| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,458,375 | G→T | 25.3% | E194* (GAA→TAA) | paaX → | transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,458,375 | 0 | G | T | 25.3% | 31.0 / 3.6 | 19 | E194* (GAA→TAA) | paaX | transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer |
| Reads supporting (aligned to +/- strand): ref base G (7/7); new base T (2/3); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01 | |||||||||||
GAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTGTCGTCCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGC > CP009273/1458141‑1458562 | gcctgTTTAGAAAAAGCAACGCTTGCTGATCTCAAACAGCAGTTGGTCTGGCAAGGTTTTGGCGCACTGGTACCCAGCCTGATGGTATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGAATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGGGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTccg < 2:82196/271‑1 (MQ=255) gCACCCAGCCTGATAGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCa < 1:373340/178‑1 (MQ=255) cATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTTGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGAc > 1:201067/1‑224 (MQ=255) ggcttCGCAAAAGCTGGTCGATGGACAGACACTTTTGCATGACGCGGGTGTGGCGGATAACGTGATTTGTTTTGAATCGCAAATACCACTGGCGCTTTCTGGCGCAGCACTGCGGGGCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGt < 2:201061/220‑1 (MQ=255) ttGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTTAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTGTCGTCCTTAAAGACCCATTGTTGCCgg > 1:89803/1‑274 (MQ=255) cGGTTAACGTGGTTTGTTTTTAAGCGCAAATAACACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTTAACAAAATGCCATGTACGAAACCTTTATTCAGTCAATCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTGTCGTCCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGcaca < 2:89801/274‑1 (MQ=255) aaCGTGATTTGTTTTAAAGCGCAAATAAAACTGGCGCTTTCTCGCGCAGAACTGCGGGCCAGAGTAGAAGAGTGCTGGCATTTGAATGAACAAAATGAAATGTACGAAACATTTATTCAGTCATTAAGCCCGCTGGTGCAGCTTGTAAACGAGGCGACAGACGAGTTAACACCGGAGCGGGCATTTCATATTCAGCAGTTACAACTCCACTTTTATCGCCGTGACGTCCTGAAAGCACCCTTGTTTCCGGAGGCGTTGGTTCCGGCACACtaaga > 2:70224/1‑271 (MQ=255) aCTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGcc > 1:216268/1‑191 (MQ=255) gcgcTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCgtgt < 2:216261/191‑1 (MQ=255) caCTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTTAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTgg > 2:401371/1‑87 (MQ=255) gCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTTAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGcc < 1:401384/87‑1 (MQ=255) agTAGAAGAGTGCTGGCATTTAACTGAACAAAATGTCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCAGCTTTTAAAAGaggagg > 1:61738/1‑95 (MQ=255) gaagaGTGCTGGCATTTAACTTGACAAAATGCCATGTACGAAACCTGTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGa < 2:61736/95‑1 (MQ=255) aagaGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTa > 1:227136/1‑48 (MQ=255) gTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCa < 2:227129/48‑1 (MQ=255) tGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTGTCGTCCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGcaca > 1:173257/1‑197 (MQ=255) ggCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTGTCGTCCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTgg < 2:173251/197‑1 (MQ=255) gCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTgg > 2:312952/1‑59 (MQ=255) ttAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGcc < 1:312961/59‑1 (MQ=255) | GAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTGTCGTCCTTAAAGACCCATTGTTGCCGGAGGAGTTGCTTCCGGCACACTGGGC > CP009273/1458141‑1458562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |