| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,098,628 | G→T | 16.0% | H9N (CAT→AAT) | csgD ← | csgBAC operon transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,098,628 | 0 | G | T | 16.0% | 77.4 / 2.9 | 25 | H9N (CAT→AAT) | csgD | csgBAC operon transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (10/11); new base T (2/2); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01 | |||||||||||
CAATATTTTGATATTGTTGTTTTTCCTGCTCAAAGTATCCTGCCAATAATGGATAAGCTTTTTATCCGCTTCCATCATATCCAGAAGAATAATAGAGCCTGAAGAGATATCGTCCAGAGAACGTTGAATATTATGTAATTTTCCTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGTTGCACTGCTGTGTGTAGTAATAAATCAGCCCTAAATGGGTAAAATATAAAACTAATGGATTACATCTGATTTCAATCTAGCCATTACAAATCTTAA > CP009273/1098398‑1098828 | caaTATTTTGATATTGTTGTTTTTCCTGCTCAAAGTATCCTGCCAATAATGGATAAGCTTTTTATCCGCTTCCATCATATCCAGAAGAATAATAGAGCCTGAAGAGATATCGTCCAGAGAACGTTGAATATTATGTAATTTTCCTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCtttttt > 1:41208/1‑274 (MQ=255) ccAATAATGGATAAGCTTTTTATCCGCTTCCATCATATCCAGAAGAATAATAGAGCCTGAAGAGATATCGTCCAGAGAACGTTGAATATTATGTAATTTTCCTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTAt < 1:257155/235‑1 (MQ=255) aatGGATAAGCTTTTTATCCGCTTCCATCATATCCAGAAGAATAATAGAGCCTGAAGAGATATCGTCCAGAGAACGTTGAATATTATGTAATTTTCCTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGAAGAAACACAGCTTTTTTAATTGATGGCAAACCTGACAACTGCCTCTAAAATAANAGCACCAGAAg > 2:49859/1‑275 (MQ=255) tctgctttgattatatGCAAAAGAATTATAGAGACTGAAGAGATATCGTCCAGAGAACGTTGAATATTATGTAATTTTCTTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGGGAGCTGTTGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGCCAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGTTCCa < 1:466853/263‑1 (MQ=255) tcatATCCAGAAGAATAATAGAGCCTGAAGAGATATCGTCCAGAGAACGTTGAATATTATGTAATTTTCCTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGTTGCACTGCtgtgtg < 1:49861/275‑1 (MQ=255) aagaaTAATAGAGCCTGAAGAGATATCGTCCAGAGAACGTTGAATATTATGTAATTTTCCTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATTAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTaaa > 2:395792/1‑222 (MQ=255) ataataGAGCCTGAAGAGATATCGTCCAGAGAACGTTGAATATTATGTAATTTTCCTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATTAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAg < 1:395805/222‑1 (MQ=255) ccTGAAGAGATATCGTCCAGAGAACGTTGAATATTATGTAATTTTCCTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATTAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGTTGCACTGCTGTGTGTAGTAATAAATCAGCCCTAAATg > 1:139059/1‑275 (MQ=255) ctcgaTATCGTCCAGAGAACGTTGAATATTATGTAATTTTCCTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATTAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGTTGCACTGCTGTGTGTAGTAATAAATCAGCCCTAAATGGGTa < 2:139053/271‑1 (MQ=255) agagATATCGTCCAGAGAACGTTGAATATTATGTAATTTTCCTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGAGTGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGTTGCACTGCTGTGTGTAGTAATAAATCAGCCTTAAATGGGTa < 1:481264/274‑1 (MQ=255) tatCGTCCAGAGAACGTTGAATATTATGTAATTTTCCTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGTTGCACTGCTGTGTGTAGTAATAAATCAGCCCTAAATGGGTAAAatat < 2:41207/275‑1 (MQ=255) cGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTaaaa > 2:225802/1‑151 (MQ=255) cGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGTTGCACTGCTGTGTGTAGTAATAAATCAGCCCTAAATGGGTACAATATAAAAATAATGGATTACATCTGATTTAAATCTAGCCATTACAAATCATaa > 1:50517/1‑275 (MQ=255) tGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGa < 1:225809/151‑1 (MQ=255) tttAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACtt > 2:51053/1‑83 (MQ=255) aGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCAtt < 1:51055/83‑1 (MQ=255) cAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACa > 1:378030/1‑156 (MQ=255) agagCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATg < 2:378017/156‑1 (MQ=255) tCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGt > 1:366658/1‑150 (MQ=255) cTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGTTGCa < 2:366645/150‑1 (MQ=255) cAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCACGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGt > 2:321993/1‑143 (MQ=255) gaagaTTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAATCACCAGAAGTACTGACAGATGTTGCa < 1:322003/143‑1 (MQ=255) tGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTaaaa > 2:159615/1‑84 (MQ=255) ccATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGTTGCACTGCTGTGTGTAGTAATAAATCAGCCCTAAATGGGTAAAATATaaaa > 2:186294/1‑160 (MQ=255) cATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGa < 1:159621/84‑1 (MQ=255) gAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGTTGCACTGCTGTGTGTAGTAATAAATCAGCCCTAAATGGGTAAAATATAAAACTaa < 1:186300/160‑1 (MQ=255) | CAATATTTTGATATTGTTGTTTTTCCTGCTCAAAGTATCCTGCCAATAATGGATAAGCTTTTTATCCGCTTCCATCATATCCAGAAGAATAATAGAGCCTGAAGAGATATCGTCCAGAGAACGTTGAATATTATGTAATTTTCCTGTAATTGCCAGCGATTGTTTAAGGTGCTGCAAGAGAGCTGTCGCCTGCAAAGAAGATTTAGTGATCAACAATAATGTATGACCATGAATACTATGGACTTCATTAAACATGATGAAACCCCGCTTTTTTTATTGATCGCACACCTGACAGCTGCCTCTAAAATAGAAGCACCAGAAGTACTGACAGATGTTGCACTGCTGTGTGTAGTAATAAATCAGCCCTAAATGGGTAAAATATAAAACTAATGGATTACATCTGATTTCAATCTAGCCATTACAAATCTTAA > CP009273/1098398‑1098828 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |