| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,285,744:1 | +G | 100% | coding (47/1014 nt) | rssB → | PcnB‑degradosome interaction factor; response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,285,744 | 1 | . | G | 90.9% | 57.2 / 2.1 | 22 | coding (47/1014 nt) | rssB | PcnB‑degradosome interaction factor; response regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (7/13); total (8/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATTCAACCTGTTTGCCCGCAAATATCTACGCTTGATTTCCATCGCGCGCACGCTGCCATTGCGGCCGGACAGCTGGCAGTGGAAAGGAAAATGGACGAACTTTTGCCGTTGGTACGCACCAACATTTGACCAGAATTTTTATCTACACTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑A‑GCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAA > CP009273/1285477‑1285984 | gATTCAACCTGTTTGCCCGCAAATATCTACGCTTGATTTCCATCGCGAGCACGCTGCCATTGCGGCCGGACAGCTGGCAGTGGAAAGGAAAATGGACGAACTTTTGCCGTTGGTACGCACCAACATTTGACCAGACTTTTTATCTACACTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCCCTATTGAGTAAAGCCAGTCAGGGGAGAGAAACTGCAGCAGCCATTGGTCGGAAAACAGATTCACATTGTTGAAAAT‑G‑A‑ggcaagt > 1:129155/1‑269 (MQ=255) gcGCTGCCATTGCGGCCGGACAGCTGGCAGTGGAAAGGAAAATGGAAGAACTTTTGCCGTTGGTACGCACAAACATTTGACCAGAAGTTTTATCTACACTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACg < 1:252980/273‑1 (MQ=255) ttcAGTCGAAAGGAAAAGTGTCAGACTTGTGCCTTTGGTAAGCACCAACATTTTTCCAATATTTTTATCTACACTTAAGGTAATTCTTACAGGTGCAGGTGGCAATAGCAGGCCACTATTGAGTAAAGCCAGTCAAGGGAGAGAACATGACGCCGCCATTGGTCGGAAAACAGATTCTCATTGTTGCAGAT‑G‑AGGCAGGTATGTCGGTCGCGTCGGGCATAATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGa < 2:129149/270‑1 (MQ=255) aCGCACCAACATTTGACCAGAATTTTTATCTACACTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGATCGGAGAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTgatgat < 1:434758/275‑1 (MQ=255) cATTTGACCAGAATTTTTATCTACACTTAAGTTACTTCTGACAGTCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAACCAGATTCTCATTGTTGAAGAT‑GCG‑GCCGGTATTtcgctcgc > 2:341099/1‑163 (MQ=255) tGACCAGAATGTTTAGCTACACTTAAGTTAATTATGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGCATAT‑G‑AGGCAGGTATTTCGCTCGCTTCt < 1:341111/163‑1 (MQ=255) aTCTACACTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCNTTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGaa < 1:378163/275‑1 (MQ=255) tGACAGGCGCATGTGGCAATAGCATGCCACTATTGAGTAAATCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCTACAACGGTACTGGCGGCTGATGTGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGCCCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGc < 1:316744/275‑1 (MQ=255) gCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGACCGGGCTTAAACTGCTGGAGCATATACGTAAcctggcgcttatac > 1:267315/1‑261 (MQ=255) aCTATTGAGTAAAGCCAGTCAGTGGAGAGAACATGACGCAGCCATTGGTAGGAAAAGAGAATCTCATTGTTGAAGAT‑G‑AGGCAGGTATGTCGCTCGGTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGGTGGGAGGTGTCACTCAAGACCTGATGATATGTGATATCGGGATGCCACGAATGAACGGGCTTAAActgc < 2:482744/241‑1 (MQ=255) cTATTGAGTAAAGCCAGTCAGTGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATCTCGCGATGcc < 1:479053/217‑1 (MQ=255) tATTGAGTAAAGCCAGTCCGGGTAGAGAACATGACGCAGCCATTGGTCGGAAAGCAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTAttt > 1:311563/1‑88 (MQ=255) gAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTtcgct < 2:311554/88‑1 (MQ=255) ccAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGatat > 1:288683/1‑275 (MQ=255) gTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTAttt > 1:263998/1‑73 (MQ=255) ggggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTtcgct < 2:263990/73‑1 (MQ=255) gACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGCGGTTTCAc > 1:306222/1‑151 (MQ=255) gACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTACTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGACTGAACGGGCTTACACTGCTGTAGCATATAGGTAACAGCGGCGAACCAAACCAAGTTCTGGTGATAGATGCCACTGAAAATATgg > 2:230372/1‑273 (MQ=255) cAGCAATTGGTCGGACAACAGAATCTCATTGTTGAAGATGG‑G‑GCCGGTATTTCGCGCGCTTCTGGATTCATGGTTTTCCTCATTCGGAGCGACAACGGTACTGGCGGATGATGGGGTGGATGTCCTTGAGTTGCTGGGAGGTTTCACTCCa < 2:306213/151‑1 (MQ=255) aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgggg > 1:99482/1‑115 (MQ=255) aTTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGa < 2:99480/115‑1 (MQ=255) ttgttggAAAACTGATTCTCATTTTTGAAGAT‑G‑AGGTAGGTATTTCGCTCGCTTCTGGAGTCATGGGTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCaa < 1:230379/270‑1 (MQ=255) tCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑AGGCAGGTATTTCNCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTTAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGACCATATGCCTAACAGAGGCGACCAGAcccc < 1:352162/227‑1 (MQ=255) | GATTCAACCTGTTTGCCCGCAAATATCTACGCTTGATTTCCATCGCGCGCACGCTGCCATTGCGGCCGGACAGCTGGCAGTGGAAAGGAAAATGGACGAACTTTTGCCGTTGGTACGCACCAACATTTGACCAGAATTTTTATCTACACTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGAT‑G‑A‑GCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTGATATCGCGATGCCACGAATGAACGGGCTTAAACTGCTGGAGCATATACGTAACAGAGGCGACCAGACCCCAGTTCTGGTGATATCTGCCACTGAAAATATGGCAGATATTGCCAA > CP009273/1285477‑1285984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |