Predicted mutation
evidence seq id position mutation freq annotation gene description
JC CP009273 3,320,960 +TTTATC 55.2% coding (64/630 nt) rlmE ← 23S rRNA U2552 2'‑O‑ribose methyltransferase, SAM‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 332096015 (0.580)18 (0.700)
+TTTATC		 17/202 NT 55.2% coding (64/630 nt) rlmE 23S rRNA U2552 2'‑O‑ribose methyltransferase, SAM‑dependent
?CP009273 3320961 = 15 (0.550)coding (63/630 nt) rlmE 23S rRNA U2552 2'‑O‑ribose methyltransferase, SAM‑dependent

ATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/3320764‑3320960
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGCTGGCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGT  >  CP009273/3320961‑3321187
                                                                                                                                                                                                     ||||||                                                                                                                                                                                                                                   
ATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTTAACATATTTA                                                                                                                                                                                                                                       >  1:267250/1‑201
               CAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCATGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCTTGAAGCCAGCGGATGGAGCTGGCAGAACGCTTCTTACCTGTCCTTTAACTTTCNCATGGGTATAAC                                                                                                                                                >  2:490894/1‑273
                                                      ACATATTTTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAANTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGCTGGCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTATACGCGCCTATTTGG                                                                                                       <  1:481545/275‑1
                                                        ATATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTNTAAACCATGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGCTGGCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTG                                                                                                     <  1:490902/275‑1
                                                              TGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTA                                                                                                                                                                                                                                       >  1:32396/1‑139
                                                              TGACCAACCACCCGGAGCAGCACCAAGGCCGACAACCGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCT                                                                                                                                                                                                                 >  1:516386/1‑161
                                                                  CAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAG                                                                                                                                                                                                             <  2:516376/161‑1
                                                                  CAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTT                                                                                                                                                                                                                                   <  2:32396/139‑1
                                                                                                            GGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGCTGGCAGAACGCTTCTTACCTGTCATTTAACTTTC                                                                                                                                                             >  2:69212/1‑167
                                                                                                                TAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGCTGGCAGAACGCTTCTTACCTGTCATTTAACTTTCCCAT                                                                                                                                                         <  1:69212/167‑1
                                                                                                                       TTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGCTGGCAGAACGCTTCT                                                                                                                                                                                >  2:258223/1‑137
                                                                                                                           TCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGCTGGCAGAACGCTTCTTACC                                                                                                                                                                            <  1:258227/137‑1
                                                                                                                                           CATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGCTGG                                                                                                                                                                                            >  2:335845/1‑105
                                                                                                                                             TCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCT                                                                                                                                                                                                                            >  2:399350/1‑71
                                                                                                                                               AAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGCTGGCAGA                                                                                                                                                                                        <  1:335850/105‑1
                                                                                                                                                 GTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAG                                                                                                                                                                                                                        <  1:399355/71‑1
                                                                                                                                                      AACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGCNGGCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGAAAAAAATACGATGAAGCTGAGTACTAATCTAAAACAGCACCTGA       <  1:491944/275‑1
                                                                                                                                                           GGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGCTGGCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGACAAAAATACGAAGAATGTGAGTACGAAACAAAAANAACACCgtaacggc  >  2:491936/1‑267
                                                                                                                                                               CGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTT                                                                                                                                                                                                                                        <  1:302490/41‑1
                                                                                                                                                                     CGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTG                                                                                                                                                                                                   >  2:31986/1‑72
                                                                                                                                                                         ACCCCTTTTTCTGTGCCTGTTGAACATATTTATCTTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGC                                                                                                                                                                                               <  1:31986/72‑1
                                                                                                                                                                                                     ||||||                                                                                                                                                                                                                                   
ATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/3320764‑3320960
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGGAGCTGGCAGAACGCTTCTTACCTGTCATTTAACTTTCCCATGGGGATAACTCATCGTAACCAATTGCGTAAATTTTTACGCGCCTATTTGGTGATATATGGGAGATGGCGGTAGAATGACCCGTTTTCAATCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGT  >  CP009273/3320961‑3321187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.