| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,912,731 | G→T | 100% | Q163K (CAG→AAG) | atpA ← | F1 sector of membrane‑bound ATP synthase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,912,731 | 0 | G | T | 100.0% | 117.4 / NA | 36 | Q163K (CAG→AAG) | atpA | F1 sector of membrane‑bound ATP synthase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (19/17); total (19/17) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGCAACCGGCATACGGTGCCAGGTATTGCAGTGCAGCGGATTCAGACGCGGTTGCTACCACAACGATGGTGTTAGCCAGTGCGCCGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTGCACCCAGAGTGTTAACCACACGGCCCAGCAGGCCACGGCCAACCGGAACTTCCAGGATACGGCCAG > CP009273/3912487‑3912946 | gcgaacacGGCATCCGGGGCCAGTTAGTGCAGTGCAGCTGATTCAGACGCGGTTGCTTCCACAACGATGGTGTTAGCCAGTGCGCCTTGCTCTTACAGGTTACGTACCACGTTAGAAATGGTGGTCGCTGTCTGGCCGATAGGGACATAGATACATTTGATACTGGAATCGCGCTGGCTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTTTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCacc < 2:730517/268‑3 (MQ=255) gcagCGGATTCAGACGCGGTTGCTACCACAACGATGGTGTTAGCCAGTGCGCCGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGAc > 1:160283/1‑220 (MQ=255) gCGGATTCAGACGCGGTTGCTACCACAACGATGGTGTTAGCCAGTGCGCCGTGCTCTTCCANTTTACGTANCACGTTAGAAATTGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGTATCGAAAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGCTTGGGATCATGGAGTCAACGGCTTTATAACCGGt < 1:482588/254‑1 (MQ=255) cGGATTCAGACGCGGTTGCTACCACAACGATGGTGTTAGCCAGTGCGCCGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGAt < 2:160281/220‑1 (MQ=255) aTGGTGTTAGCCAGTGCGCCGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCttt > 2:471874/1‑215 (MQ=255) tgtTAGCCAGTGCGCCGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATaa < 1:471882/215‑1 (MQ=255) aGCCAGTGCGCCGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCAGGAGCGATTGCTTCTAcag > 1:363772/1‑275 (MQ=255) ccAGTGCGCCGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTAc > 2:365761/1‑171 (MQ=255) tGCGCCGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACg < 1:365768/171‑1 (MQ=255) tGCGCCGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCg > 2:579534/1‑175 (MQ=255) tGCGCCGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATCGGGATCATGGAGt > 1:411238/1‑191 (MQ=255) ccGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTg < 1:579547/175‑1 (MQ=255) ccGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAAc < 2:411231/191‑1 (MQ=255) cGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGc > 1:413093/1‑250 (MQ=255) aCGTTAGAAACGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGAc > 1:717376/1‑145 (MQ=255) tAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGAt < 2:717362/145‑1 (MQ=255) aGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGCCCGATTGGGATCATGGAGTCAACGGCTTTATAATCGGTGTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAAAGCAGAGAAGCCGTCGTGAACCAGCGGACATTTACCGTCGa > 1:389342/1‑275 (MQ=255) gtggACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGATGCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTGc > 1:587080/1‑275 (MQ=255) gtggACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTg > 1:285941/1‑274 (MQ=255) acTTTCTTGACGAGAGCGACATAGATACAGTTGATACCGGAATCGCGCTGGTTGATGGTGGCATCGAGAGCCAGTGCGGTTTCGACTGGCTGACGGTCACCGATGATCAATGCACGCTTACCACGACCGATGGGGATCATGGAGTCAACGGCTTGATAACCGGTCTGTAACGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTGCACCCa < 2:189401/273‑1 (MQ=255) aTAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGa > 1:456051/1‑250 (MQ=255) tggtgacGGNGTCGGGCTGTTGTATTATTGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATAAATTCACGCTTAACACGACCGATTGGTGTCATGGAGTCAACGGCTTTCTAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTGCACCCAGAGTGTTAACCACACGGCCCAGCAGGCCACgg < 2:285936/269‑1 (MQ=255) tCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTc < 1:196326/123‑1 (MQ=255) gCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTAcc > 1:88589/1‑62 (MQ=255) gCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGAt > 2:236224/1‑71 (MQ=255) gCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCTGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGGCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTTCACCCAGAGTGTTAACCACACGGCCCAGCAGGCCACGGACAACCGGAACATACAGAATACgacaag > 1:257764/1‑269 (MQ=255) gCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGtt > 2:649824/1‑133 (MQ=255) gCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTAGTGCACCAGCGGACCTTTACCGt > 1:181404/1‑199 (MQ=255) cGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGa < 2:88588/62‑1 (MQ=255) cGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTggg < 1:236226/71‑1 (MQ=255) cGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGAt < 1:649838/133‑1 (MQ=255) aGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCttt > 1:211512/1‑83 (MQ=255) cGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATaa < 2:211510/83‑1 (MQ=255) aCGGTCACCGATGATCAATTCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTGCACCCa < 1:117308/182‑1 (MQ=255) ttCACGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTta > 1:402360/1‑46 (MQ=255) cGCTTACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAAc < 2:402353/46‑1 (MQ=255) | GCGCAACCGGCATACGGTGCCAGGTATTGCAGTGCAGCGGATTCAGACGCGGTTGCTACCACAACGATGGTGTTAGCCAGTGCGCCGTGCTCTTCCAGTTTACGTACCACGTTAGAAATGGTGGACGCTTTCTGGCCGATAGCGACATAGATACATTTGATACCGGAATCGCGCTGGTTGATGATGGCATCGATAGCCAGTGCGGTTTTACCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTGCACCCAGAGTGTTAACCACACGGCCCAGCAGGCCACGGCCAACCGGAACTTCCAGGATACGGCCAG > CP009273/3912487‑3912946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |