| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,128,324 | G→T | 12.6% | A270A (GCC→GCA) | wzc ← | colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,128,324 | 0 | G | T | 12.6% | 89.8 / 2.1 | 32 | A270A (GCC→GCA) | wzc | colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (13/15); new base T (2/2); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01 | |||||||||||
GCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTAAACT > CP009273/2128101‑2128538 | gcagcGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGa < 1:173226/275‑1 (MQ=255) tGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTGCTGCTCTTCCGCGTCTTTTCGATCAATATTTCGtgtcttttttttt > 2:274986/1‑263 (MQ=255) aCGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACAGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCg < 1:274994/275‑1 (MQ=255) tACAGCTTGGAGATTTCCGTCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACAACCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCCCCGATTTGCGCTCAATATTTTGTTCCTGATATTTACGGGCGATGCTGTTAAGAATGTcgc > 1:401964/1‑274 (MQ=255) aGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGAc > 1:403660/1‑204 (MQ=255) tGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCtt < 2:403647/204‑1 (MQ=255) ctctTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACTGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTNGTTTTCGGCAAGATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCAc < 1:455795/275‑1 (MQ=255) atatttAAAGGTATGGTAGTTCAATTGCGCATCGATGTTCACCATCGAATCGTGCACCGCTTTTGCTTCCAGGGGCAGATCAACAGAATCTTTAGCCGGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCGGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCAc < 2:401951/272‑1 (MQ=255) cgcATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCt > 2:181087/1‑244 (MQ=255) tCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCAc < 1:181093/244‑1 (MQ=255) ttaCCTTCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGTTCAACAGAATCTTTATCCTGACGGAAGGCATTCATTTTGTTTTCGGCAACATCCAATCGGCTACGTACTTCCGGTAACTGTTGCGCGATGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGAGAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCCAGCGTCTTTGCCg < 1:269288/273‑1 (MQ=255) cACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTc > 2:353129/1‑180 (MQ=255) atcgaatcgaGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGa < 1:353141/180‑1 (MQ=255) aGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTg > 1:36139/1‑150 (MQ=255) ccGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCt < 2:36138/150‑1 (MQ=255) tCCAGCGGCAGATCAACAGAAGCGTTATTCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCAGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGNCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCAAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCa > 1:337965/1‑274 (MQ=255) cAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGAt > 2:280152/1‑149 (MQ=255) tCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGtt < 1:280160/149‑1 (MQ=255) cAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGTCTCAAACCGCCAGCGTCTTTGCCGTTCTCCGTTACCGCCAGGCTGTTTTTCAgt > 1:8673/1‑260 (MQ=255) aaTCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAATGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGACTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTCGCAGTTGGTTGATCATccccctc > 2:485938/1‑272 (MQ=255) ttATCATGACGGAANGCCTTCTGTTTGTTGTCGGCAACATCCAACCCGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGa < 2:362188/275‑1 (MQ=255) aCGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATANGTCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCg > 1:362200/1‑263 (MQ=255) taGTTTGTTTTCGGCAACATCCAAGCGGTTACGTACTTCCGGTAACTGTTGCGCGAGGAATGCGAGGCTTTTCGACGCTGCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTAACGGTAAACt < 1:485951/274‑1 (MQ=255) aCATCTAAGCGTTTACGTACTTCCGGTAACTGTTGTGCGAGGATGGAGAGGCTATTCGAAGCTTTCGCCGAGTTGCGCTCAATATTTTGtt < 1:270154/91‑1 (MQ=255) gCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTcgc > 1:493633/1‑119 (MQ=255) cTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGAt < 2:493620/119‑1 (MQ=255) tACTTCCGGTAACTGTTGCGCGAGGAATGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACAGGCGa > 2:63328/1‑92 (MQ=255) tCCGGTAACTGTTGCGCGAGGAATGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACAGGCGATGCt < 1:63330/92‑1 (MQ=255) gTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGc > 1:225484/1‑82 (MQ=255) cTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATg < 2:225477/82‑1 (MQ=255) tGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTg > 2:232889/1‑45 (MQ=255) gcgcGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCt < 1:232896/45‑1 (MQ=255) | GCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTAAACT > CP009273/2128101‑2128538 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |