAAACATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTAAGGGTGATCTGCACGGTACCCGTGTAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTAATATTCTGTTCGATAACCtgtg > 1:785845/1‑273 (MQ=255) cctGCGTCATGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg < 2:223798/182‑1 (MQ=255) ctGCGTCATGGTGCCATCGGTGTTGATAACGACGATAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACGTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAg < 1:784158/231‑1 (MQ=255) tGGTGCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGtt > 1:636907/1‑117 (MQ=255) ggTGACATGGGTGTTGAGAACGCCGACAACCCTCCGGATGCTGCTGGATGATTTCTCCACGCTTACCCCTAGCTGCTGAACTTCTGGCGGCAGCAACGGCATCGCCAGCTGCAGTTGGTTCTGCACCTGAACCTGCGCGATATCCGCAGCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCa < 2:785836/275‑1 (MQ=37) gCCATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGtt < 2:636900/117‑1 (MQ=255) ccATCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACgg > 1:262179/1‑95 (MQ=255) aTCGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAggg > 1:418863/1‑167 (MQ=255) cGGTGTTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATc < 2:262175/95‑1 (MQ=255) gtgtTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt < 2:418858/167‑1 (MQ=255) gtTGATAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCg > 2:369001/1‑217 (MQ=255) aTAACGACGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATAc < 1:369006/217‑1 (MQ=255) | GTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCA > CP009273/479167‑479690 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |