| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,936,504 | G→T | 13.8% | L113I (CTA→ATA) | znuA ← | zinc transporter subunit: periplasmic‑binding component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,936,504 | 0 | G | T | 13.8% | 82.7 / 4.3 | 29 | L113I (CTA→ATA) | znuA | zinc transporter subunit: periplasmic‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (13/12); new base T (2/2); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01 | |||||||||||
CGCGAGCTCGTTACCAACCTGCGTTTCGGTTGAGGCTAATTGTGCCTCAAAATCCTTCAGGTTGGCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGTGAATAATCATGTTCTGAAGCGCCGTCAGGAAGTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAAC > CP009273/1936253‑1936759 | cgcgAGCTCGTTACCAACCTGCGTTTCGGTTGAGGCTAATTGTGCCTCAAAATCCTTCAGGTTGGCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTgc > 1:252830/1‑274 (MQ=255)cgcgAGCTCGTTACCAACCTGCGTTTCGGTTGAGGCTAATTGTGCCTCAAAATCCTTCAGGTTGGCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGATGTAGCCCGCGCTCTCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTTATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCTTGTTCATCATCCTCTCAGTGACTACTTTTCATCAGTAGAGGTTTCACATCTTCAAGATg > 1:414057/1‑273 (MQ=255) tcccAACTTGGTTTTCGGTTGAGGTTAATTGTGCTTCAAAATCTTTCAGTTTGGCGTCAAGTTTGGCTCGACTTTGCGGCANAAGTTCCCNTAATTTTCCATGCATTGCAACCGCTGTAGCCCGCGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTAc < 1:483082/272‑1 (MQ=255) gggggTTTTGGTTGTGGTTAATTGTGGATCAAAATCCTTCAGGTTGNCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTATCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCg < 2:457422/271‑1 (MQ=255) tGCGTTTCGGTTGAGGCTAATTGTGCCTCAAAATCCTTCAGGTTGGCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCg < 2:252827/274‑1 (MQ=255) gAGGCTAATTGTGCCTCAAAATCCTTCAGGTTGGCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTGTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTATCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGc > 1:457429/1‑258 (MQ=255) ctgtGTAGCCCGCGTTATTTGTGGGGATTTCCAATGTTGCGTGGTGAAATCGCCTTGATTGTGATCTTCGTCTCTTTGTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCCATAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGATTTACATCCGATGGGCGCa < 1:581451/272‑1 (MQ=255) cgcgCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGt > 1:709197/1‑123 (MQ=255) cTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTca < 2:709183/123‑1 (MQ=255) aGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGTGAATAATCATGTTCTGACGCGCCGt > 1:169398/1‑274 (MQ=255) aattcgtcgTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTAGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGTGAATAATCATGTTCTGAAGCGCCGTCAGGAAGTAAAACCtctgt < 1:653398/272‑1 (MQ=255) aaaTCGCCGTGATTGTTATCTTCGTCACTTTTTTCCGCGTTGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGTGAATAATCATGTTCTGAAGCGCCGTCAGGAAGTAAAACCtctgt < 1:171043/275‑1 (MQ=255) cTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGGGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGTGAATAATCATGTTCTGACGCGCCGTCCGGAAGTAAAACCtctgtttc > 1:417424/1‑259 (MQ=255) ttttCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGc > 2:73887/1‑72 (MQ=255) gacaccGCTTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCgg > 2:297037/5‑116 (MQ=255) ccGCTTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGtttt < 1:297042/116‑1 (MQ=255) ccGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTgcg < 1:73888/72‑1 (MQ=255) cgTGGTCGTGATCATCATCATCGCCGGGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAATCTGCGCAATCGTTACCTGCTTCGCTACTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGACCAACCCAAACGACTAATTCCCCGtt > 2:89270/1‑163 (MQ=255) gTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGt < 1:89271/163‑1 (MQ=255) catcatcGCCGTGAATACTTTTCATCAGTATCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGc > 1:634099/1‑156 (MQ=255) catcatcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTAcc > 2:263171/1‑65 (MQ=255) catcatcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGTGAATAATCATGTTCTGAAGCGCCGTCAGGAAGTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTaagc > 1:17019/1‑275 (MQ=255) atcGCCGTGAATACTTTTCATCAGTATCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGttt < 2:634085/156‑1 (MQ=255) atcGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCt < 1:263174/65‑1 (MQ=255) cGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGtttt > 2:182544/1‑155 (MQ=255) gTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACAt < 1:182546/155‑1 (MQ=255) tGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGTGAATAATCATGTTCTGAAGCGCCGTCAGGAAGTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGATTAAGCGAAGCGACAAc > 1:89256/1‑275 (MQ=255) aaTACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGGGAATAATCATGTTCTGAAGCGCCGCCAGGAAGTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCAATGAACCCAACGGGCTTCAGCGAACCGACacc > 1:130269/1‑271 (MQ=255) cTTTNCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGGCGTGCCTAATCATCTTCTGAAGCGCCGTc < 1:514545/188‑1 (MQ=255) | CGCGAGCTCGTTACCAACCTGCGTTTCGGTTGAGGCTAATTGTGCCTCAAAATCCTTCAGGTTGGCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTTTTCCGCGTGGTCGTGATCATCATCATCGCCGTGAATACTTTTCATCAGTAGCGGTTTCACATCTTCAAGCTGCGCAATCGTTACCTGCTTCGCTCCTGGTAATTTGCTTACCGGTTTTTGCATAAACGCTTCCATCTCCGGGCCAACCCAAACGACTAAGTCCGCGTTCTGTAAGCGTTTTACATCCGATGGGCGCAGTGAATAATCATGTTCTGAAGCGCCGTCAGGAAGTAAAACCTCTGTTTCTGTTACCCCATCAGCAATGGCAGAAGCGATGAACCCAACGGGTTTAAGCGAAGCGACAAC > CP009273/1936253‑1936759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |