Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,921,929 C→T 8.4% L345L (CTG→CTA)  viaA ← stimulator of RavA ATPase activity; von Willebrand factor domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,921,9290CT 8.4% 147.8 / 4.9 51L345L (CTG→CTA) viaAstimulator of RavA ATPase activity; von Willebrand factor domain protein
Reads supporting (aligned to +/- strand):  ref base C (22/23);  new base T (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

GAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAG  >  CP009273/3921691‑3922174
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     
gcgcgagcaaaTCCGAAATACCCGCCGCGATGCCATTAAACCAGTTCCTGCTATGCAAGCGTTCCCTAATGGCGCGAAAACAACTGGCAAGATCGGGGCCGCCACGAAACTGCTGGCTTAAAACACGGCGTGCTGGTTCGATGCCTTGTGAGACTGACGGCTCCTAACGGTCGGTCTAGGTGGAAAATAGCATAATATAGCAGGGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGTCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAgccg                                                                                                                                                                                                                   <  2:247143/267‑1 (MQ=255)
                                                             ttCCATAATTGCGCGAAAACAACTGGCAAGTTCGGTGCCGCCATGAAACTCGTCGCTTAAAAATCGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAAt                                                                                                                                                      <  1:278677/275‑1 (MQ=255)
                                                             ttCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGAATAATATAGCAGCGCCGGTTTTCTGCGAGAGGAATGAGCATCAAGGCCAGGCAGAACGCTTTCGCAACCTGTGCATTAAAGCCGCCCATTGCACCGGAAGAACACAAACCAAAAAAAACCGACACGCCCGGCAGTTCAACGTaa                                                                                                                                                       >  2:315011/1‑274 (MQ=255)
                                                               ccATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCAGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCttt                                                                                                                                                                                                                                         >  2:36618/1‑190 (MQ=255)
                                                                   aaTGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCAGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGca                                                                                                                                                                                                                                     <  1:36619/190‑1 (MQ=255)
                                                                     tGGCGCGAAAACAACTTGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGANAATAGCATAAGCTATCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATg                                                                                                                                               <  1:686281/274‑1 (MQ=255)
                                                                         gcgAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCg                                                                                                                                                                                                                                       >  1:624058/1‑182 (MQ=255)
                                                                          cgAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGc                                                                                                                                                                                                                                                   >  1:213579/1‑169 (MQ=255)
                                                                             aaaCAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGcaca                                                                                                                                                                                                                                   <  2:624044/182‑1 (MQ=255)
                                                                              aacaacTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGaa                                                                                                                                                                                                                                               <  2:213577/169‑1 (MQ=255)
                                                                                                tGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAGTATAGCAGCGCCGGTTGTGTGCGAGGGAAATGCGCATCAAAGCCAAGCAGAACGCTTTCGCACACTGTTCATTAAATCCGCCCATTGAGCCGGAAGTATCCAAACAGAAAATAAACGGCGCAAGCGGCTGCTCAGCGTAATCATAATGTCTCACTGCACGTGCCAACACttcttc                                                                                                                    >  2:43523/1‑274 (MQ=255)
                                                                                                tGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCANTGAGCCGGNAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCa                                                                                                                   >  1:482823/1‑275 (MQ=255)
                                                                                                  ccgccACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTAATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACg                                                                                                                 <  1:315016/275‑1 (MQ=255)
                                                                                                     ccACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCTTTGCGACGGAAGTATcc                                                                                                                                                                                              >  1:391257/1‑195 (MQ=255)
                                                                                                          aaaCTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCAAGGCAGAACGCTTTCGCACACTGTTCAt                                                                                                                                                                                                                           >  2:406920/1‑161 (MQ=255)
                                                                                                              tgctgGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTaaa                                                                                                                                                                                                                       <  1:406927/161‑1 (MQ=255)
                                                                                                                                    cTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCTAGGCAGAACGCttt                                                                                                                                                                                                                                         >  1:215285/1‑121 (MQ=255)
                                                                                                                                        ttCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCTAGGCAGAACGCTTTCGca                                                                                                                                                                                                                                     <  2:215283/121‑1 (MQ=255)
                                                                                                                                          cGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCa                                                                                                                                                             >  1:73560/1‑191 (MQ=255)
                                                                                                                                              gCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCTCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGt                                                                                                                                                         <  2:73559/191‑1 (MQ=255)
                                                                                                                                                  tgtgGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCttt                                                                                                                                                                                                                                         >  1:271511/1‑107 (MQ=255)
                                                                                                                                                   gtgGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGACCCGCGCGGCTGGTCATCGTAATCTTTCTGTACCACCGGACGTTCGATCCCTTTTTCACGACACGACTCACCGTGCAGGCGAAAGGTGAGCAATGATCTTTCCACAAGc                                                                >  1:182057/1‑275 (MQ=255)
                                                                                                                                                      ggCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGca                                                                                                                                                                                                                                     <  2:271507/107‑1 (MQ=255)
                                                                                                                                                       gtaaagaaggttaTAAAGGACGATANGGGTGGAAAATAGCATAATATAGCGGCGTCGGTTTTCTTCTAGAGCAATGCGCATCAAGGCGAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGa                                                             <  2:482814/262‑1 (MQ=255)
                                                                                                                                                       gCCTGAAAGCTAATAACGGACGATCTCGGTGGAAAATAGCATAATAGAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGNAAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATCGGTGAGCAACTGTTTTTCCACCAGCCGa                                                             <  1:563191/274‑1 (MQ=255)
                                                                                                                                                                      aCGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGcc                                                                                                                                                                                                                                                       <  1:261877/73‑1 (MQ=255)
                                                                                                                                                                          aCGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAg                                                                                                                                                                                                                                                 >  1:71695/1‑75 (MQ=255)
                                                                                                                                                                          aCGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATcc                                                                                                                                                                                              >  2:616590/1‑126 (MQ=255)
                                                                                                                                                                           cGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCAGCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCAtcacacag                                         >  1:389784/1‑267 (MQ=255)
                                                                                                                                                                              tctcGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACg                                                                                                                                                                                                                                             <  2:71694/75‑1 (MQ=255)
                                                                                                                                                                              tctcGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCcacac                                                                                                                                                                                          <  1:616603/126‑1 (MQ=255)
                                                                                                                                                                                              cATAATATAGCAGCGCCGGTTATCTGCGTAAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAg                                                                                                                                                                                                   >  1:524703/1‑101 (MQ=255)
                                                                                                                                                                                                  atatAGCAGCGCCGTTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACCCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCATACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATg                                                                                                                                               <  1:473784/149‑1 (MQ=255)
                                                                                                                                                                                                  atatAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATc                                                                                                                                                                                               <  2:524694/101‑1 (MQ=255)
                                                                                                                                                                                                        cagcGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTa                                                                                                                                                        >  2:454905/1‑134 (MQ=255)
                                                                                                                                                                                                        cagcGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGa                                                                                                           >  2:682538/1‑179 (MQ=255)
                                                                                                                                                                                                           cgcCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGcac                                                                                                                                                                                                                                    >  2:131509/1‑55 (MQ=255)
                                                                                                                                                                                                           cgcCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAgccgc                                                                                                                                                                                                                  >  1:423074/1‑73 (MQ=255)
                                                                                                                                                                                                            gcCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCt                                                                                                                                                    <  1:454912/134‑1 (MQ=255)
                                                                                                                                                                                                            gcCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCa                                                                                                       <  1:682552/179‑1 (MQ=255)
                                                                                                                                                                                                             ccGGTTTTCTGCGAGAGCAATGCGCATCAAGGCTAGGCAGAACGCTTTCg                                                                                                                                                                                                                                       >  1:458520/1‑50 (MQ=255)
                                                                                                                                                                                                             ccGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCttt                                                                                                                                                  >  2:621646/1‑135 (MQ=255)
                                                                                                                                                                                                             ccGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGAAACTGCTTTTCCACCAGACGACGGAAAAACTCATACGCCAGTTCCGTTCTCCATCGTTTCGCCAGTTTTggcgga       >  2:687448/1‑273 (MQ=255)
                                                                                                                                                                                                             ccGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACAACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATCGTCCAGTTCCATTATCCCTAGTTTCGACAGTNCTagcgccc      >  2:270740/1‑272 (MQ=255)
                                                                                                                                                                                                               ggTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTg                                                                                                                                                                                                                                <  1:131510/55‑1 (MQ=255)
                                                                                                                                                                                                               ggTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCAt                                                                                                                                                                                                              <  2:423067/73‑1 (MQ=255)
                                                                                                                                                                                                                 ttttCTGCGAGAGCAATGCGCATCAAGGCTAGGCAGAACGCTTTCGcaca                                                                                                                                                                                                                                   <  2:458513/50‑1 (MQ=255)
                                                                                                                                                                                                                 ttttCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGTAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGgag  <  1:687462/275‑1 (MQ=255)
                                                                                                                                                                                                                 ttttCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGt                                                                                                                                              <  1:621660/135‑1 (MQ=255)
                                                                                                                                                                                                                 ttttCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGgag  <  1:270744/275‑1 (MQ=255)
                                                                                                                                                                                                                     cTGCGAGAGCAATGCGCATCAAGGCCAGTCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCcac                                                                                                                                                                                            >  1:558106/1‑85 (MQ=255)
                                                                                                                                                                                                                         gagaGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAg                                                                                                                                                                                        <  2:558094/85‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     
GAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAG  >  CP009273/3921691‑3922174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑