Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 774,634 G→T 17.1% G38C (GGT→TGT)  pal → peptidoglycan‑associated outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273774,6340GT17.1% 61.4 / 2.2 26G38C (GGT→TGT) palpeptidoglycan‑associated outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (9/11);  new base T (2/2);  total (12/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

GTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGG  >  CP009273/774456‑774887
                                                                                                                                                                                  |                                                                                                                                                                                                                                                             
gTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCg                                                                                                                                                                                                                                                         >  2:466692/1‑185 (MQ=255)
    aaTTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCAc                                                                                                                                                                                                                                                     <  1:466705/185‑1 (MQ=255)
                                ataataATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAATGCATGATGCATGCCGGCACTNGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGaa                                                                                                                                                                    >  1:444675/1‑238 (MQ=255)
                                    taattaatTGAATAGTAAAGGAAGCATTGAAATGGAACTGAACAAAGGGCTGAAAGGGCGTATGATTGCTCTGCCTGTTATGGCAAGTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGaacaac                                                                                                                                                                <  2:444662/238‑1 (MQ=255)
                                                       ggAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCACTGAATCCCTGCTGAGTGCCGGCACTGGTa                                                                                                                                                                                                                                                <  1:342161/139‑1 (MQ=255)
                                                                                 aGTGCTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCg                                                                                                                                                                                                                                                         >  1:176048/1‑104 (MQ=255)
                                                                                   tGCTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGaac                                                                                                                                                                   >  1:216415/1‑188 (MQ=255)
                                                                                     cTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCAc                                                                                                                                                                                                                                                     <  2:176042/104‑1 (MQ=255)
                                                                                       gAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAacaaca                                                                                                                                                               <  2:216408/188‑1 (MQ=255)
                                                                                                       gCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAATAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGTTGCGCACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCTTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCa                                                         <  1:104826/274‑1 (MQ=255)
                                                                                                                       cAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGcggcaacggca                                                                                                                                                                                                                        >  2:202481/1‑99 (MQ=255)
                                                                                                                       cAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGGCTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAAAAGGTCAcgc                                         >  1:492310/1‑274 (MQ=255)
                                                                                                                           tGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGt                                                                                                                                                                                                                                                             >  2:56033/1‑58 (MQ=255)
                                                                                                                           tGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACAt                                                                                                                                                                                                                    <  1:202487/99‑1 (MQ=255)
                                                                                                                             cGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACt                                                                                                                                                                                                                                                    >  1:31257/1‑65 (MQ=255)
                                                                                                                               gCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCg                                                                                                                                                                                                                                                         <  1:56035/58‑1 (MQ=255)
                                                                                                                                 aTGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTa                                                                                                                                                                                                                                                <  2:31256/65‑1 (MQ=255)
                                                                                                                                            ccaacaaCGCGCGAAATGAAGGCAGAGAAGACATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATGTCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGaa                   <  2:24907/274‑1 (MQ=255)
                                                                                                                                                   ggcaaGCAAGGACGGCAGCGAAGGAAGGCTGGGTGCCGGCACTGGTAGGGATTCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATacaac             <  2:492297/270‑1 (MQ=255)
                                                                                                                                                   cGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAGCGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATGTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACCTGCAAACTTCCTGCGGAGCAACCCGTCTTACAAAGACAACGTAGAAGGTAACGCGGACGAACGTGGTACTCCggcaaaaaac             >  1:126828/1‑266 (MQ=255)
                                                                                                                                                              aCGGCAGCGAAGGCATGCTGTGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAACTCACCGTAGAAGTGCACGCGGACGAAAGTGGTACTCCCGAATacaacggttcgcttaa  >  2:376651/1‑263 (MQ=255)
                                                                                                                                                                ggCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGtt                                                                                                                                                          >  2:208617/1‑120 (MQ=255)
                                                                                                                                                                  cAGCGAAGGCATGCTGTGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTNCCGAAGAGCAGGCTCGTCTACAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTc                        <  1:376664/248‑1 (MQ=255)
                                                                                                                                                                    gCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACt                                                                                                                                                      <  1:208624/120‑1 (MQ=255)
                                                                                                                                                                       aaGGCATGCTGTGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCt                                                                                                                >  2:5028/1‑155 (MQ=255)
                                                                                                                                                                           cATGCTGTGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCaaa                                                                                                            <  1:5028/155‑1 (MQ=255)
                                                                                                                                                                                  |                                                                                                                                                                                                                                                             
GTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGG  >  CP009273/774456‑774887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑