Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,514,467	T→C	10.6%	intergenic (+146/‑52)	curA → / → mcbR	curcumin/dihydrocurcumin reductase, NADPH‑dependent/colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,514,467	0	T	C	10.6%	94.8 / 4.5	38	intergenic (+146/‑52)	curA/mcbR	curcumin/dihydrocurcumin reductase, NADPH‑dependent/colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled
Reads supporting (aligned to +/- strand):  ref base T (18/16);  new base C (2/2);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

ACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATGAGTATTACACCTGTGCGTGAAGCATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGC  >  CP009273/1514209‑1514718                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                            aCTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTGACTAACCACATAATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATgcag                                                                                                                                                                                                                                             >  1:266871/1‑275 (MQ=255)      cgcgAATAAATTTCTGTCGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCTGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATgcaggca                                                                                                                                                                                                                                          <  1:472982/273‑1 (MQ=255)                                                       gCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGaaaaa                                                                                                                                                                                       >  1:46920/1‑275 (MQ=255)                                                               aaGGGTAAACACTTGGGCAAAGTGGTGATCCGCGTGGCGGGTGATGAATAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACAt                                                                                                                                                                               <  1:108992/275‑1 (MQ=255)                                                                                        tgcaccGCGTCNCAGGTGATGCTTAAAGTTTCCACGGCTGCGTAAGCCGCCATAAAAATCCATGCTAATAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTTTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCATGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAGCATGTCAGTTTGACTCTTCAGGTTgaga                                                                                                                                                      <  2:46918/271‑1 (MQ=255)                                                                                          ttcatcgcgggctgTGATGATTCAAATTTCAACTGTGGCTTAAGCAGCAATGAAAATCTAAGATAATAGATTAATTTCAGCTATCATTAACGAACCACATGTATTTTTGGCTGATAATAACAACCAATTTGAGTTGTTTATGATTAGTCGCTTTAATACAGCAGGTGAATTCCTTCTTCCAAGCATGCAGGGTTTGACAGAAAACACTCGAAGGAATTAAAATGCCTTGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCACGTTGAGaa                                                                                                                                                     <  2:476590/261‑1 (MQ=255)                                                                                                          atgatTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGc                                                                                                                                    <  1:270690/275‑1 (MQ=255)                                                                                                                                                      ggCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCt                                                                                                                                                                                                                                                         >  2:65669/1‑114 (MQ=255)                                                                                                                                                          aaaaGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTg                                                                                                                                                                                                                                                     <  1:65671/114‑1 (MQ=255)                                                                                                                                                           aaaGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCAATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATAAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACATGGCGACCGCCAAATTACTAAAAATCt                                                                                   >  1:56122/1‑275 (MQ=255)                                                                                                                                                                 tgagaaaggatttANTTAACTTACCAAATCATTGTTCTGATGCTAATAACAACAAAGGTGAGTTGTTTATGATTATTCACTGTAAGACACCAGGTGAACTCCTTCTGCCATGAAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGa                                                                             <  2:357679/265‑1 (MQ=255)                                                                                                                                                                             taattaaCTAACCTCATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTAACTTTAATACACCAGGTGAATTCCtt                                                                                                                                                                                                                                                        <  1:326452/92‑1 (MQ=255)                                                                                                                                                                              agagacagaacCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCACGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTCAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATg                                                                <  1:493144/267‑1 (MQ=255)                                                                                                                                                                              aattaaCTAAACAAATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAAGACACCAGGTGAATTCCTTCTGCCACGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATg                                                                <  1:278765/275‑1 (MQ=255)                                                                                                                                                                                    ctaacCACATCATTGTTCTGCTGCTAATGACAACAAAGGTGAGTAGTTTATGATTATTCACTTTAATACACCAGGTGAACTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTATGACTCTGCAGGTTGAGANCGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATAAGGTaatttctct                                                          >  1:357691/1‑267 (MQ=255)                                                                                                                                                                                        ccACATCATTGTTCTGCTGCTAATTACAATAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGANCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATGAGTATTacaa                                                      >  1:346620/1‑274 (MQ=255)                                                                                                                                                                                              catTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGCTGACTAAAAATTTGGGGGGGAGGTTAGGTATGAAGATTACAccgtttc                                                >  2:241693/1‑270 (MQ=255)                                                                                                                                                                                                 tGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATg                                                                                                                                                                                                                                                >  2:358543/1‑80 (MQ=255)                                                                                                                                                                                                 tGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAACATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGAACTGAAACATAAGCTTAGTATTGGCGCATTAAAAACGGGCGCACGCCTGATTACTAAAAAACTGGCGGAGCAATTAgg                                                                    >  2:493131/1‑252 (MQ=255)                                                                                                                                                                                                     ctgctgCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATgcagg                                                                                                                                                                                                                                            <  1:358555/80‑1 (MQ=255)                                                                                                                                                                                                                          gTGAGTGGTTTATGATTATTCACTGTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAANCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAg                                                                     >  1:349331/1‑226 (MQ=255)                                                                                                                                                                                                                             aGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATc                                                                                                                                       >  2:81479/1‑157 (MQ=255)                                                                                                                                                                                                                                 gTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCt                                                                                                                                   <  1:81481/157‑1 (MQ=255)                                                                                                                                                                                                                                            ttCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCATGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAGACCTGGCGCACGCCTGATTNCTAAAAATCTGTCGGAGCAATTAGGTATGCGTATTACACCTGTGCGTGAAGCATTATTACGTCTGGTTTCTGTGAATGCGCTTTCTGTCga  >  1:389836/1‑274 (MQ=255)                                                                                                                                                                                                                                            ttCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAacac                                                                                                                                                                                                                        >  1:132252/1‑61 (MQ=255)                                                                                                                                                                                                                                            ttCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTaaaa                                                                                                                                                                                                         >  2:79569/1‑76 (MQ=255)                                                                                                                                                                                                                                            ttCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATAAAAACCTGGCGCCCGCCTGATTACTCAAAATCTGGCGGAGCACTTAGGGATGAGAAATACACCTGTGCGTGAAGCATTATTACATCGGGTTTCGGTGAATGCGGTTTgtgtgcc  >  2:485621/1‑272 (MQ=255)                                                                                                                                                                                                                                                cTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGa                                                                                                                                                                                                                    <  2:132246/61‑1 (MQ=255)                                                                                                                                                                                                                                                cTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGcc                                                                                                                                                                                                     <  1:79571/76‑1 (MQ=255)                                                                                                                                                                                                                                                 tttAATACACCAGGTGAACTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAgg                                                                    >  1:277809/1‑204 (MQ=255)                                                                                                                                                                                                                                                     aTACACCAGGTGAACTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATg                                                                <  2:277801/204‑1 (MQ=255)                                                                                                                                                                                                                                                           cAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAacac                                                                                                                                                                                                                        >  2:178986/1‑46 (MQ=255)                                                                                                                                                                                                                                                           cAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACg                                                                                                                                                   >  1:297601/1‑115 (MQ=255)                                                                                                                                                                                                                                                           cAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTa                                                                                                                              >  1:41653/1‑136 (MQ=255)                                                                                                                                                                                                                                                               tggaTTCCTTCTTCCATGCAGGCAGGTTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGCAAAATGAAACACGTCAGTTTTACTCTGCAGGTTGAGACCGACCt                                                                                                                                               <  2:297592/112‑1 (MQ=255)                                                                                                                                                                                                                                                               tGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGa                                                                                                                                                                                                                    <  1:178992/46‑1 (MQ=255)                                                                                                                                                                                                                                                               tGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTgg                                                                                                                          <  2:41652/136‑1 (MQ=255)                                                                                                                                                                                                                                                                 aaTTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAgg                                                                    >  1:403215/1‑188 (MQ=255)                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                            ACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACCTGAAACATCAGCTTAGTATTGGCGCATTAAAACCTGGCGCACGCCTGATTACTAAAAATCTGGCGGAGCAATTAGGTATGAGTATTACACCTGTGCGTGAAGCATTATTACGTCTGGTTTCGGTGAATGCGCTTTCTGTCGC  >  CP009273/1514209‑1514718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑