| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,482,690 | G→C | 11.5% | intergenic (‑43/+31) | yfdE ← / ← yfdV | acetyl‑CoA:oxalate CoA‑transferase/putative transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,482,690 | 0 | G | C | 11.5% | 98.0 / 2.1 | 32 | intergenic (‑43/+31) | yfdE/yfdV | acetyl‑CoA:oxalate CoA‑transferase/putative transporter |
| Reads supporting (aligned to +/- strand): ref base G (14/14); new base C (2/2); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATAC > CP009273/2482438‑2482920 | gCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTAAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAATGTCCATTAATGACATGTGTAATAGCGATAACTAATAAGCCATCAAAAGGACTTTTTCTTTCATTATTTGTAATTTGCTTTAGCCTTGAAAAAAACCCAAGGTGAAAAGACACTTCCACAATTATATaaaaataaaaac > 1:265614/1‑267 (MQ=255) tccatccaCATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGTCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAACAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAATCGACTAACATa > 1:468310/1‑274 (MQ=255) gACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGATTTCATTATTTNTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACAAGTAGACTGACATAAATCAACAAGGAAg > 1:455651/1‑274 (MQ=255) aCCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAAATAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATGTATGTAAAATCAATAAAATGAAAAATTATGAAACCAGTCGACTGACATACATcccnactgtacct > 2:192687/1‑264 (MQ=255) aaTGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGAc < 1:192693/252‑1 (MQ=255) tgCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTAGTTGTCCTTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTgacta > 1:430592/1‑272 (MQ=255) atcaCCATTACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAAAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTACTTATACCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATAATGAAACCAGTCGACTGAAATAAATCAAAAAGGGAACCGTGACGAaaaacacaaga > 1:433495/1‑268 (MQ=255) gcggaTCAACTTTAATTACCCTTTCCACCATATTACAAAAAAGTTGAGTTCCGAAAGGTCCATTAAGTATATGTTTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTTACGACAAAGCCCAGTATGCTCACAGCCaa < 2:433482/275‑1 (MQ=255) taaaCGTTAATTAGTCTGGAGTCCATTTTACAAACAATTTGAGTTCCGAAATGGACATTTAGGACATTTGTCATATCGATAACTAATATGCCTTCAAACTGCCCTTAGCTTTCATTATGTGTTATTTGCGTATGCCGTGAAAAAAGACTATGTGGAAAAGACATTTCGGAATTTATTGAAAATCAATAACATGATTAATAATGAAACCAGTCGACTTACATAAATCAACAAGGGCGCCTTGACGACAAAGACCAGTACGCTCACAGCCAATGACg < 2:343693/273‑1 (MQ=255) tCAAATTTAATTAAACTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACTACAAAGCCCAGTACGCTCACAGCCAATGACg < 2:430579/275‑1 (MQ=255) aTTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCg > 1:145110/1‑159 (MQ=255) cccTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCAt < 2:145104/159‑1 (MQ=255) ccTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCTATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCGATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGAGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACAAGTACg > 1:311135/1‑274 (MQ=255) agaatTTGATTTCCTAAAGGTCCATTAAGGATATGTGTCATATCGATAACTAAGAAGCCTTCAAACGGCCCTTTGCTTTCATNATTTGTCANTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCTGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGc < 1:335584/275‑1 (MQ=255) tCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATgc > 1:287665/1‑275 (MQ=255) aTAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAAt > 1:129222/1‑193 (MQ=255) cTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACg < 2:129216/193‑1 (MQ=255) gCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAATCCGACGGTGACAAGACACTTCCGCATTTAGGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTAACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTca < 1:291559/174‑1 (MQ=255) cTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACaaa > 1:135539/1‑158 (MQ=255) tCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGAc > 1:97328/1‑126 (MQ=255) aaaCGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGccc < 2:135533/158‑1 (MQ=255) aCGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATaa < 2:97326/126‑1 (MQ=255) gCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCCGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACAGGCTGTCAATAATGATTCCGTATAATCCCTGCGGTAATGCTCCCCCCATTTCCATCctcttcctcttttc > 2:273737/1‑265 (MQ=255) gaattcgttttAATTATTTGTAATTAAATTATGCCTTTAAAAAAGANGAAGGTGAAAAGACACTTCGTCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAAGCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCg < 2:311126/267‑1 (MQ=255) tttGTCATTTGCTTATGCCTGTAAAAAAGCCGAAGGTGAAAAGACACTGCGGCATTGATGTAAAATCAATAACATTATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACATCCAATGACGc < 2:87448/159‑1 (MQ=255) aTTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCCGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATAc < 1:273745/275‑1 (MQ=255) aTGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACCAGTACGGGTGTa > 1:449540/1‑163 (MQ=255) cTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACa < 2:449527/163‑1 (MQ=255) aGCCGAAGGTGAAAGGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACACTCACAGc > 1:75085/1‑123 (MQ=255) acTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCt > 2:272015/1‑115 (MQ=255) acTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACCAGTACgg > 2:409297/1‑127 (MQ=255) cGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTAc < 1:272023/115‑1 (MQ=255) cGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACCAGTACGGgtgt < 1:409310/127‑1 (MQ=255) | GCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGACATGTGTCATATCGATAACTAATAAGCCTTCAAACGGCCCTTTGCTTTCATTATTTGTCATTTGCTTATGCCTTGAAAAAAGCCGAAGGTGAAAAGACACTTCGGCATTTATGTAAAATCAATAACATGATTAATTATGAAACCAGTCGACTGACATAAATCCACAAGGGAGCCGTGACGACAAAGCCCAGTACGCTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATAC > CP009273/2482438‑2482920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |