| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,160,274 | C→A | 12.2% | P268P (CCG→CCT) | ygiS ← | putative inner membrane ABC superfamily transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,160,274 | 0 | C | A | 12.2% | 89.3 / 2.1 | 33 | P268P (CCG→CCT) | ygiS | putative inner membrane ABC superfamily transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (13/16); new base A (2/2); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.15e-01 | |||||||||||
CGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTTTGCGCAATAAGCTGTCGATCAACCGTAAGATATAGCGCCCGACGCACTCGCACATCGTTAAATGGCGGTTTCTCAAGGTTGAAGTTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGTCGGCCA > CP009273/3160037‑3160534 | cgtcgtCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACNTTTTGCGCAATAAGCTGTCGATCAACCGTAAGATATAGCGCCCGACGCACTCGCACATCGTTAAATGGCGGTTTCTCAAGGTTGAAGTTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTgaga < 1:381926/253‑1 (MQ=255) ctggCGTTCTCAACCCCAGTACCTTTTGCGCAATAAGCTGTCGATCAACCGTAAGATATAGCGCCCGACGCACTCGCACATCGTTAAATGGCGGTTTCTCAAGGTTGAAGTTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTg < 1:5173/272‑1 (MQ=255) tctcAACCCCAGTACCTTTTGAGCAATAAGCTGTCGATCAACCGTAAGATATAGCGCTCGATGCACTCGCACATCGTTAAATGGCGTTTTCNCAAGGTTGAAGGTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTGTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCTATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATATTCAACCTGTTGCAATa < 1:372448/275‑1 (MQ=255) cgcAATAAGCTGTCGAACAAACGTAAGATATCGCGCCCGACGCACTCGCACATCGTTAAATGGCGNTTTCTCAAGGTTGAAGTTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTg < 1:378171/249‑1 (MQ=255) tAAGCTGTCGATCAACCGTAAGATATAGCGCCCGACGCACTCGCACATCGTTAAATGGCGGTTTCTCAAGGTTGAAGTTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAgc < 1:290410/224‑1 (MQ=255) gTAAGATATAGCGCCCGACGCACTCGCACATCGTTAAATGGCGGTTTCTCAAGGTTGAAGTTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTAt > 2:397359/1‑202 (MQ=255) gATATAGCGCCCGACGCACTCGCACATCGTTAAATGGCGGTTTCTCAAGGTTGAAGTTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAg < 1:397372/202‑1 (MQ=255) taGCGCCCGACGCACTCGCACATCGTTAAATGGCGGTTTCTCAAGGTTGAAGTTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTATCGCCAGATACTCAACCt > 2:430469/1‑215 (MQ=255) taGCGCCCGACGCACTCGCACATCGTTAAAAGGCGGTTTATCAAGGTTGAGGTTGTAATATTCGCTGTTCCGACGCGGAATAATTCGTAGCTCGCCTGGCACTGATCTTTCACTGGCGGGCATTTGCTGCGCCGGAACCCAGGTGAGCTCGACATCTCCCGAGCGATAACGGTTATAGCCGGTGACCGAATTCGCTAGCGCCAGTTACTCAAACTGTTGCAATACTGTATGTGGCGCATAGCGGTACTTTGGACTTTTGCGTGcgatagttttt > 2:472170/1‑264 (MQ=255) gcCCGACGCACTCGCACATCGTTAAATGGCGGTTTCTCAAGGTTGAAGTTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTg < 1:430482/215‑1 (MQ=255) cGTTAAATGGCGGTTTCTCAAGGTTGAGGTTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGCTAATCAACCTGTTGCAATACTGTATGTTGCGAATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTAACCACTGATCAAGc > 1:291052/1‑275 (MQ=255) ttAAATGGCGGTTTCTCAAGGTTGAAGTTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTCACCACTGATAAAGcac > 1:163574/1‑275 (MQ=255) ggggTTTCTCTGGTGTGCAGTTGTAATGTGTTCTGTTCAGACGCGGAATTATTCGTAGCTCGCCAGGCAGTTATTTTTCAATGGCGGGAATTTGCTGCGCCGGATCCCAGGTGAGAGCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTGTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCa < 2:446504/273‑1 (MQ=255) ggTTGAAGTTGTAATTTTCGCTGTTCCGACTCGGAATAATTCGTTGCTTGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCTCCGGCACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTGACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAAcc < 1:53762/275‑1 (MQ=255) ttGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCg < 1:442888/82‑1 (MQ=255) gTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCGAGCGCCAGCTACTCCACCTGTCGCAATACTGTATGTTGCGCACCGAGTGACTTTGGATGTTTTCGTTCAGTAATATTTTCGTGAGCTAACCACTCATCAAGCACGACGGAACCTTTGTACAAGATGTTCTCTg > 2:433758/1‑275 (MQ=255) cGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCAGGAACCCAGGt > 2:466257/1‑82 (MQ=255) tGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGa > 1:253077/1‑140 (MQ=255) gTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCAGGAACCCAGGTgaga < 1:466270/82‑1 (MQ=255) cAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACt < 2:253070/140‑1 (MQ=255) cgGAATAATTCGTAGCTCGCCAGTCAGTAATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGt > 1:340836/1‑142 (MQ=255) gAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGt > 1:271810/1‑155 (MQ=255) ataatTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCa < 2:340824/142‑1 (MQ=255) aatTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTgcg < 2:271802/155‑1 (MQ=255) atTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCAGGAACCCAGGTGAGATCGACCTCTccc > 2:176822/1‑78 (MQ=255) gTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCAGGAACCCAGGTGAGATCGACCTCTCCcgcgc < 1:176828/78‑1 (MQ=255) cGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCcgcgcg > 2:20358/1‑73 (MQ=255) gCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCcgcgcg > 1:266113/1‑72 (MQ=255) aGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAg < 1:20359/73‑1 (MQ=255) ggCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAg < 2:266105/72‑1 (MQ=255) tgttCACTGGCGGGAATTTGCTGCGCCGGAACCCAGGTTAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGaa < 2:163568/273‑1 (MQ=255) ggAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTCCCCACTGCTCAAGCACAAAGGCACCGTGGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGCAGGccc > 2:454613/1‑274 (MQ=255) cgcCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCg > 1:104601/1‑148 (MQ=255) | CGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTTTGCGCAATAAGCTGTCGATCAACCGTAAGATATAGCGCCCGACGCACTCGCACATCGTTAAATGGCGGTTTCTCAAGGTTGAAGTTGTAATATTCGCTGTTCAGACGCGGAATAATTCGTAGCTCGCCAGGCAGTGATTTTTCAATGGCGGGAATTTGCTGCGCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGTCGGCCA > CP009273/3160037‑3160534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |