| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,885,926 | C→A | 12.2% | E523* (GAA→TAA) | yoaA ← | putative ATP‑dependent helicase, DinG family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,885,926 | 0 | C | A | 12.2% | 88.4 / 4.4 | 33 | E523* (GAA→TAA) | yoaA | putative ATP‑dependent helicase, DinG family |
| Reads supporting (aligned to +/- strand): ref base C (14/15); new base A (2/2); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-01 | |||||||||||
CGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAG > CP009273/1885666‑1886193 | cgttgtgcCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTACCCCCNGTTCGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGTTCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGTACGTCCACCCCTTCCCAGAAACTGCTgg < 1:550664/269‑1 (MQ=255) cATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTACACGGCAATTTGTCGATAATTACCCATGACAATGTATCGCCACGCACGTCCCCCCCTTCCCAGAAACTGCTGGTTGCCACAAGAACCGCAt > 1:348993/1‑275 (MQ=255) tCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCACGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACACTGTATCGCAACGCCCAACCACCCCTTCCCAAACACGGCTGGTTGCCAACAGACGCCCAtt > 2:735991/1‑274 (MQ=255) tcgtcgGCGTCGCGGATCAGTCGCCCTACCAACTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTAATCGAGCGGGTCGCCACCGAGCAAACGACAAGACTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGCGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCCCCCCTTCCCACACACTGCTGGTTGCCACAAGAAGCGCATTACAGGCACTGACCAAGAACTGCAACACNTACCCTTTGc > 2:68880/1‑274 (MQ=255) ggCGTCGCGAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAActg > 1:41102/1‑208 (MQ=255) tCGCGAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTgg < 2:41101/208‑1 (MQ=255) gTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGc < 1:416096/246‑1 (MQ=255) gCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACCGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCAACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCaac > 1:470220/1‑160 (MQ=255) cGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGNTAATTACCNATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGTAACTGCTCGGCCAGATCGCGCATCATGGCGTGCg < 1:487354/275‑1 (MQ=255) cGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCg < 1:736005/275‑1 (MQ=255) cgcAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTACCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTcccc > 2:640421/1‑194 (MQ=255) aaCGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCTGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTACCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGc < 1:640435/194‑1 (MQ=255) cAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAgg < 1:350953/200‑1 (MQ=255) tCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACACGAAGCGCATTAcc > 2:238193/1‑134 (MQ=255) tGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGgcg < 1:238196/134‑1 (MQ=255) ggATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGt > 1:564589/1‑92 (MQ=255) ggATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGAc > 2:257387/1‑122 (MQ=255) catcCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGcc < 2:564577/92‑1 (MQ=255) catcCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAAt < 1:257390/122‑1 (MQ=255) catcCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAg < 2:208859/172‑1 (MQ=255) gCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTc < 2:267739/67‑1 (MQ=255) cGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGCTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGGACAAAGCATAAAACAAAGACCGTGGTTAGATTCGCTGATCGGTGGCAGCATTGCCGCTAACtag > 2:292197/1‑272 (MQ=255) aTAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGCAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCTTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAaacaac > 1:226789/1‑214 (MQ=255) aaggACAATGTATCGCCACGCACGGCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggttggt < 1:292202/272‑1 (MQ=255) aTGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATAc > 2:224224/1‑127 (MQ=255) cAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGa < 1:224226/127‑1 (MQ=255) cacgcacgTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAg > 2:73512/1‑178 (MQ=255) tcacgTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCgcagc < 1:68881/274‑1 (MQ=255) cacgTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATa < 1:73513/178‑1 (MQ=255) cgTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGcaca < 1:460179/275‑1 (MQ=255) ccACCCCTTACCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCt > 2:465566/1‑93 (MQ=255) cccTTACCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGcaa < 1:465573/93‑1 (MQ=255) tCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCTCGGAACTGCTCGGCCAGCTCGCGCATCCTGGCGTGCGAGGTACCAAGCATAACACAACGACCGTTCGTCACTTCCACGGACCGTCCCAGCATTGCCGCTACCTTGCGCGCAGACCGTGGCTGGCTGGTTTGCGGGNGAGTtcgccggnnnnacac > 2:27411/1‑258 (MQ=255) tCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCgc > 2:342318/1‑201 (MQ=255) | CGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCATCCGGCGAGGTAAACGGCAATTTGTCGATAATTACCAATGACAATGTATCGCCACGCACGTCCACCCCTTCCCAGAAACTGCTGGTTGCCACAAGAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAG > CP009273/1885666‑1886193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |